Acta Neuropathologica

, Volume 97, Issue 1, pp 1–4 | Cite as

Human astrocytic brain tumors express APO2L/TRAIL

  • Johannes Rieger
  • Hiroko Ohgaki
  • Paul Kleihues
  • M. Weller
Express communication

Abstract

APO2 ligand (APO2L) is a CD95 ligand (CD95L)-related cytokine of the tumor necrosis factor family that interacts with agonistic (DR4, DR5) and antagonistic (DcR1, DcR2) receptors. Cultured malignant glioma cells preferentially express agonistic receptors and are susceptible to APO2L-induced apoptosis. Here, we report that 8 of 8 human glioma cell lines expressed APO2L mRNA and protein in vitro. Immunohistochemistry using a monoclonal antibody to APO2L revealed that all 23 primary astrocytic brain tumors analyzed, including low-grade astrocytomas and glioblastomas, express APO2L in vivo. With the exception of reactive astrocytes, non-neoplastic glia and neurons in the cerebrum lacked immunoreactivity of APO2L. Thus, in addition to the CD95/CD95L system, a second death ligand/death receptor pair may regulate susceptibility to apoptosis in human glial neoplasms.

Key words APO2 ligand CD95 ligand Glioma 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Johannes Rieger
    • 1
  • Hiroko Ohgaki
    • 2
  • Paul Kleihues
    • 2
  • M. Weller
    • 1
  1. 1.Laboratory of Molecular Neuro-Oncology, Department of Neurology, University of Tübingen, D-72 076 Tübingen, Germany e-mail: michael.weller@uni-tuebingen.de, Tel.: +49-7071-298 2141, Fax: +49-7071-29 6507DE
  2. 2.International Agency for Research on Cancer (IARC), Lyon, FranceFR
  3. 3.Institute of Neuropathology, University Hospital, CH-8091 Zurich, SwitzerlandCH

Personalised recommendations