Basic Research in Cardiology

, Volume 103, Issue 6, pp 514–524

TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

  • Ahmed Abdel-Latif
  • Ewa K. Zuba-Surma
  • Jamie Case
  • Sumit Tiwari
  • Greg Hunt
  • Smita Ranjan
  • Robert J. Vincent
  • Edward F. Srour
  • Roberto Bolli
  • Buddhadeb Dawn
ORIGINAL CONTRIBUTION

DOI: 10.1007/s00395-008-0729-9

Cite this article as:
Abdel-Latif, A., Zuba-Surma, E.K., Case, J. et al. Basic Res Cardiol (2008) 103: 514. doi:10.1007/s00395-008-0729-9

Abstract

The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

Key words

adult stem cell cardiac differentiation culture medium skeletal muscle growth factor 

Supplementary material

395_2008_729_MOESM1_ESM.doc (20 kb)
ESM 1 (DOC 21 kb)
395_2008_729_MOESM2_ESM.avi (12.2 mb)
ESM 2 (AVI 12,481 kb)

Copyright information

© Springer 2008

Authors and Affiliations

  • Ahmed Abdel-Latif
    • 1
  • Ewa K. Zuba-Surma
    • 1
  • Jamie Case
    • 2
  • Sumit Tiwari
    • 1
  • Greg Hunt
    • 1
  • Smita Ranjan
    • 1
  • Robert J. Vincent
    • 1
  • Edward F. Srour
    • 3
  • Roberto Bolli
    • 1
  • Buddhadeb Dawn
    • 1
  1. 1.Division of CardiologyUniversity of LouisvilleLouisvilleUSA
  2. 2.Dept. of Medicine, Herman B Wells Center for Pediatric ResearchIndiana University School of MedicineIndianapolisUSA
  3. 3.Dept. of Microbiology/Immunology, Herman B Wells Center for Pediatric ResearchIndiana University School of MedicineIndianapolisUSA

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