Acta Neuropathologica

, Volume 127, Issue 6, pp 897–909

Human pontine glioma cells can induce murine tumors

  • Viola Caretti
  • A. Charlotte P. Sewing
  • Tonny Lagerweij
  • Pepijn Schellen
  • Marianna Bugiani
  • Marc H. A. Jansen
  • Dannis G. van Vuurden
  • Anna C. Navis
  • Ilona Horsman
  • W. Peter Vandertop
  • David P. Noske
  • Pieter Wesseling
  • Gertjan J. L. Kaspers
  • Javad Nazarian
  • Hannes Vogel
  • Esther Hulleman
  • Michelle Monje
  • Thomas Wurdinger
Original Paper

DOI: 10.1007/s00401-014-1272-4

Cite this article as:
Caretti, V., Sewing, A.C.P., Lagerweij, T. et al. Acta Neuropathol (2014) 127: 897. doi:10.1007/s00401-014-1272-4

Abstract

Diffuse intrinsic pontine glioma (DIPG), with a median survival of only 9 months, is the leading cause of pediatric brain cancer mortality. Dearth of tumor tissue for research has limited progress in this disease until recently. New experimental models for DIPG research are now emerging. To develop preclinical models of DIPG, two different methods were adopted: cells obtained at autopsy (1) were directly xenografted orthotopically into the pons of immunodeficient mice without an intervening cell culture step or (2) were first cultured in vitro and, upon successful expansion, injected in vivo. Both strategies resulted in pontine tumors histopathologically similar to the original human DIPG tumors. However, following the direct transplantation method all tumors proved to be composed of murine and not of human cells. This is in contrast to the indirect method that included initial in vitro culture and resulted in xenografts comprising human cells. Of note, direct injection of cells obtained postmortem from the pons and frontal lobe of human brains not affected by cancer did not give rise to neoplasms. The murine pontine tumors exhibited an immunophenotype similar to human DIPG, but were also positive for microglia/macrophage markers, such as CD45, CD68 and CD11b. Serial orthotopic injection of these murine cells results in lethal tumors in recipient mice. Direct injection of human DIPG cells in vivo can give rise to malignant murine tumors. This represents an important caveat for xenotransplantation models of DIPG. In contrast, an initial in vitro culture step can allow establishment of human orthotopic xenografts. The mechanism underlying this phenomenon observed with direct xenotransplantation remains an open question.

