The spectrum of neuropathological changes associated with congenital Zika virus infection
- 1.6k Downloads
A major concern associated with ZIKV infection is the increased incidence of microcephaly with frequent calcifications in infants born from infected mothers. To date, postmortem analysis of the central nervous system (CNS) in congenital infection is limited to individual reports or small series. We report a comprehensive neuropathological study in ten newborn babies infected with ZIKV during pregnancy, including the spinal cords and dorsal root ganglia (DRG), and also muscle, pituitaries, eye, systemic organs, and placentas. Using in situ hybridization (ISH) and electron microscopy, we investigated the role of direct viral infection in the pathogenesis of the lesions. Nine women had Zika symptoms between the 4th and 18th and one in the 28th gestational week. Two babies were born at 32, one at 34 and 36 weeks each and six at term. The cephalic perimeter was reduced in four, and normal or enlarged in six patients, although the brain weights were lower than expected. All had arthrogryposis, except the patient infected at 28 weeks gestation. We defined three patterns of CNS lesions, with different patterns of destructive, calcification, hypoplasia, and migration disturbances. Ventriculomegaly was severe in the first pattern due to midbrain damage with aqueduct stenosis/distortion. The second pattern had small brains and mild/moderate (ex-vacuo) ventriculomegaly. The third pattern, a well-formed brain with mild calcification, coincided with late infection. The absence of descending fibres resulted in hypoplastic basis pontis, pyramids, and cortico-spinal tracts. Spinal motor cell loss explained the intrauterine akinesia, arthrogryposis, and neurogenic muscle atrophy. DRG, dorsal nerve roots, and columns were normal. Lympho-histiocytic inflammation was mild. ISH showed meningeal, germinal matrix, and neocortical infection, consistent with neural progenitors death leading to proliferation and migration disorders. A secondary ischemic process may explain the destructive lesions. In conclusion, we characterized the destructive and malformative consequences of ZIKV in the nervous system, as reflected in the topography and severity of lesions, anatomic localization of the virus, and timing of infection during gestation. Our findings indicate a developmental vulnerability of the immature CNS, and shed light on possible mechanisms of brain injury of this newly recognized public health threat.
KeywordsZika virus Congenital ZIKV infection Microcephaly Calcification In situ hybridization Neuropathology
We thank Débora Silva, Diego Santos, and Luciana Bitana for technical assistance in histological and immuno-histochemical preparations, and Centro Nacional de Biologia Estrutural e Bioimagem (CENABIO) for technical assistance in transmission electron microscopy. These studies received partial support from the National Institute of Health (NIH) IH NIAID grant U01 AI 111598 (C.A.W.) and from Conselho Nacional de Desenvolvimento Científico e Tecnológico [CNPq] and Fundação de Amparo a Pesquisa do Rio de Janeiro [FAPERJ] (A.T).
LC, CAW, ASOM, RSA, and AT designed the study; ASOM, MEM, AGMB, MMRA, FM, TAF, JRLM, and JIBFL managed the patients; FT-M and PSO-S conducted the imaging analysis; GSA, MBA, RMB, and RD did the laboratory studies; AGMB, EA-P, VSL, HNM, CVAM, DCAD, ACGC, and ONU performed the autopsies; LC, AHSC, and EA-P conducted the neuropathological and histopathological investigations. CAW was responsible for the ISH studies; RFMC and SR conducted the ultrastructural studies; LC, CAW, ASOM, FT-M, PSO-S, EA-P, SR, MBA, RMB, RD, RSA, and AT analyzed the results. LC, CAW, FT-M, RSA, and AT wrote and edited the initial drafts. All authors reviewed the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Informed consent was obtained from all parents of participants included in the study.
- 4.Besnard M, Eyrolle-Guignot D, Guillemette-Artur P, Lastère S, Bost-Bezeaud F, Marcelis L et al (2016) Congenital cerebral malformations and dysfunction in fetuses and newborns following the 2013–2014 Zika virus epidemic in French Polynesia. Euro Surveill. doi: 10.2807/1560-7917.ES.2016.21.13.30181 PubMedGoogle Scholar
- 11.de Fatima Vasco Aragao M, van der Linden V, Brainer-Lima AM, Coeli RR, Rocha MA, Sobral da Silva P et al (2016) Clinical features and neuroimaging (CT and MRI) findings in presumed Zika virus related congenital infection and microcephaly: retrospective case series study. BMJ 353:i1901. doi: 10.1136/bmj.i1901 CrossRefPubMedPubMedCentralGoogle Scholar
- 16.Ellison D, Love S, Chimelli LMC, Harding B, Lowe JS, Vinters HV et al (2012) Neuropathology: a reference text of CNS pathology. Elsevier, AmsterdamGoogle Scholar
- 25.Larroche J-C (1977) Developmental pathology of the neonate. Excerpta Medica, Amsterdam, pp 1–21Google Scholar
- 27.Love S, Wiley CA, Lucas S (2015) Viral infections. In: Love S, Perry A, Ironside J, Budka H (eds) Greenfield’s Neuropathology, 9th edn. CRC Press, London, pp 1087–1191Google Scholar
- 41.Soares de Oliveira-Szejnfeld P, Levine D, de O Melo AS, Amorim MMR, Batista AGM, Chimelli L et al (2016) Congenital brain abnormalities and zika virus: what the radiologist can expect to see prenatally and postnatally. Radiology 281:203–218. doi: 10.1148/radiol.2016161584 CrossRefPubMedGoogle Scholar
- 46.Tang H, Hammack C, Ogden SC, Wen Z, Qian X, Li Y et al (2016) Zika virus infects human cortical neural progenitors and attenuates their growth brief report Zika virus infects human cortical neural progenitors and attenuates their growth. Cell Stem Cell 18:587–590. doi: 10.1016/j.stem.2016.02.016 CrossRefPubMedPubMedCentralGoogle Scholar