Protein kinase C α and ε phosphorylation of troponin and myosin binding protein C reduce Ca2+ sensitivity in human myocardium
Previous studies indicated that the increase in protein kinase C (PKC)-mediated myofilament protein phosphorylation observed in failing myocardium might be detrimental for contractile function. This study was designed to reveal and compare the effects of PKCα- and PKCε-mediated phosphorylation on myofilament function in human myocardium. Isometric force was measured at different [Ca2+] in single permeabilized cardiomyocytes from failing human left ventricular tissue. Activated PKCα and PKCε equally reduced Ca2+ sensitivity in failing cardiomyocytes (ΔpCa50 = 0.08 ± 0.01). Both PKC isoforms increased phosphorylation of troponin I- (cTnI) and myosin binding protein C (cMyBP-C) in failing cardiomyocytes. Subsequent incubation of failing cardiomyocytes with the catalytic subunit of protein kinase A (PKA) resulted in a further reduction in Ca2+ sensitivity, indicating that the effects of both PKC isoforms were not caused by cross-phosphorylation of PKA sites. Both isozymes showed no effects on maximal force and only PKCα resulted in a modest significant reduction in passive force. Effects of PKCα were only minor in donor cardiomyocytes, presumably because of already saturated cTnI and cMyBP-C phosphorylation levels. Donor tissue could therefore be used as a tool to reveal the functional effects of troponin T (cTnT) phosphorylation by PKCα. Massive dephosphorylation of cTnT with alkaline phosphatase increased Ca2+ sensitivity. Subsequently, PKCα treatment of donor cardiomyocytes reduced Ca2+ sensitivity (ΔpCa50 = 0.08 ± 0.02) and solely increased phosphorylation of cTnT, but did not affect maximal and passive force. PKCα- and PKCε-mediated phosphorylation of cMyBP-C and cTnI as well as cTnT decrease myofilament Ca2+ sensitivity and may thereby reduce contractility and enhance relaxation of human myocardium.
KeywordsProtein kinase C Cardiac Heart failure Myofilament function Contractile proteins Phosphorylation
The protein kinase C (PKC) family consists of a number of different isozymes with different substrate specificities . In the failing human heart PKC activity and the expression of the Ca2+ dependent isoform PKCα are increased . PKCα has been shown to play an important role in the development of cardiac hypertrophy and heart failure [7, 12, 13]. PKCα directly phosphorylates regulatory myofilament proteins such as cardiac troponin I (cTnI) and cardiac troponin T (cTnT) . PKCα also phosphorylates protein phosphatase inhibitor 1, which alters the activity of protein phosphatase 1 [7, 31]. PKCε is another (Ca2+ independent) PKC isoform, which translocates upon activation to the myofilaments (and the sarcolemma) [10, 15, 16]. Several myofilament regulatory proteins [myosin binding protein C (cMyBP-C), cTnT, cTnI] serve as targets for PKCε [24, 40]. Moreover, transgenic mice overexpressing constitutively active PKCε slowly develop dilated cardiomyopathy with failure .
Studies in rodent myocardium indicated that cTnT and cTnI phosphorylation influence Ca2+ sensitivity and were involved in the PKC-mediated depression of the maximal force generating capacity [8, 18, 32, 33]. Using an in vitro motility assay, an association was observed between the increased PKC expression in failing human hearts and a reduction of the force exerted on thin filaments . Moreover, a recent study using two different animal models of congestive heart failure, in which PKCα expression and activation was considerably increased, provided evidence that augmented PKCα-induced myofilament protein phosphorylation contributes to myofilament dysfunction by a reduction of maximum force and of Ca2+ sensitivity .
The expression of the different PKC isoforms, with different substrate specificity, varies between rodents and humans and with different disease states [1, 19]. This may affect the basal phosphorylation status of the myocardium and thus influence the effects of the kinases on Ca2+ sensitivity and force development per se. Specific information on the impact of the different PKC isoforms on contractile function in human myocardium is lacking. Therefore, we investigated the impact of PKCα and PKCε in human tissue from donor and end-stage failing hearts obtained during transplant surgery.
