Deletion of the last five C-terminal amino acid residues of connexin43 leads to lethal ventricular arrhythmias in mice without affecting coupling via gap junction channels

  • Indra Lübkemeier
  • Robert Pascal Requardt
  • Xianming Lin
  • Philipp Sasse
  • René Andrié
  • Jan Wilko Schrickel
  • Halina Chkourko
  • Feliksas F. Bukauskas
  • Jung-Sun Kim
  • Marina Frank
  • Daniela Malan
  • Jiong Zhang
  • Angela Wirth
  • Radoslaw Dobrowolski
  • Peter J. Mohler
  • Stefan Offermanns
  • Bernd K. Fleischmann
  • Mario Delmar
  • Klaus Willecke
Original Contribution


The cardiac intercalated disc harbors mechanical and electrical junctions as well as ion channel complexes mediating propagation of electrical impulses. Cardiac connexin43 (Cx43) co-localizes and interacts with several of the proteins located at intercalated discs in the ventricular myocardium. We have generated conditional Cx43D378stop mice lacking the last five C-terminal amino acid residues, representing a binding motif for zonula occludens protein-1 (ZO-1), and investigated the functional consequences of this mutation on cardiac physiology and morphology. Newborn and adult homozygous Cx43D378stop mice displayed markedly impaired and heterogeneous cardiac electrical activation properties and died from severe ventricular arrhythmias. Cx43 and ZO-1 were co-localized at intercalated discs in Cx43D378stop hearts, and the Cx43D378stop gap junction channels showed normal coupling properties. Patch clamp analyses of isolated adult Cx43D378stop cardiomyocytes revealed a significant decrease in sodium and potassium current densities. Furthermore, we also observed a significant loss of Nav1.5 protein from intercalated discs in Cx43D378stop hearts. The phenotypic lethality of the Cx43D378stop mutation was very similar to the one previously reported for adult Cx43 deficient (Cx43KO) mice. Yet, in contrast to Cx43KO mice, the Cx43 gap junction channel was still functional in the Cx43D378stop mutant. We conclude that the lethality of Cx43D378stop mice is independent of the loss of gap junctional intercellular communication, but most likely results from impaired cardiac sodium and potassium currents. The Cx43D378stop mice reveal for the first time that Cx43 dependent arrhythmias can develop by mechanisms other than impairment of gap junction channel function.


Connexin43 Zonula occludens protein-1 Nav1.5 Intercalated disc 

Supplementary material

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Supplementary material 1 (PDF 242 kb)
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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Indra Lübkemeier
    • 1
  • Robert Pascal Requardt
    • 2
  • Xianming Lin
    • 3
  • Philipp Sasse
    • 4
  • René Andrié
    • 5
  • Jan Wilko Schrickel
    • 5
  • Halina Chkourko
    • 3
  • Feliksas F. Bukauskas
    • 6
  • Jung-Sun Kim
    • 7
  • Marina Frank
    • 1
  • Daniela Malan
    • 4
  • Jiong Zhang
    • 8
  • Angela Wirth
    • 9
  • Radoslaw Dobrowolski
    • 10
  • Peter J. Mohler
    • 11
  • Stefan Offermanns
    • 9
  • Bernd K. Fleischmann
    • 4
  • Mario Delmar
    • 3
  • Klaus Willecke
    • 1
  1. 1.Life and Medical Sciences (LIMES) Institute, Molecular GeneticsUniversity of BonnBonnGermany
  2. 2.Center for Sepsis Control and Care (CSCC)Jena University HospitalJenaGermany
  3. 3.Leon H. Charney Division of CardiologyNew York University Medical SchoolNew YorkUSA
  4. 4.Institute of Physiology I, Life and Brain CenterUniversity of BonnBonnGermany
  5. 5.Institute of Medicine-CardiologyUniversity of BonnBonnGermany
  6. 6.Dominick P. Purpura Department of NeuroscienceAlbert Einstein College of MedicineNew YorkUSA
  7. 7.Department of Pathology, Samsung Medical CenterSungkyunkwan University School of MedicineSeoulKorea
  8. 8.Institute of Cellular NeurosciencesUniversity of BonnBonnGermany
  9. 9.Max Planck Institute for Heart and Lung ResearchBad NauheimGermany
  10. 10.Department of Biological SciencesRutgers UniversityNewarkUSA
  11. 11.Departments of Internal Medicine and Physiology, Dorothy M. Davis Heart and Lung Research InstituteOhio State University Medical CenterColumbusUSA

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