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Acta Neuropathologica

, Volume 113, Issue 4, pp 451–459 | Cite as

Increased expression of neuregulin-1 in differentiating muscle satellite cells and in motoneurons during muscle regeneration

  • Miyuki Hirata
  • Kunihiro Sakuma
  • Seiichiro Okajima
  • Hiroyoshi Fujiwara
  • Shuichiro Inashima
  • Masahiro Yasuhara
  • Toshikazu Kubo
Original Paper

Abstract

Neuregulins belong to a family of multipotent growth-promoting proteins, and have been shown to have a crucial role in accumulating acetylcholine receptor at neuromuscular junctions. A functional role of neuregulins in muscle regeneration has not yet been identified. Using reverse transcription (RT)-PCR, Western blot and immunofluorescence analysis following bupivacaine injection into rat muscle, we investigated the expression pattern of neuregulin-1 (NRG-1) in normal and regenerating tibialis anterior (TA) muscle. In addition, we examined changes in NRG-1 expression in the spinal cord following muscle damage. Western blotting showed that muscle NRG-1 protein decreased soon after the damage, and increased gradually after the 4th day following the damage. The amount of NRG-1 mRNA in the muscle increased from the 2nd to 6th post-surgical day. The amount of NRG-1 protein, but not mRNA, increased gradually in the spinal cord after muscle damage. Immunofluorescence revealed NRG-1 protein in some quiescent satellite cells identified by c-Met. After 6 and 10 days, clear co-localization between NRG-1 and myogenin was noted in differentiating satellite cells. Thus, NRG-1 may play an important role in the differentiation of satellite cells in muscle regeneration, while increased NRG-1 expression in motoneurons may enhance the remodeling of partially damaged axons.

Keywords

Neuregulin-1 Muscle regeneration Differentiation Spinal cord Satellite cell 

Notes

Acknowledgments

This work was supported by a research Grant-in-Aid for Young Scientists B (No. 17700500) and Scientific Research C (No. 16591505) from the Ministry of Education, Science, Sports and Culture of Japan, and by the grant for young researcher’s project of Research Center for Future Technology, Toyohashi University of Technology.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Miyuki Hirata
    • 1
  • Kunihiro Sakuma
    • 3
  • Seiichiro Okajima
    • 1
  • Hiroyoshi Fujiwara
    • 1
  • Shuichiro Inashima
    • 2
  • Masahiro Yasuhara
    • 2
  • Toshikazu Kubo
    • 1
  1. 1.Department of OrthopedicsKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Department of Legal MedicineKyoto Prefectural University of MedicineKyotoJapan
  3. 3.Health Science CenterToyohashi University of TechnologyToyohashiJapan

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