Materials and methods
Human ventricular tissue
Characteristics from donors and patients
ACEI, Diu, β
ACEI, Amio, Diu, β
ACEI, Diu, β
ACEI, Diu, β
Cardiomyocytes were mechanically isolated, Triton X-100 permeabilized and mounted in the experimental set-up at a sarcomere length of 2.2 μm. Force measurements and maximal rate of force redevelopment measurements were performed as described previously [28, 35, 36, 37].
Briefly, a single cardiomyocyte was mounted between a force transducer and piezoelectric motor. After transferring the cardiomyocyte from relaxing solution (5.89 mM Na2ATP, 6.48 mM MgCl2, 40.76 mM propionic acid (Kprop), 100 mM Bis-2-aminoethanesulfonic acid (BES), 7.0 mM ethylene glycol tetra acetic acid (EGTA), 14.5 mM phosphocreatine disodium salt (CrP), pH 7.10 at 15°C, pCa 9) to activating solution (5.97 mM Na2ATP, 6.28 mM MgCl2, 40.64 mM Kprop, 100 mM BES, 7.0 mM CaEGTA, 14.5 mM CrP, pH 7.10 at 15°C, pCa 4.5), isometric force was measured. After reaching steady force, the cardiomyocyte was 20% reduced in length within 2 ms and restretched after 30 ms (slack test). During this slack test, force first dropped to zero and after the restretch quickly redeveloped to the original steady state level. A single exponential was fitted to estimate the rate constant of force redevelopment at maximal activation (Ktr-max). Calcium sensitivity (pCa50) was determined by measuring force in activating solutions with submaximal [Ca2+] (pCa values ranged from 5.0 to 6.0) obtained by appropriate mixing of the activating and relaxing solutions. The initial control force at maximal [Ca2+] (i.e., pCa 4.5) was used to calculate the maximal force per cross-sectional area. The force values at submaximal [Ca2+] were normalized to the interpolated control values .
After the initial force-pCa series, the myocyte was incubated for 60 min at 20°C in a Ca2+-containing solution (pCa 5.9) containing 6 mM dithiothreitol (DTT), 1 μM PMA (phorbol 12-myristate 13-acetate; Sigma), 50 nM calyculin A (calA, Sigma) without kinase (time control), with 10 μg/mL human recombinant PKCα (Sigma P1782; batch 93K0330) or 10 μg/mL human recombinant PKCε (Sigma P1164; batch 060K1890). CalA was added to the incubation buffer to prevent protein dephosphorylation during incubation. The concentrations of the active PKC isoforms were saturating as no further effect on cardiomyocyte function were seen when the concentration was doubled. A separate group of failing myocytes was incubated for 40 min at 20°C in relaxing solution (see above) containing 6 mM DTT and 100 U/mL of the catalytic subunit of protein kinase A (PKA, Sigma P2645; batch 35H9522). After the incubation the force-pCa series was repeated. A third force-pCa series was performed to study sequential effects, i.e., PKC isoforms after PKA or visa versa.
The effect of PKCα was also investigated upon pretreatment of failing and donor cardiomyocytes with alkaline phosphatase (AP, calf intestinal, New England Biolabs; 2,000 U/mL) in relaxing solution (pCa 9) containing 6 mM DTT and 50 μL/mL protease inhibitor cocktail (PIC, Sigma, P8340) for 60 min at 20°C. All force measurements were performed at 15°C.
Endogenous protein phosphorylation
To preserve the endogenous phosphorylation status, frozen tissue samples (~1 mg dry weight) were homogenized in liquid nitrogen and resuspended in 1 mL cold (−20°C) 10% trichloroacetic acid solution (TCA; dissolved in acetone containing 0.1% (w/v) DTT) . Tissue homogenates were kept for 1 h at −80°C and slowly heated to room temperature by stepwise increments in temperature as follows: 20 min at −20°C, 20 min at 4°C and 20 min at room temperature (20 ± 2°C). The homogenates were thoroughly mixed on a vortex between all steps. Thereafter, the tissue homogenates were centrifuged at 12,000g for 15 min and tissue pellets were washed with 1 mL of 0.2% (w/v) DTT-acetone solution and shaken for 5 min at room temperature. Centrifugation, washing and shaking was repeated three times. Thereafter tissue pellets were freeze-dried and homogenized in 1D-sample buffer containing 15% glycerol, 62.5 mM Tris (pH 6.8), 1% (w/v) SDS and 2% (w/v) DTT (final concentration 2.5 μg dry weight/μL). To determine basal phosphorylation status of myofilament proteins, myocardial samples (TCA treated 20 μg/lane) were separated on gradient gels (Criterion Tris–HCl 4–15% gel, BioRad) and stained with Pro-Q diamond phosphoprotein gel stain (Pro-Q; Molecular Probes) in conjunction with SYPRO Ruby staining (Molecular Probes) of the gels . The phosphorylation signals for myofilament proteins were normalized to the intensities of the SYPRO Ruby-stained myosin binding protein C (cMyBP-C) bands. All signals were quantified using the luminescent image analyzer LAS-3000 (Fuji Science Imaging Systems) and Aida image analyzer software (Isotopenmeβgeräte GmbH, Staubenhardt, Germany).
