Competitive adsorption of lysozyme and non-ionic surfactants (Brij-35 and pluronic P123) from a mixed solution at water-air and water-xylene interfaces
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The adsorption of lysozyme in the presence of high molecular weight (pluronic P123) and low molecular weight (Brij-35) non-ionic surfactants was studied at liquid-liquid and liquid-air interfaces. Using tritium-labeled compounds and liquid scintillation spectrometry of tritium, we investigated the competitive adsorption of lysozyme-pluronic P123 and lysozyme-Brij-35 at the aqueous-xylene interface. The substitution of protein by both polymer and low molecular weight surfactant was observed, while the presence of lysozyme does not influence the behavior of non-ionic molecules. We found that the ionic strength of the aqueous phase does not influence the strength of the effect, while it has a significant influence on the rate of diffusion and penetration of the adsorption layer by free protein. The thermodynamics of the competitive adsorption was described by the model suggested by Fainerman and co-authors, and the obtained parameters were used to describe the interfacial tension isotherms of the respective mixtures at the aqueous-air interface. Thus, the adsorption of each component of the mixture (protein and non-ionic surfactant) at the aqueous-air interface was revealed.
KeywordsLysozyme Non-ionic surfactants Pluronic P123 Adsorption Radioactive tracers Liquid-liquid interface
This work was supported by a Grant of the President of the Russian Federation # MK-4881.2016.3.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Nylander T, Arnebrant T, Bos M, Wilde P (2008) Protein/emulsifier interactions. In Food Emulsifiers and Their Applications, 2nd edn. Springer, pp 89–171Google Scholar
- 7.Kotsmar CS, Pradines V, Alahverdjieva VS, Aksenenko EV, Fainerman VB, Kovalchuk VI, Krägel J, Leser ME, Noskov BA, Miller R (2009) Thermodynamics, adsorption kinetics and rheology of mixed protein–surfactant interfacial layers. Adv Colloid Interf Sci 150(1):41–54. https://doi.org/10.1016/j.cis.2009.05.002 CrossRefGoogle Scholar
- 11.Lotfi M, Javadi A, Lylyk SV, Bastani D, Fainerman VB, Miller R (2015) Adsorption of proteins at the solution/air interface influenced by added non-ionic surfactants at very low concentrations for both components. 1. Dodecyl dimethyl phospine oxide. Colloids Surf A Physicochem Eng Asp 475:62–68. https://doi.org/10.1016/j.colsurfa.2014.12.065 CrossRefGoogle Scholar
- 12.Fainerman VB, Lotfi M, Javadi A, Aksenenko EV, Tarasevich YI, Bastani D, Miller R (2014) Adsorption of proteins at the solution/air interface influenced by added nonionic surfactants at very low concentrations for both components. 2. Effect of different surfactants and theoretical model. Langmuir 30(43):12812–12818. https://doi.org/10.1021/la502964y CrossRefGoogle Scholar
- 13.Fainerman VB, Aksenenko EV, Lylyk SV, Lotfi M, Miller R (2015) Adsorption of proteins at the solution/air interface influenced by added nonionic surfactants at very low concentrations for both components. 3. Dilational surface rheology. J Phys Chem B 119(9):3768–3775. https://doi.org/10.1021/acs.jpcb.5b00136 CrossRefGoogle Scholar
- 14.Lu W, Zhang G, Wei F, Li W, Cheng K, Ding F, Zhang J, Zheng W (2017) Shape-controlled synthesis of Pd nanocrystals in an aqueous solution by using amphiphilic triblock copolymers as both the stabilizer and the reductant. Polym Sci 295(4):703–707. https://doi.org/10.1007/s00396-017-4041-9 Google Scholar
- 19.Ghosh S, Kuchlyan J, Banik D, Kundu N, Roy A, Banerjee C, Sarkar N (2014) Organic additive, 5-methyl salicylic acid, induces spontaneous structural transformation of aqueous pluronic triblock copolymer solution: a spectroscopic investigation of interaction of curcumin with pluronic micellar and vesicular aggregates. J Phys Chem B 118(39):11437–11448. https://doi.org/10.1021/jp507378w CrossRefGoogle Scholar
- 20.Alexandridis P, Holzwarth JF, Hatton TA (1994) Micellization of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymers in aqueous solutions: thermodynamics of copolymer association. Macromolecules 27(9):2414–2425. https://doi.org/10.1021/ma00087a009 CrossRefGoogle Scholar
- 21.Ganguly R, Kunwar A, Dutta B, Kumar S, Barick KC, Ballal A, Aswal VK, Hassan PA (2017) Heat-induced solubilization of curcumin in kinetically stable pluronic P123 micelles and vesicles: an exploit of slow dynamics of the micellar restructuring processes in the aqueous pluronic system. Colloids Surf B: Biointerfaces 152:176–182. https://doi.org/10.1016/j.colsurfb.2017.01.023 CrossRefGoogle Scholar
- 27.Alahverdjieva VS, Grigoriev DO, Ferri JK, Fainerman VB, Aksenenko EV, Leser ME, Michel M, Miller R (2008) Adsorption behaviour of hen egg-white lysozyme at the air/water interface. Colloids Surf A Physicochem Eng Asp 323(1-3):167–174. https://doi.org/10.1016/j.colsurfa.2007.12.031 CrossRefGoogle Scholar
- 28.Alahverdjieva VS, Grigoriev DO, Fainerman VB, Aksenenko EV, Miller R, Möhwald H (2008) Competitive adsorption from mixed hen egg-white lysozyme/surfactant solutions at the air-water interface studied by tensiometry, ellipsometry, and surface dilational rheology. J Phys Chem B 112(7):2136–2143. https://doi.org/10.1021/jp074753k CrossRefGoogle Scholar