Colloid and Polymer Science

, Volume 289, Issue 5–6, pp 659–666 | Cite as

Controlling biotinylation of microgels and modeling streptavidin uptake

  • Yaqin Xu
  • Lizanne Pharand
  • Quan Wen
  • Ferdinand Gonzaga
  • Yingfu Li
  • M. Monsur Ali
  • Carlos D. M. Filipe
  • Robert PeltonEmail author
Original Contribution


Compared are two approaches for the biotinylation of poly(N-isopropylacrylamide-co-vinylacetic acid) microgels, 300-nm diameter, water swollen particles with a corona of carboxyl groups. The biotinylated microgels are a platform for bioactive water-based ink. Streptavidin binding was measured as a function of biotin density, and the results were interpreted with a new model that predicts the minimum local density of biotins required to capture a streptavidin. An amino-polyethylene glycol derivative of biotin gave higher biotin contents than a biotin hydrazide. However, the streptavidin content versus biotin content results for both biotin derivatives fell on the same master curve with maximum biotin coverage of 0.11 mg of bound streptavidin per milligram of biotinylated microgel. Exclusion experiments showed that streptavidin was too big to penetrate the cross-linked microgel structure; thus, the conjugated streptavidin was restricted to the microgel surface. The colloidal stability of the microgels was preserved, and the biotinylated products showed good hydrolytic stability.


Microgel Biotinylation Bioconjugation Modeling 



Prof. Todd Hoare is thanked for useful discussions. The authors acknowledge the SENTINEL Bioactive Paper NSERC Network for financial support. RP and YL hold Canada Research Chairs.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yaqin Xu
    • 1
  • Lizanne Pharand
    • 1
  • Quan Wen
    • 1
  • Ferdinand Gonzaga
    • 2
  • Yingfu Li
    • 2
  • M. Monsur Ali
    • 2
  • Carlos D. M. Filipe
    • 1
  • Robert Pelton
    • 1
    Email author
  1. 1.Department of Chemical EngineeringMcMaster UniversityHamiltonCanada
  2. 2.Department of Biochemistry and Biomedical SciencesMcMaster UniversityHamiltonCanada

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