Design of nucleic acid-layered double hydroxide nanohybrids
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We report here on the design of inorganic–organic hybrid materials, which consist of layered double hydroxides (LDHs) as inorganic carrier and short single-stranded nucleic acids (ssDNA) as organic molecules. LDHs were prepared by the co-precipitation method followed by hydrothermal treatment. A model 12-nucleotide-long sequence was immobilized either by ion exchange or covalent grafting. Both the LDH composition and the nucleic acid-to-particle ratio were optimized throughout the synthesis to develop highly stable suspensions of the hybrid materials. Structural characterization revealed that the covalent attachment of the ssDNA was successfully achieved via silanization of the LDHs in aqueous suspension. Covalent linkage of the nucleic acids confers to this model nanoparticulate system promising properties and potential for applications as therapeutic agents. Fragments of nucleic acids could be introduced into living cells without release during the delivery process since LDHs slowly dissolve in the slightly acidic intracellular space.
KeywordsLayered double hydroxide Nucleic acid Biohybrid Colloids Nanoparticles Inorganic oxides Colloidal stability
This research was sponsored by the Swiss National Science Foundation (150162), Swiss Secretariat for Education, Research and Innovation (C15.0024), COST Action CM1303 and the University of Geneva. Financial support for M. Á.-S. was also provided by the Swiss Scientific Exchange Program (14033). The authors are also grateful to Professor Michal Borkovec for providing access to the instruments for electrophoretic and DLS measurements.
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Conflict of interest
The authors declare that they have no conflict of interest.
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