Advertisement

Acta Neuropathologica

, Volume 100, Issue 2, pp 115–121 | Cite as

NACP/α-synuclein and tau constitute two distinctive subsets of filaments in the same neuronal inclusions in brains from a family of parkinsonism and dementia with Lewy bodies: double-immunolabeling fluorescence and electron microscopic studies

  • K. Arima
  • T. Mizutani
  • M. A. Alim
  • H. Tonozuka-Uehara
  • Y. Izumiyama
  • S. Hirai
  • K. Uéda
Regular Paper

Abstract

The co-localization of NACP/α-synuclein and tau epitopes was examined in the brain stem and hippocampal formation in two patients from a family of autosomal dominant parkinsonism and dementia with Lewy bodies (LBs) without two reported missense mutations in the NACP gene. Double-labeling immunofluorescence study revealed that some brain stem LBs, cortical LBs, pale bodies, Lewy-related neurites, and neurofibrillary tangles expressed both NACP epitopes and the PHF tau AT8 epitope. Double-immunolabeling electron microscopy demonstrated that the NACP antibody selectively labeled 9- to 13-nm-thick straight filaments (LB filaments), whereas AT8 recognized twisted tubules with 80- to 100-nm-interval constrictions in the same neuronal inclusions. We show that NACP and tau aggregate into different filamentous components even if both proteins are incorporated into the same inclusions.

Key wordsα-Synuclein Filament pathology Lewy body NACP Tau 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K. Arima
    • 1
  • T. Mizutani
    • 2
  • M. A. Alim
    • 3
  • H. Tonozuka-Uehara
    • 3
  • Y. Izumiyama
    • 1
  • S. Hirai
    • 4
  • K. Uéda
    • 3
  1. 1.Department of Ultrastructure and Histochemistry, Tokyo Institute of Psychiatry, 2-1-8 Kamikitazawa, Setagaya-ku, Tokyo 156-8585, Japan e-mail: arima@prit.go.jp, Tel.: +81-3-33045701, Fax: +81-3-33298035JP
  2. 2.Department of Neurology, Nihon University School of Medicine, Tokyo 173-8610, JapanJP
  3. 3.Department of Neurochemistry, Tokyo Institute of Psychiatry, Tokyo 156-8585, JapanJP
  4. 4.Departments of Laboratory Medicine and Psychiatry, National Center Hospital for Mental, Nervous, and Muscular Disorders, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187-8551, JapanJP

Personalised recommendations