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Archives of Orthopaedic and Trauma Surgery

, Volume 130, Issue 7, pp 945–951 | Cite as

NgR expression in macrophages promotes nerve regeneration after spinal cord injury in rats

  • Zhuangchen Zhu
  • Bin Ni
  • Guodong Yin
  • Fengjin Zhou
  • Jun Liu
  • Qunfeng Guo
  • Xiang Guo
Basic Science

Abstract

Objective

This study aimed to investigate the expression of Nogo-66 receptor (NgR) in macrophages after SCI and clarify its role in neuron regeneration.

Methods

Macrophages harvested from injured spine cord of rats were stained by double immunofluorescence labeling technique to observe the expression of NgR at histological and cellular levels. Macrophages which expressed NgR were constructed in vitro, and then the effects of NgR on macrophage phagocytosis and neuraxon regeneration in three groups (NgR-macrophages group, mock group and normal macrophages group) were studied using Western blot, micro-MTT colorimetry, and LDH assay separately.

Results

The results showed that CD68-positive macrophages in injured tissue of spine cord expressed NgR after double immunofluorescence staining on day 7 after SCI, and so did macrophages isolated and cultured from the injured spine cord. The results of Western blot showed that phagocytosis of macrophages in NgR-macrophages group was much better than that in mock group and normal macrophage group (p < 0.05). And the results of Micro-MTT colorimetry and LDH assay indicated that the capacity of neuraxon regeneration in NgR-macrophages group was significantly higher than that in the other two groups (p < 0.05).

Conclusions

The results suggested that there was NgR expressing in the infiltrated macrophages following SCI, which increased phagocytosis of the macrophages, and promoted post-SCI CNS regeneration in vitro.

Keywords

Nogo receptor Macrophages CNS Regeneration 

Notes

Acknowledgments

Supported by National Natural Science Foundation of China (No. 30872612).

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Zhuangchen Zhu
    • 1
  • Bin Ni
    • 1
  • Guodong Yin
    • 1
  • Fengjin Zhou
    • 1
  • Jun Liu
    • 1
  • Qunfeng Guo
    • 1
  • Xiang Guo
    • 1
  1. 1.Department of Orthopedics, Changzheng HospitalThe Second Military Medical UniversityShanghaiPeople’s Republic of China

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