Keywords

Neoplasms Pontine neoplasms Animal disease model Microglia 

Supplementary material

401_2014_1272_MOESM1_ESM.pptx (6.9 mb)
Supplementary material 1 Supplementary Fig. 1 Histopathological characterization of human and murine VU-DIPG-3 and -5. H&E images of a, b h-VU-DIPG-3, c, d h-VU-DIPG-5, eh m-VU-DIPG-3 (transplant generations 3–10, except h = transplant generation 1) and il m-VU-DIPG-5 (transplant generations 3–10, except k = transplant generation 1). High magnification image of small cell phenotype in (a,d) h-VU-DIPG-3 and -5 and in (e, j) the correspondent m-VU-DIPG-3 and -5. b, f Numerous tumor cells surrounding the tunica adventitia in h-VU-DIPG-3 and m-VU-DIPG-3. The arrow in figure b indicates a fibrin aggregate. c, f and i Leptomeningeal spread in h-VU-DIPG-5 and in m-VU-DIPG-3 and -5. The arrow in figure c points at a leptomeningeal artery presenting thick walls. Note the tumor front invading the surrounding brain parenchyma in figure i. d, g, l Tumor cells clustered around vessels in h-VU-DIPG-3 and m-VU-DIPG-3 and -5. Arrows indicate perivascular growth. g Murine intraparencymal tumors presented areas of more compact growth (asterisk) along with diffuse growth after the first two transplant generation. h Perineuronal satellistosis, the arrow points at the neuronal cell body. k m-VU-DIPG-5 invading the skull bone. Scale barsa, c, d, e, g, h, I and j 10 μm; b 100 μm; f, k and l 20 μm. Supplementary Fig. 2 Metaphase spreads in murine pontine tumors and h-SU-DIPG-VI. a Murine VU-DIPG-3 abnormal metaphase with 41 chromosomes including an apparent dicentric chromosome (arrow). b Murine VU-DIPG-5 polyploid metaphase with 80 chromosomes including two centric fusion chromosomes (arrows). c Murine CNMC-D1 diploid metaphase and d grossly abnormal metaphase. e Human SU-DIPG-VI (in vitro passage 10) tetraploid metaphase spread with arrows indicating some of the abnormal chromosomes present. Murine VU-DIPG-3 and -5 cells were obtained from subcutaneous tumors at transplant generation 3–10; m-CNMC-D1 cells were obtained form pontine xenograft (transplant generation 1) and cultured in vitro for eight passages. Supplementary Fig. 3 Murine array-comparative genomic hybridization raw data. Fluorescent image of m-VU-DIPG-3 hybridized with m-DNA CTRL on (a) a human platform and on (b) a murine platform. Supplementary Table 1 Clinical characteristics of subjects whose DIPG tumors were evaluated for microglia/macrophage markers. WHO refers to World Health Organization and GBM refers to glioblastoma multiforme (grade IV). The clinical characteristics for SU-DIPG-VI are reported in Table 1. Supplementary Table 2 Primer sequences. The following primer was used for sequencing the murine H3F3A region of interest: AGATACATGTGTTCACAAAC. Supplementary Table 3 Human SU-DIPG-VI Control Cortex DNA Short Tandem Repeat (STR) fingerprint (PPTX 7102 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Viola Caretti
    • 1
    • 5
    • 6
    • 7
    • 11
  • A. Charlotte P. Sewing
    • 5
    • 6
    • 7
  • Tonny Lagerweij
    • 5
    • 6
    • 7
  • Pepijn Schellen
    • 5
    • 6
    • 7
  • Marianna Bugiani
    • 8
  • Marc H. A. Jansen
    • 5
    • 7
  • Dannis G. van Vuurden
    • 5
    • 7
  • Anna C. Navis
    • 10
  • Ilona Horsman
    • 9
  • W. Peter Vandertop
    • 6
  • David P. Noske
    • 6
    • 7
  • Pieter Wesseling
    • 7
    • 8
    • 10
  • Gertjan J. L. Kaspers
    • 5
  • Javad Nazarian
    • 3
  • Hannes Vogel
    • 2
  • Esther Hulleman
    • 5
    • 6
    • 7
    • 12
  • Michelle Monje
    • 1
    • 11
  • Thomas Wurdinger
    • 4
    • 6
    • 7
    • 12
  1. 1.Departments of Neurology, Neurosurgery and PediatricsStanford University School of MedicineStanfordUSA
  2. 2.Department of PathologyStanford University School of MedicineStanfordUSA
  3. 3.Research Center for Genetic MedicineChildren’s National Medical CenterWashingtonUSA
  4. 4.Department of NeurologyMassachusetts General Hospital, Harvard Medical SchoolBostonUSA
  5. 5.Department of Pediatric OncologyVU University Medical CenterAmsterdamThe Netherlands
  6. 6.Department of NeurosurgeryVU University Medical CenterAmsterdamThe Netherlands
  7. 7.Neuro-oncology Research GroupVU University Medical CenterAmsterdamThe Netherlands
  8. 8.Department of PathologyVU University Medical CenterAmsterdamThe Netherlands
  9. 9.Department of Clinical GeneticsVU University Medical CenterAmsterdamThe Netherlands
  10. 10.Department of PathologyRadboud University Medical CentreNijmegenThe Netherlands
  11. 11.Stanford University School of MedicineStanfordUSA
  12. 12.VU University Medical CenterAmsterdamThe Netherlands

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