Basic Research in Cardiology

, 108:318 | Cite as

RETRACTED ARTICLE: Postconditioning promotes the cardiac repair through balancing collagen degradation and synthesis after myocardial infarction in rats

  • Zhang-Feng Wang
  • Ning-Ping Wang
  • Suzanna Harmouche
  • Tiji Philip
  • Xue-Fen Pang
  • Feng Bai
  • Zhi-Qing Zhao
Original Contribution

Abstract

Postconditioning (Postcon) reduces infarct size. However, its role in modulation of cardiac repair after infarction is uncertain. This study tested the hypothesis that Postcon inhibits adverse cardiac repair by reducing degradation of extracellular matrix (ECM) and synthesis of collagens via modulating matrix metalloproteinase (MMP) activity and transforming growth factor (TGF) β1/Smad signaling pathway. Sprague–Dawley rats were subjected to 45 min ischemia followed by 3 h, 7 or 42 days of reperfusion, respectively. In acute studies, four cycles of 10/10 s Postcon significantly reduced infarct size, which was blocked by administration of a mitochondrial KATP channel blocker, 5-hydroxydecanoate (5-HD) at reperfusion. In chronic studies, Postcon inhibited MMP activity and preserved ECM from degradation as evidenced by reduced extent of collagen-rich scar and increased mass of viable myocardium. Along with a reduction in collagen synthesis and fibrosis, Postcon significantly down-regulated expression of TGFβ1 and phospho-Smad2/3, and up-regulated Smad7 as compared to the control, consistent with a reduction in the population of α-smooth muscle actin expressing myofibroblasts within the infarcted myocardium. At 42 days of reperfusion, echocardiography showed significant improvements in left ventricular end-diastolic volume and ejection fraction. The wall thickness of the infarcted middle anterior septum in the Postcon was also significantly greater than that in the control. The beneficial effects of Postcon on cardiac repair were comparable to preconditioning and still evident after a blockade with 5-HD. These data suggest that Postcon is effective to promote cardiac repair and preserve cardiac function; protection is potentially mediated by inhibiting ECM degradation and collagen synthesis.

Keywords

Cardiac repair Collagen Extracellular matrix Postconditioning TGFβ1/Smad pathway 

Notes

Acknowledgments

This study was supported in part by a seed Grant from the Mercer University School of Medicine and National Natural Science Foundation of China (81170145/H0203).

Conflict of interest

No conflicts of interest were declared.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Zhang-Feng Wang
    • 1
    • 3
  • Ning-Ping Wang
    • 1
  • Suzanna Harmouche
    • 1
  • Tiji Philip
    • 1
  • Xue-Fen Pang
    • 2
  • Feng Bai
    • 2
  • Zhi-Qing Zhao
    • 1
    • 2
    • 4
  1. 1.Cardiovascular Research LaboratoryMercer University School of MedicineSavannahUSA
  2. 2.Department of PhysiologyShanxi Medical UniversityTaiyuanChina
  3. 3.Department of OtolaryngologyFirst Affiliated Hospital of Sun Yat-Sen UniversityGuang ZhouChina
  4. 4.Department of Basic Biomedical SciencesMercer University School of MedicineSavannahUSA

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