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Archives of Dermatological Research

, Volume 295, Issue 3, pp 117–123 | Cite as

The garlic-derived organosulfur component ajoene decreases basal cell carcinoma tumor size by inducing apoptosis

  • C. M. L. J. Tilli
  • A. J. W. Stavast-Kooy
  • J. D. D. Vuerstaek
  • M. R. T. M. Thissen
  • G. A. M. Krekels
  • F. C. S. Ramaekers
  • H. A. M. Neumann
Original Paper

Abstract

Although the therapeutic role of ajoene, an organosulfur compound of garlic, in cardiovascular diseases and mycology has been established, its usefulness in cancer treatment has only recently been suggested. We applied ajoene topically to the tumors of 21 patients with either nodular or superficial basal cell carcinoma (BCC). A reduction in tumor size was seen in 17 patients. Immunohistochemical assays for Bcl-2 expression in a selection of these tumors before and after treatment showed a significant decrease in this apoptosis-suppressing protein. On average, the percentage of tumor cells expressing the proliferation marker Ki-67 was not decreased, which suggests that the action of ajoene is not explained by a cytostatic effect. To obtain further insight into the mode of action of ajoene, the BCC cell line TE354T and a short-term primary culture of BCC were analyzed for apoptosis induction after treatment with the drug. Apoptosis was detected by morphology of the cells and by flow cytometry. Ajoene induced apoptosis in a dose- and time-dependent manner in these cultures. Taking together the results of the in vivo and in vitro studies, we conclude that ajoene can reduce BCC tumor size, mainly by inducing the mitochondria-dependent route of apoptosis.

Keywords

Ajoene Apoptosis Basal cell carcinoma Bcl-2 Cell cycle 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • C. M. L. J. Tilli
    • 1
    • 2
  • A. J. W. Stavast-Kooy
    • 1
    • 2
  • J. D. D. Vuerstaek
    • 1
    • 2
  • M. R. T. M. Thissen
    • 1
    • 2
  • G. A. M. Krekels
    • 1
    • 2
  • F. C. S. Ramaekers
    • 1
    • 3
  • H. A. M. Neumann
    • 1
    • 4
  1. 1.Research Institute Growth and Development (GROW)University of MaastrichtThe Netherlands
  2. 2.Department of DermatologyUniversity Hospital MaastrichtThe Netherlands
  3. 3.Department of Molecular Cell BiologyUniversity of MaastrichtMaastrichtThe Netherlands
  4. 4.Department of Dermatology and VenereologyErasmus University RotterdamThe Netherlands

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