Colloid and Polymer Science

, Volume 295, Issue 11, pp 2197–2205 | Cite as

Polymeric vehicles for transport and delivery of DNA via cationic micelle template method

  • Emi Haladjova
  • Mariya Kyulavska
  • Jordan Doumanov
  • Tanya Topouzova-Hristova
  • Petar Petrov
Original Contribution
First Online:
Received:
Revised:
Accepted:

Abstract

This work describes the preparation of polymeric non-viral system for transport and delivery of nucleic acids. An amphiphilic poly(2-(dimethylamino)ethyl methacrylate)-block-poly(ε-caprolactone)-block-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA20-b-PCL70-b-PDMAEMA20) triblock copolymer was synthesized and used for formation of cationic micelles and subsequent complexation with DNA. Next, a protective polymer coating on the complex surface and removal of copolymer from the particle interior were conducted. In this way, polymer nanocapsules containing DNA molecules were obtained. The synthesized polymer, cationic micelles, complexes, and polymer capsules were investigated by proton nuclear magnetic resonance, gel permeation chromatography, dynamic and electrophoretic light scattering, and transmission electron microscopy. In vitro cytotoxicity assessment of the different systems revealed very good tolerance to human cells.

Keywords

Micelleplexes Polymer carriers DNA Gene delivery 
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Notes

Funding information

This work was supported by the Bulgarian National Science Fund (Project T02/7-2014).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Emi Haladjova
    • 1
  • Mariya Kyulavska
    • 1
  • Jordan Doumanov
    • 2
  • Tanya Topouzova-Hristova
    • 2
  • Petar Petrov
    • 1
  1. 1.Institute of PolymersBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Faculty of BiologySofia University “St. Kliment Ohridski”SofiaBulgaria

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