Alterations in myofilament protein phosphorylation
To investigate the effect of the PKC isoforms as well as of PKA and AP on myofilament protein phosphorylation 600 μg (dry weight) of tissue was treated in with Triton X-100 (0.5%) in relaxing solution (pCa 9) containing PIC (5 μL/mL), phosphatase inhibitor cocktail (PhIC2, Sigma, P5726, 5 μL/mL) and calA (50 nM) for 5 min at room temperature, washed twice with relaxing solution without Triton X-100 and subsequently incubated for 60 min (or 40 min for PKA) at 20°C in 100 μL of (1) relaxing solution containing PIC (50 μL/mL), PhIC2 (50 μL/mL), calA (50 nM) and DTT (6 mM)(=control incubation); (2) activating solution (pCa 5.9) containing PIC (50 μL/mL), PhIC2 (50 μL/mL), calA (50 nM), DTT (6 mM), PMA (1 μM) and PKCα (10 μg/mL) or (3) PKCε (10 μg/mL); (4) relaxing solution containing PIC (50 μL/mL), PhIC2 (50 μL/mL), calA (50 nM), DTT (6 mML) and PKA (100 U/mL); (5) relaxing solution containing PIC (50 μL/mL), DTT (6 mM) and AP (2,000 U/mL). Part of the tissue treated with AP was washed and subsequently incubated with PKCα (see incubation no. 2). Subsequently, tissue was treated with TCA and analyzed using ProQ-stained gradient gels as described above.
To investigate the effect of PKCα phosphorylation of titin, tissue samples with a final concentration of ~10 μg/μL dry weight were homogenized in 100-150 μL sample buffer consisting of 0.55 M Tris HCL, 0.55 M EDTA, 10% glycerol, 1% β-mercaptoethanol, 20% (w/v) SDS, 8 μL/mL leupeptin (Peptin-institute, Japan), 10 μL/mL PhIC1 (Sigma, P2850) and PhIC2, pH6.8. Myocardial samples (~100 μg/lane) were separated on agarose strengthened 2% sodium dodecyl sulfate-polyacrylamide gels and stained with Pro-Q diamond phosphoprotein gel stain in conjunction with Sypro Ruby staining . Staining and analysis as described above.
Force-pCa relations were fit to the Hill equation as described previously . Values are given as mean ± SEM of n experiments. Cardiomyocyte force values for donor and failing samples were compared using an unpaired Student t test. Effects of incubations without or with PKCα, PKCε, PKA or AP were tested with paired Student t test. Repeated measures ANOVA followed by a Bonferroni post hoc test was used when studying the effects of sequential incubations with the PKC isoforms, PKA or AP. P < 0.05 was considered significant.
