Acta Neuropathologica

, Volume 94, Issue 4, pp 303–309

Alterations of cell cycle regulatory genes in primary (de novo) and secondary glioblastomas

  • Wojciech Biernat
  • Yasuo Tohma
  • Yasuhiro Yonekawa
  • Paul Kleihues
  • H. Ohgaki
Express communication

DOI: 10.1007/s004010050711

Cite this article as:
Biernat, W., Tohma, Y., Yonekawa, Y. et al. Acta Neuropathol (1997) 94: 303. doi:10.1007/s004010050711

Abstract

Primary glioblastomas develop rapidly de novo through a genetic pathway characterized by amplification/overexpression of EGFR and of MDM2 genes. Secondary glioblastomas develop more slowly through progression from low grade or anaplastic astrocytoma and show a high incidence of a p53 mutation. In the present study, primary and secondary glioblastomas were analyzed for p16 deletions and CDK4 amplification by differential PCR and for loss of expression of the retinoblastoma (RB) gene by immunohistochemistry. Except for one case, alterations in the structure or expression of p16, CDK4 and RB were mutually exclusive. The overall incidence of aberrant expression of these genes coding for components of the cell-cycling-regulatory system was similar in primary (14/28; 50%) and secondary glioblastomas (9/23; 39%). However, p16 deletions were significantly more frequent in the former (10/28; 36%) than in the latter (1/23, 4%; P = 0.0075), suggesting that this alteration constitutes an additional genetic hallmark of the primary (de novo) glioblastoma.

Key wordsp16 CDK4 RB Glioblastoma Progression 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Wojciech Biernat
    • 1
  • Yasuo Tohma
    • 1
  • Yasuhiro Yonekawa
    • 2
  • Paul Kleihues
    • 1
  • H. Ohgaki
    • 1
  1. 1.Unit of Molecular Pathology, International Agency for Research on Cancer, 150 cours Albert Thomas, F-69372 Lyon Cedex 08, France Tel.: 33-472-73-85-34; Fax: 33-472-73-85-64FR
  2. 2.Department of Neurosurgery, University Hospital, Ramistrasse 100, CH-8091 Zurich, SwitzerlandCH

Personalised recommendations