NG2 plays a role in neuroinflammation but is not expressed by immune cells
Nerve/glial antigen 2 (NG2) is a large transmembrane chondroitin sulphate proteoglycan expressed mainly by oligodendrocyte precursor cells in the developing and adult central nervous system (CNS) and pericytes in various organs throughout the body . Numerous studies reported increased levels of this molecule after CNS injury, but its relevance remained questionable [5, 6, 9, 11].
In recent years, contradicting studies have been published concerning the role of NG2 molecule in experimental autoimmune encephalomyelitis (EAE). Using an immunization protocol consisting of two 100 μg doses of MOG35–55, emulsified in CFA, on days 0 and 7 and two injections of 300 ng of pertussis toxin on days 0 and 2 post-immunization, Moransard et al. demonstrated that NG2 is dispensable for EAE development . In contrast, Ferrara et al. reported recently that mice of the same NG2-deficient line displayed milder EAE clinical score . Of note, the latter group used a different immunization protocol...
We thank Michaela Blanfeld, Elena Zurkowski and Alexei Nikolaev for excellent technical support. This work was financed by the Deutsche Forschunsgemeinschaft (DFG) Grant CRC TRR128 to AW, FCK and JT and by European Union, FP7-PEOPLE-2012-ITN NeuroKine to AW.
Compliance with ethical standards
All experiments were performed in accordance with relevant guidelines and regulations of the central animal facility of the Johannes Gutenberg University Mainz.
Conflict of interest
The authors declare that they have no conflict of interest.
- 4.Ferrara G, Errede M, Girolamo F, Morando S, Ivaldi F, Panini N et al (2016) NG2, a common denominator for neuroinflammation, blood-brain barrier alteration, and oligodendrocyte precursor response in EAE, plays a role in dendritic cell activation. Acta Neuropathol 132:23–42CrossRefPubMedPubMedCentralGoogle Scholar