PKCα and PKCε phosphorylation of cTnI and cMyBP-C decrease Ca2+ sensitivity in failing myocardium
Overview of the force measurements with PKCα, PKCε and PKA incubations (a) and AP and PKCα incubations (b)
7 failing hearts; 14 myocytes
28.2 ± 2.4
1.5 ± 0.2
5.67 ± 0.03
3.43 ± 0.18
0.69 ± 0.03
28.6 ± 2.4
1.4 ± 0.2*
5.59 ± 0.03*
3.28 ± 0.24
0.81 ± 0.04*
5 failing hearts; 7 myocytes
30.3 ± 3.9
1.6 ± 0.3
5.68 ± 0.03
3.86 ± 0.20
0.60 ± 0.05
31.2 ± 3.1
1.4 ± 0.2
5.60 ± 0.02*
3.42 ± 0.13
0.74 ± 0.05*
7 failing hearts; 14 myocytes
22.8 ± 2.1
1.4 ± 0.2
5.65 ± 0.02
3.34 ± 0.07
0.68 ± 0.02
22.9 ± 2.0
1.4 ± 0.1
5.53 ± 0.01*
3.57 ± 0.13*
0.84 ± 0.04*
2 failing hearts; 6 myocytes
22.7 ± 2.5
1.5 ± 0.1
5.66 ± 0.03
3.16 ± 0.12
0.64 ± 0.06
22.1 ± 2.5
1.3 ± 0.1
5.67 ± 0.03
2.65 ± 0.11*
0.74 ± 0.04
5 failing hearts; 11 myocytes
35.7 ± 3.6
1.8 ± 0.3
5.64 ± 0.02
3.68 ± 0.11
0.76 ± 0.07
38.6 ± 4.5*
2.5 ± 0.3*
5.73 ± 0.03*
2.85 ± 0.13*
0.68 ± 0.04
AP + PKCα
5 failing hearts; 10 myocytes
35.5 ± 3.8
2.6 ± 0.3
5.73 ± 0.03
2.84 ± 0.14
0.56 ± 0.06
35.1 ± 3.9
2.4 ± 0.4
5.60 ± 0.01*
2.99 ± 0.15
0.66 ± 0.09
4 donor hearts; 8 myocytes
34.1 ± 4.5
1.5 ± 0.2
5.48 ± 0.02
4.04 ± 0.19
0.62 ± 0.09
34.3 ± 4.9
2.0 ± 0.4*
5.57 ± 0.02*
3.33 ± 0.18*
0.67 ± 0.07
AP + PKCα
4 donor hearts; 7 myocytes
31.6 ± 4.4
2.2 ± 0.4
5.59 ± 0.02
3.25 ± 0.18
0.57 ± 0.04
31.3 ± 4.8
2.0 ± 0.4
5.51 ± 0.02*
3.30 ± 0.15
0.76 ± 0.07*
Figure 1c shows a phosphoprotein (ProQ) stained 1D gel of failing tissue samples treated with PKCα and PKCε, at concentrations of 10 and 50 μg/mL. The phosphorylated active forms of PKCα and PKCε became apparent in the incubations with 50 μg/mL kinase. Gels were subsequently stained with SYPRO Ruby to correct for differences in protein loading. The phosphorylation levels with 50 μg/mL kinase were similar to the incubations with 10 μg/mL kinase, indicating that the standard concentrations of 10 μg/mL kinase yielded the maximum obtainable phosphorylation level. To further investigate if PKCα and PKCε are able to phosphorylate cTn, recombinant human troponin complex  was incubated with both isoforms. The results on the recombinant, non-phosphorylated troponin complex clearly indicate that PKCα and PKCε both phosphorylate cTnT and cTnI and that the efficacy of PKCε was less than that of PKCα (Fig. 1d).
On average, Ktr-max [tension redevelopment rate (s−1) at maximal Ca2+ concentration] was significantly increased after PKCα, PKCε and PKA incubation in failing cardiomyocytes (Table 2a). However, it must be noted that the change in Ktr-max in failing cells upon kinase treatments did not significantly differ from the increase observed in the (time) control incubations (Table 2a). These results suggest that the kinases and phosphatase used have little, if any, effect on the kinetics of force redevelopment at saturating calcium concentration.
PKCα decreases Ca2+ sensitivity via cTnT phosphorylation
This study shows that PKC-mediated phosphorylation of cMyBP-C and cTnI as well as of cTnT cause a reduction of Ca2+ sensitivity of force development in human myocardium. No effects on maximal force generating capacity of the myofilaments were observed, neither upon direct PKC treatment nor following phosphatase pretreatment. On the basis of these data we propose that increased PKC activity in failing myocardium may depress systolic function of the heart as for the same intracellular Ca2+ concentration, the myofilament force generation and thereby developed pressure will be reduced. On the other hand, the reduced myofilament Ca2+ sensitivity may help myocardial relaxation as Ca2+ concentrations are increased in failing hearts [3, 9].
