Poly(ethylene oxide)-coated polyamide nanoparticles degradable by glutathione
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Poly(adipoyl chloride-alt-cystamine) nanoparticles covalently coated with poly(ethylene oxide) (M w = 2,000 or 5,000) were prepared as a model of glutathione-degradable drug, protein, or deoxyribonucleic acid delivery systems. The polyamide forming the micellar core has disulfide bonds in the main chain and thus its degradation gives low molecular weight products. The degradation of nanoparticles by the action of glutathione in the reduced or oxidized forms was monitored by time dependencies of the light scattering intensity at 37 °C in media modeling intracellular environment. The lifetimes of nanoparticles were determined. The reductive degradation of the nanoparticles by the reaction with reduced glutathione is an order of magnitude faster than their spontaneous degradation. The effect of nanoparticle concentration and solution pH was also investigated.
KeywordsDisulfide bond Nanoparticles Glutathione Poly(ethylene oxide) Light scattering
Financial support of the Grant Agency of the Academy of Sciences of the Czech Republic (grants A100500501, A4050403, and A400480616) and of the Research Centers program of the Ministry of Education, Youth and Sports (grant no. IM 463 560 8802) is gratefully acknowledged.
- 2.Bremer HJ, Duran M, Kameling JP (1981) Glutathione. In: Bremer HJ, Duran M, Kamerling JP (eds) Disturbances of amino acid metabolism: clinical chemistry and diagnosis. Urban & Schwarzenberg, Baltimore-Munich, pp 80–82Google Scholar
- 6.Chawla RK, Lewis FW, Kutner MH (1984) Gastroenterology 87:770Google Scholar