Rheologica Acta

, Volume 48, Issue 1, pp 109–115 | Cite as

Holographic microrheology of polysaccharides from Streptococcus mutans biofilms

  • Fook Chiong Cheong
  • Simone Duarte
  • Sang-Hyuk Lee
  • David G. Grier
Original Contribution


We use three-dimensional holographic particle tracking to perform microrheological measurements of model gelled media, including the polysaccharide pellicle of dental biofilms created by the common cariogenic oral pathogen Streptococcus mutans. Nanometer-resolution video-rate holographic tracking of embedded colloidal spheres provides accurate measurements of the gels’ complex viscoelastic moduli, including insights into these properties’ heterogeneity. When applied to polysaccharides of S. mutans biofilms, these techniques promise quantitative microscopic assays for candidate therapeutic agents against cariogenic dental biofilms.


Video holographic microscopy Microrheology Biofilms Polysaccharide gel Streptococcus mutans 



This work was supported by the National Science Foundation under Grant Number DMR-0606415.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Fook Chiong Cheong
    • 1
  • Simone Duarte
    • 2
  • Sang-Hyuk Lee
    • 3
  • David G. Grier
    • 1
  1. 1.Department of Physics and Center for Soft Matter ResearchNew York UniversityNew YorkUSA
  2. 2.Department of Basic Science and Craniofacial Biology, College of DentistryNew York UniversityNew YorkUSA
  3. 3.Department of PhysicsUniversity of CaliforniaBerkeleyUSA

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