PKCα and PKCε phosphorylation of cTnI and cMyBP-C decrease Ca2+ sensitivity in failing myocardium
Direct incubation of the cardiomyocytes from failing hearts with saturating amounts of active PKCα and PKCε resulted in a decrease in Ca2+ sensitivity (Fig. 1). PKCα and PKCε incubations of failing tissue resulted in increased phosphorylation of cMyBP-C and cTnI. PKCα incubation resulted in higher phosphorylation of cTnI and PKCε in higher phosphorylation levels of cMyBP-C, indicating isozyme specific substrate affinity. Although phosphorylation effects were diverse, the functional consequences of both PKC isoforms were quite similar.
It has previously been determined that cMyBP-C has PKC-phosphorylatable residues [24, 40]. However, so far no data has been published (to our knowledge) that directly link cMyBP-C phosphorylation with alterations in Ca2+ sensitivity. Our data suggest involvement of PKC-mediated cMyBP-C phosphorylation in the reduced Ca2+ sensitivity observed in failing tissue.
Kobayashi et al.  indicated that PKCε was able to phosphorylate PKA sites on mouse cTnI. Our previous study also showed that the active catalytic domain of PKC caused cross-phosphorylation of the PKA sites (Ser23/24) . We addressed this issue for PKCα- and PKCε-mediated phosphorylation by performing experiments where incubations of PKCα or PKCε were followed by PKA as well as by incubations in the reverse order. The results showed that the shifts in Ca2+ sensitivity in failing myocardium did not depend on the sequence of application of PKA and PKCα/ε. This indicates that the effects of the PKC isoforms and PKA were independent and that the PKCα/ε-mediated effects were not caused by cross-phosphorylation of the PKA sites under our conditions. Kobayashi et al.  used the recombinant mouse cTnI and an excess of PKCε, therefore it is possible that the cross-phosphorylation they observed does not occur under more physiological conditions or that it is more pronounced in mice than in human cTn.
PKCα decreases Ca2+ sensitivity via cTnT phosphorylation
Direct incubation of donor cells with PKCα did not alter Ca2+ sensitivity (Fig. 4b), which is in line with minor effects of PKCα on protein phosphorylation of cMyBP-C, desmin, cTnI, cTnT and MLC2 (Fig. 4c). The absence of functional effects of PKCα is most likely explained by the relatively high baseline phosphorylation status of cMyBP-C, cTnI and cTnT in donor myocardium. Because endogenous cMyBP-C and cTnI phosphorylation levels in donor tissue were high (Fig. 4a) and no major effects of PKCα on Ca2+ sensitivity and protein phosphorylation were observed (Fig. 4b, c), the donor tissue was used as a tool to reveal functional effects of PKCα-mediated phosphorylation of cTnT.
AP treatment resulted in a massive decrease in cTnT phosphorylation in failing and donor myocardium. Subsequent treatment with PKCα solely increased phosphorylation of cTnT in donor myocardium, which made it possible to identify the role of cTnT upon phosphorylation by PKCα. Treatment with PKCα following AP treatment showed a significant reduction in Ca2+ sensitivity in both failing and donor tissue. These results indicate that AP is able to dephosphorylate sites on cTnT, which are subsequently targeted by PKCα and when phosphorylated reduce Ca2+ sensitivity.
Overall, our results are in agreement with previous animal studies [17, 24, 40] indicating that functionally relevant targets of PKCα- and PKCε-mediated Ca2+ desensitization of the myofilaments are located on cardiac troponin. Both cTnI and cTnT contain a number of different PKC-phosphorylatable sites. On the basis of biochemical in vitro studies Ser-42 and Ser-44 and Thr-143 on cTnI  and Thr-194, Ser-198, Thr-203 and Thr-284 on cTnT [17, 34] are considered the main sites (phosphorylated sites based on human sequence). It has been proposed that the relative balance of phosphorylation of the three PKC sites on cTnI is important for the regulation of its function . Pseudophosphorylation of Ser-42/Ser-44 significantly reduced the myofilament response to calcium and phosphorylation of Thr-143 induced an increase in sensitivity to calcium [7, 39]. Among the four phosphorylation sites on cTnT, Thr-203 appeared to be the most important for modulation of cTn function in that it inhibited force as well as its Ca2+ sensitivity .
