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Colloid and Polymer Science

, Volume 276, Issue 2, pp 152–159 | Cite as

Effect of denaturants on the stability of light-harvesting complex

  • K. Iida
  • M. Nango
  • H. Yasue
  • K. Okuda
  • M. Okita
  • A. Kashiwada
  • N. Takada
  • M. Maekawa
  • Y. Kurono
ORIGINAL CONTRIBUTION

Abstract

 The effect of denaturants such as urea and normal alcohols on the formation of light-harvesting (LH) polypeptides/bacteriochlorophyll a (BChla) complex (LH1 complex) in n-octyl-β-D-glucopyranoside (OG) micelle was examined to provide an insight into stability of the complex. The stabilities of the LH1 complex in OG micelle and of the complex in the chromatophore of photosynthetic bacteria were compared by addition of denaturants. The extent of stability of these complexes was monitored by the change in absorbance of Qy band of BChla in these complexes, resulting generally in the blue-shifting of the Qy band from near 870 nm to about 777 nm upon addition of these denaturants. Urea and guanidium hydrochloride (Gnd) showed a relatively weak denaturing effect. Normal alcohols showed stronger denaturing effect, depending on the hydrophobicity of the alcohols. These results imply that the stability of LH1 complex in OG micelle can be largely attributed to the hydrophobic interactions in the complex as well as that of the complex in the chromatophore of photosynthetic bacteria.

Key words Denaturants OG micelle bacteriochlorophyll reconstitution light-harvesting complex 

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Copyright information

© Steinkopff Verlag 1998

Authors and Affiliations

  • K. Iida
    • 1
  • M. Nango
    • 2
  • H. Yasue
    • 2
  • K. Okuda
    • 2
  • M. Okita
    • 2
  • A. Kashiwada
    • 2
  • N. Takada
    • 2
  • M. Maekawa
    • 3
  • Y. Kurono
    • 4
  1. 1.Nagoya Municipal Industrial Research Institute Atsuta-ku, Nagoya 456, JapanJP
  2. 2.Department of Applied Chemistry Nagoya Institute of Technology Gokiso-cho, Showa-ku Nagoya 466, Japan E-mail: nango@ach.nitech.ac.jpJP
  3. 3.Division of life Science and Human Technology Nara Women’s University Nara 630, JapanJP
  4. 4.Department of Pharmaceutics Faculty of Pharmaceutical Science Nagoya City University 3-1 Tanabe-dori, Mizuho-ku Nagoya 46, JapanJP

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