Holographic microrheology of polysaccharides from Streptococcus mutans biofilms
- First Online:
- 348 Downloads
We use three-dimensional holographic particle tracking to perform microrheological measurements of model gelled media, including the polysaccharide pellicle of dental biofilms created by the common cariogenic oral pathogen Streptococcus mutans. Nanometer-resolution video-rate holographic tracking of embedded colloidal spheres provides accurate measurements of the gels’ complex viscoelastic moduli, including insights into these properties’ heterogeneity. When applied to polysaccharides of S. mutans biofilms, these techniques promise quantitative microscopic assays for candidate therapeutic agents against cariogenic dental biofilms.
KeywordsVideo holographic microscopy Microrheology Biofilms Polysaccharide gel Streptococcus mutans
- Bohren CF, Huffman DR (1983) Absorption and scattering of light by small particles. Wiley, New YorkGoogle Scholar
- Lawrence JR, Korber DR, Hoyle BD, Costerton JW, Caldwell DE (1991) Optical sectioning of microbial biofilms. J Bacteriol 173:6558–6567Google Scholar
- Mason TG, Dhople A, Wirtz D (1997) Concentrated DNA rheology and microrheology. In: MRS proceedings on statistical mechanics in physics and biology, vol 463. Materials Research Society, Pittsburgh, pp 153–158Google Scholar