PKCα reduces passive force, but not maximal force
Rodent studies indicate that PKC-mediated phosphorylation results in a reduction of the maximum force generating capacity of the cardiomyocytes [8, 26, 32, 33]. A study using an in vitro motility assay suggested that this might also be the case in human tissue . However, the present results did not show depression of force upon PKC treatment and indicate that caution should be exerted when extrapolating data obtained in rodent studies to force development in human cardiomyocytes. Cardiac cTnI and cTnT phosphorylation by PKC may occur at different sites in different species and may explain the absence of an effect of PKCα on Fmax in human cardiomyocytes. Only recently, using mass spectrometry Zabrouskov et al.  revealed a novel phosphorylation site (Ser-76 or Thr-77) in human cTnI, while no phosphorylation was observed at the sites mentioned above. A study in mice revealed that the PKCα-induced reduction in Fmax critically depended on cTnT phosphorylation at Thr-203 . Future studies should be performed to reveal which sites are phosphorylated by PKC in human myocardium. Recently Molnár et al.  showed that PKCα contributes to the maintenance of contractile force in human cardiomyocytes, which is in line with our results that PKCα treatment does not negatively affect maximal force. AP treatment resulted in a small (~5%) but significant increase in Fmax. However, subsequent treatment with PKCα did not result in a reduction of force development. This suggests that AP is able to dephosphorylate at least to some extent one of the contractile proteins determining Fmax. However, in human tissue the impact is rather small and the link with PKCα is not evident.
PKCα treatment resulted in a small reduction of Fpas both in donor and in failing cardiomyocytes, whereas PKCε had no significant effect but did show a similar trend (Table 2). AP treatment caused a significant increase in Fpas in both failing and donor cells but subsequent PKCα treatment did not restore the original levels. These results suggest that the effects of PKCα-mediated phosphorylation and AP-induced dephosphorylation on stiffness are independent, and indicate that at least two distinct phosphorylation sites, possibly on titin [4, 22, 41], are involved in the modulation of passive stiffness of the cardiomyocytes. To address this issue, the effect of PKCα in titin phosphorylation after incubation with PKCα was investigated both in donor and failing tissue (Fig. 2). However, no significant effect was observed. This could imply that phosphorylation of titin is not solely responsible for the decrease in passive force observed after PKCα. However, since titin may contain numerous phosphorylation sites with one or few PKCα dependent sites, it might be that our method is not sensitive enough to resolve the minor relative changes in titin phosphorylation as appeared to be the case for protein kinase G by Krüger et al. .
As high Fpas has been associated with high left ventricular end-diastolic pressure in patients with diastolic dysfunction , the reduction in passive stiffness by PKCα-mediated phosphorylation might improve diastolic filling of the heart.
Limitations and conclusions
In this study the effects of the kinases and phosphatases on the phosphorylation levels are represented relative to basal phosphorylation levels for each protein. Although the changes in protein phosphorylation levels were relatively small, the functional changes were significant and may be physiologically relevant.
Since the transition to end-stage heart failure is accompanied by an increase in PKCα activity  it is surprising that the effects of PKCα are observed only in end-stage failing hearts and become visible in donor hearts only after dephosphorylation by alkaline phosphatase. It should be noted, however, that the endogenous phosphorylation levels in the explanted myocardium studied not necessarily reflect the in vivo situation. The lower levels of cMyBP-C and cTnI phosphorylation observed in end-stage failing compared to donor myocardium may reflect desensitisation of the β-adrenergic pathway. Moreover, it has been suggested that high catecholamine levels at the time of tissue procurement underlies the relative high levels of cMyBP-C and cTnI phosphorylation levels in donor myocardium . Future studies in biopsy samples taken under well-controlled hemodynamic conditions with parallel measurements of blood catecholamines should be performed to address this issue. Nevertheless, the data presented in this study clearly indicate that changes in expression and activation of PKCα and PKCε in response to pathophysiological stressors or increased hemodynamic demands during exercise translate into alterations in contractile function in human myocardium.
