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Colloid and Polymer Science

, Volume 285, Issue 13, pp 1509–1514 | Cite as

Coating of nanoparticles bearing amino groups on the surface with hydrophilic HPMA-based polymers

  • Richard LagaEmail author
  • Čestmír Koňák
  • Vladimír Šubr
  • Karel Ulbrich
Short Communication

Abstract

Two hydrophilic polymer systems, multivalent N-(2-hydroxypropyl)methacrylamide-based copolymers bearing thiazolidine-2-thione (TT) reactive groups randomly distributed along the polymer chain and monovalent semitelechelic pHPMA with the TT end group, were designed for surface modification of gene delivery vectors, namely, DNA polyelectrolyte complexes (PECs) and adenoviruses. In this study, the amino group-modified polystyrene latex nanoparticles were selected as a suitable model of a nanoparticulate delivery system bearing NH2 groups on the surface. The coating process was monitored by changes in molecular weight and hydrodynamic parameters of the nanoparticles by light scattering methods. It was shown that for study of the coating process the model latex particles are more suitable than the original PEC vectors due to better chemical and physical stability of latexes. The results obtained in the model study (reaction conditions, methods of evaluation) suggest an optimal polymer structure and a method of efficient and complete coating of nanoparticle surface well applicable to the real gene delivery vectors.

Keywords

Polyelectrolyte complex Latex Nanoparticle Coating Light scattering 

Abbreviations

DNA

sodium salt of deoxyribonucleic acid from calf thymus

HEPES

4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid

PEC

polyelectrolyte complex

pHPMA

poly[N-(2-hydroxypropyl)methacrylamide]

PLL

poly(l-lysine hydrobromide)

TT

thiazolidine-2-thione

Mw

weight-average molecular weight

Mwa

apparent weight-average molecular weight

Rh

hydrodynamic radius (nm)

ζ

zeta potential of particles (mV)

c

concentration (g ml−1)

dn/dc

refractive index increment

φ

positive/negative charge ratio

dct

surface density of coating polymer (g nm−2 mol−1)

nct

number of coating molecules fixed on particles surface

Subscripts and superscripts

cp

coating polymer

pc

uncoated polyelectrolyte complexes (PEC)

cc

coated PEC

ct

coating layer of latexes and PECs

lx

uncoated latex particles

cl

coated latex particles

Definitions

\({\text{ $ \Delta $ }}M^{{{\text{ct}}}}_{{\text{w}}} = \)

\( M^{{{\text{cc}}}}_{{\text{w}}} - M^{{{\text{pc}}}}_{{\text{w}}} \)

\({\text{ $ \Delta $ }}R_{{\text{h}}} = \)

\( R^{{{\text{cc}}}}_{{\text{h}}} - R^{{{\text{pc}}}}_{{\text{h}}} \)

Notes

Acknowledgements

Financial support of the Grant Agency of the Academy of Sciences of the Czech Republic (AVOZ400500505) and EU (GIANT, No. 512087) is gratefully acknowledged.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Richard Laga
    • 1
    Email author
  • Čestmír Koňák
    • 1
  • Vladimír Šubr
    • 1
  • Karel Ulbrich
    • 1
  1. 1.Institute of Macromolecular ChemistryAcademy of Sciences of the Czech RepublicPrague 6Czech Republic

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