Archives of Orthopaedic and Trauma Surgery

, Volume 129, Issue 9, pp 1287–1292

Patients with long bone fracture have altered Caveolin-1 expression in their peripheral blood mononuclear cells

Basic Science

DOI: 10.1007/s00402-008-0776-6

Cite this article as:
Tang, PF., Burke, G.A., Li, G. et al. Arch Orthop Trauma Surg (2009) 129: 1287. doi:10.1007/s00402-008-0776-6

Abstract

Introduction

Fracture triggers a cascade of systemic and local responses including inflammatory mediator signaling, chemotaxis, osteogenic cell recruitment, differentiation and proliferation at the fracture site. Early signaling between immune cells and repair cells in fracture repair is not well understood. Caveolin-1, a 21–24 kDa membrane protein plays key roles in transmembrane signaling. This study was to investigate the expression of caveolin-1 in human peripheral blood mononuclear cells (PBMNCs) following long bone fracture.

Methods

PBMNCs were obtained from healthy volunteers or fracture patients at three time points following fracture by density-gradient-centrifugation procedure. Caveolin-1 gene expression and protein characterization was examined by semi-quantitative RT-PCR, immunocytochemistry and Western blot analysis.

Results

Caveolin-1 mRNA and protein was expressed at low levels in the PBMNCs of non-fracture samples. In contrast, caveolin-1 expression was greatly increased in the PBMNCs of fracture patients 9–12 days and reduced at 16–21 days following long bone fracture.

Conclusion

The identification of caveolin-1 in PBMNCs and osteoblasts makes this cellular domain a new focus for further investigation. We speculate that caveolin-1 expression in PBMNCs and osteoblasts play an important role in signal transduction during the early stages of fracture healing and may be an indicator for normal or abnormal fracture repair.

Keywords

Caveolin-1 Caveolae Mononuclear cells Blood Fracture healing 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Pei-Fu Tang
    • 1
  • George A. Burke
    • 2
  • Gang Li
    • 2
    • 3
  • Yan Wang
    • 1
  1. 1.Department of Orthopaedic SurgeryGeneral Hospital of Chinese People’s Liberation ArmyBeijingPeople’s Republic of China
  2. 2.Department of Orthopaedic Surgery, School of Medicine, Dentistry and Biomedical SciencesQueen’s University BelfastBelfastUK
  3. 3.CUHK-WHO Collaborating Centre for Sports Medicine and Health Promotion, Department of Orthopaedics and TraumatologyThe Chinese University Hong Kong, Clinical Sciences Building, Prince of Wales HospitalHong KongPeople’s Republic of China

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