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Colloid and Polymer Science

, Volume 284, Issue 6, pp 604–610 | Cite as

Forward and backward extractions of cytochrome c using reverse micellar system of sucrose fatty acid ester

  • Hidetaka NoritomiEmail author
  • Saori Ito
  • Naoki Kojima
  • Satoru Kato
  • Kunio Nagahama
Original Contribution

Abstract

The effects of solvent, pH, and ionic strength on the reverse micellar extraction of cytochrome c have been examined, when sucrose fatty acid esters were employed as surfactants of reverse micelles. The transparent and stable reverse micellar organic phase was formed, when the mixture of isooctane and n-butanol (7:3 v/v) was used as the bulk organic phase. The high forward extraction ratio was obtained under mild alkaline and low ionic strength conditions, while the high backward extraction ratio was obtained for acidic pH values or at high ionic strength. The activity of cytochrome c recovered from the reverse micellar phase was sufficiently retained.

Keywords

Reverse micelle Extraction Protein Nonionic surfactant Sucrose fatty acid ester 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Hidetaka Noritomi
    • 1
    Email author
  • Saori Ito
    • 1
  • Naoki Kojima
    • 1
  • Satoru Kato
    • 1
  • Kunio Nagahama
    • 1
  1. 1.Division of Applied ChemistryTokyo Metropolitan UniversityHachioji, TokyoJapan

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