Acta Neuropathologica

, Volume 121, Issue 2, pp 241–252 | Cite as

Mutation-specific IDH1 antibody differentiates oligodendrogliomas and oligoastrocytomas from other brain tumors with oligodendroglioma-like morphology

  • David Capper
  • David Reuss
  • Jens Schittenhelm
  • Christian Hartmann
  • Juliane Bremer
  • Felix Sahm
  • Patrick N. Harter
  • Astrid Jeibmann
  • Andreas von Deimling
Original Paper


Isocitrate dehydrogenase 1 (IDH1) mutations are frequent in astrocytomas, oligoastrocytomas and oligodendrogliomas. We previously reported the generation of a mutation-specific antibody that specifically detects R132H mutated IDH1 protein (clone H09). Here, we investigate the feasibility of H09 immunohistochemistry to differentiate between oligodendrogliomas/oligoastrocytomas and other tumors with similar morphology. A total of 274 brain tumors presenting with focal or extensive clear cell morphology were investigated. High numbers of H09-positive cases were observed in adult grade II oligodendrogliomas (67 of 74, 91%), grade III oligodendrogliomas (65 of 69, 94%), grade II oligoastrocytomas (11 of 14, 79%) and grade III oligoastrocytomas (10 of 11, 91%). All cases of pediatric oligodendrogliomas (n = 7), neurocytomas (n = 41, 35 central, 4 extraventricular, 2 cerebellar liponeurocytomas), dysembryoplastic neuroepithelial tumors (n = 21), clear cell ependymomas (n = 8), clear cell meningiomas (n = 9) as well as 12 primary glioblastomas with oligodendroglial differentiation and 5 pilocytic astrocytomas with oligodendroglial-like differentiation were negative for H09 immunohistochemistry. Three oligodendrogliomas with neurocytic differentiation had evidence of IDH1/IDH2 mutations either by H09 immunohistochemistry or direct sequencing. We conclude that in tumors with an oligodendroglioma-like morphology, binding of H09 is highly specific for oligodendrogliomas or oligoastrocytomas and substantially helps in the discrimination from other clear cell tumors. Negative H09 immunohistochemistry of an adult oligodendroglioma or oligoastrocytoma should prompt the consideration of other clear cell neoplasms. Further, our observations firmly assign oligodendrogliomas with neurocytic differentiation to the group of oligodendrogliomas and demonstrate that H09 is especially helpful for the difficult discrimination of such lesions from extraventricular neurocytomas.


Isocitrate dehydrogenase IDH1 R132H Antibody Oligodendroglioma Clear cell 



We would like to thank Jochen Meyer and Franziska Moessler for excellent technical assistance. This work was supported by the Bundesministerium für Bildung und Forschung (BMBF–01ES0730 and 01GS0883).

Conflict of interest

Under a licensing agreement between DIANOVA GmbH, Hamburg, Germany, and the German Cancer Research Center, Dr. Capper, Dr. Hartmann and Dr. von Deimling are entitled to a share of royalties received by the German Cancer Research Center on the sales of H09 antibody. The terms of this arrangement are being managed by the German Cancer Research Center in accordance with its conflict of interest policies.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • David Capper
    • 1
    • 2
  • David Reuss
    • 1
    • 2
  • Jens Schittenhelm
    • 3
  • Christian Hartmann
    • 1
    • 2
  • Juliane Bremer
    • 4
  • Felix Sahm
    • 1
  • Patrick N. Harter
    • 5
  • Astrid Jeibmann
    • 6
  • Andreas von Deimling
    • 1
    • 2
  1. 1.Department of Neuropathology, Institute of PathologyRuprecht-Karls-Universität HeidelbergHeidelbergGermany
  2. 2.Clinical Cooperation Unit NeuropathologyGerman Cancer Research CenterHeidelbergGermany
  3. 3.Institute of Brain ResearchUniversity Hospital TuebingenTübingenGermany
  4. 4.Institute of NeuropathologyUniversity Hospital of ZürichZürichSwitzerland
  5. 5.Institute of Neurology (Edinger Institute)Goethe University School of MedicineFrankfurt am MainGermany
  6. 6.Institute of NeuropathologyUniversity Hospital MünsterMünsterGermany

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