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
- 5.Borbély A, Falcao-Pires I, van Heerebeek L, Hamdani N, Édes I, Gavina C, Leite-Moreira AF, Bronzwaer JGF, Papp Z, van der Velden J, Stienen GJM, Paulus WJ (2009) Hypophosphorylation of the stiff N2B titin isoform raises cardiomyocyte resting tension in failing human myocardium. Circ Res 104:780–786CrossRefPubMedGoogle Scholar
- 6.Bowling N, Walsh RA, Song G, Estridge T, Sandusky GE, Fouts RL, Mintze K, Pickard T, Roden R, Bristow MR, Sabbah HN, Mizrahi JL, Gromo G, King GL, Vlahos CJ (1999) Increased protein kinase C activity and expression of Ca2+-sensitive isoforms in the failing human heart. Circulation 99:384–391PubMedGoogle Scholar
- 7.Braz JC, Gregory K, Pathak A, Zhao W, Sahin B, Klevitsky R, Kimball TF, Lorenz JN, Nairn AC, Liggett SB, Bodi I, Wang S, Schwartz A, Lakatta EG, DePaoli-Roach AA, Robbins J, Hewett TE, Bibb JA, Westfall MV, Kranias EG, Molkentin JD (2004) PKC-α regulates cardiac contractility and propensity toward heart failure. Nature 10:248–254CrossRefGoogle Scholar
- 8.Burkart EM, Sumandea MP, Kobayashi T, Nili M, Martin AF, Homsher E, Solaro RJ (2003) Phosphorylation or glutamic acid substitution at protein kinase C sites on cardiac troponin I differentially depress myofilament tension and shortening velocity. J Biol Chem 278:11265–11272CrossRefPubMedGoogle Scholar
- 13.Hambleton M, Hahn H, Pleger ST, Kuhn MC, Klevitsky R, Carr AN, Kimball TF, Hewett TE, Dorn GW 2nd, Koch WJ, Molkentin JD (2006) Pharmacological- and gene therapy-based inhibition of protein kinase C alpha/beta enhances cardiac contractility and attenuates heart failure. Circulation 114:574–582CrossRefPubMedGoogle Scholar
- 17.Jideama NM, Noland TA Jr, Raynor RL, Blobe GC, Fabbro D, Kazanietz MG, Blumberg PM, Hannun YA, Kuo JF (1996) Phosphorylation specificities of protein kinase C isozymes for bovine cardiac troponin I and troponin T and sites within these proteins and regulation of myofilament properties. J Biol Chem 271:23277–23283CrossRefPubMedGoogle Scholar
- 20.Kobayashi T, Yang X, Walker LA, Van Breemen RB, Solaro RJ (2005) A non-equilibrium isoelectric focusing method to determine states of phosphorylation of cardiac troponin I: identification of Ser-23 and Ser-24 as significant sites of phosphorylation by protein kinase C. J Mol Cell Cardiol 38:213–218CrossRefPubMedGoogle Scholar
- 25.Molnár A, Borbély A, Czuriga D, Ivetta SM, Szilágyi S, Hertelendi Z, Pásztor ET, Galajda Z, Szerafin T, Jaquet K, Papp Z, Édes I, Tóth A (2008) Protein kinase C contributes to the maintenance of contractile force in human ventricular cardiomyocytes. J Biol Chem 284:1031–1039CrossRefPubMedGoogle Scholar
- 28.Narolska NA, Piroddi N, Belus A, Boontje NM, Scellini B, Deppermann S, Zaremba R, Musters RJ, dos Remedios C, Jaquet K, Foster DB, Murphy AM, van Eyk JE, Tesi C, Poggesi C, van der Velden J, Stienen GJ (2006) Impaired diastolic function after exchange of endogenous troponin I with C-terminal truncated troponin I in human cardiac muscle. Circ Res 99:1012–1020CrossRefPubMedGoogle Scholar
- 31.Pathak A, del Monte F, Zhao W, Schultz JE, Lorenz JN, Bodi I, Weiser D, Hahn H, Carr AN, Syed F, Mavila N, Jha L, Qian J, Marreez Y, Chen G, McGraw DW, Heist EK, Guerrero JL, DePaoli-Roach AA, Hajjar RJ, Kranias EG (2005) Enhancement of cardiac function and suppression of heart failure progression by inhibition of protein phosphatase1. Circ Res 96:756–766CrossRefPubMedGoogle Scholar
- 37.Van der Velden J, Papp Z, Zaremba R, Boontje NM, de Jong JW, Owen VJ, Burton PB, Goldmann P, Jaquet K, Stienen GJM (2003) Increased Ca2+-sensitivity of the contractile apparatus in end-stage human heart failure results from altered phosphorylation of contractile proteins. Cardiovasc Res 57:37–47CrossRefPubMedGoogle Scholar