Colloid and Polymer Science

, Volume 297, Issue 1, pp 155–163 | Cite as

Effect of amino acid on calcium phosphate phase transformation: attenuated total reflectance-infrared spectroscopy and chemometrics

  • Yuta OtsukaEmail author
  • Akira Ito
  • Masaki Takeuchi
  • Hideji Tanaka
Original Contribution


The effects of amino acids on calcium phosphate phase transformation were investigated by using attenuated total reflection (ATR)-infrared (IR) spectroscopy. A self-setting apatite cement, which consisted of tetracalcium phosphate and dicalcium phosphate, was transformed into hydroxyapatite (HAp) by kneading with a phosphoric acid solution. Phosphate buffer (0.2 M, pH 6.8) or aqueous solutions of 0.1 w/v% amino acids (alanine, asparagine, serine, and o-phospho-l-serine) were used as the kneading solutions. The kneaded samples were analyzed by ATR-IR spectroscopy. The amount of HAp produced was determined by the multivariate curve resolution alternative least-squares method based on the ATR-IR spectra. The phase transformations to HAp were fitted to several solid-state kinetic models. The results indicate that o-phospho-l-serine inhibits the crystal growth of HAp. A simulation model of HAp surface interaction was developed by using the molecular dynamics method. The total energy of the HAp surface with amino acids was sufficient to explain the o-phospho-l-serine inhibition model. The inhibition is attributed to the specific adsorption of o-phospho-l-serine on the surface of HAp seed crystals.


Calcium phosphate Hydroxyapatite Phase transformation Attenuated total reflectance-Fourier transform infrared spectroscopy Multivariate curve resolution alternating least squares 


Funding information

This work was supported by JSPS KAKENHI Grant Number JP18H06115.

Compliance with ethical standards

This work complies with ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yuta Otsuka
    • 1
  • Akira Ito
    • 2
  • Masaki Takeuchi
    • 3
  • Hideji Tanaka
    • 3
  1. 1.Faculty of Pharmaceutical SciencesTokyo University of SciencesNodaJapan
  2. 2.Graduate School of Pharmaceutical SciencesTokushima UniversityTokushimaJapan
  3. 3.Institute of Biomedical SciencesTokushima UniversityTokushimaJapan

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