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Basic Research in Cardiology

, Volume 95, Issue 6, pp 479–484 | Cite as

Evidence for an essential role of cyclooxygenase-2 as a mediator of the late phase of ischemic preconditioning in mice

  • Yiru Guo
  • Weike Bao
  • Wen-Jian Wu
  • Ken Shinmura
  • Xian-Liang Tang
  • Roberto Bolli
ORIGINAL CONTRIBUTION

Abstract

Recent studies have demonstrated that cyclooxygenase-2 (COX-2) is an essential mediator of the cardioprotective effects of the late phase of ischemic preconditioning (PC) in rabbits. The goal of this study was to determine whether COX-2 also plays an essential role in late PC in the mouse. B6129F2/J mice underwent a 30-min coronary occlusion followed by 24 h of reperfusion. Administration of the COX-2 selective inhibitor, NS-398, 30 min prior to the 30-min occlusion (5 mg/kg i.p.) had no appreciable effect on infarct size compared with untreated controls (58.8 ± 2.1%, vs. 58.8 ± 4.3% of the risk region, respectively). When mice were preconditioned with six cycles of 4-min coronary occlusion/4-min reperfusion 24 h prior to the 30-min occlusion, infarct size was markedly reduced (19.3 ± 3.4%), indicating a late PC effect. The protective effect of late PC was completely abrogated by administration of NS-398 30 min before the 30-min coronary occlusion (67.7 ± 3.0%), but not by administration of vehicle alone (23.6 ± 3.7%). These results indicate that COX-2 mediates the late phase of ischemic PC in the mouse and imply that the role of this enzyme in cardioprotection is not species-specific.

Key words Ischemic preconditioning – COX-2 – myocardial infarction – mice 
Abbrevations COX cyclooxygenase, iNOS inducible NOS, LV left ventricle or left ventricular, NOS nitric oxide synthase, PC preconditioning 

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Copyright information

© Steinkopff Verlag 2000

Authors and Affiliations

  • Yiru Guo
    • 1
  • Weike Bao
    • 1
  • Wen-Jian Wu
    • 1
  • Ken Shinmura
    • 1
  • Xian-Liang Tang
    • 1
  • Roberto Bolli
    • 1
  1. 1.University of Louisville, Division of Cardiology. Louisville, Kentucky 40292, USA, E-mail: rbolli@Louisville.eduUS

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