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Basic Research in Cardiology

, Volume 101, Issue 6, pp 471–478 | Cite as

Increased expression and function of the myocardial Na–K–2Cl cotransporter in failing rat hearts

  • Geir Øystein AndersenEmail author
  • Erik Øie
  • Leif Erik Vinge
  • Arne Yndestad
  • Håvard Attramadal
  • Tor Skomedal
  • Jan Bjørn Osnes
ORIGINAL CONTRIBUTION

Abstract

Recent studies indicate a role of the Na–K–2Cl cotransporter (NKCC) in regulation of myocardial function. However, potential pathophysiological properties of NKCC in conditions like myocardial infarction (MI) and heart failure have not been explored. We investigated the cellular localization of myocardial NKCC and whether myocardial NKCC levels are changed upon induction of post-infarction heart failure in rats. Immunohistochemical analysis demonstrated extensive distribution of NKCC in normal rat myocardium with fairly strong expression in cardiomyocytes, fibroblasts, vascular endothelial cells, as well as smooth muscle cells. Myocardial mRNA levels of NKCC were investigated at 2, 7 and 28 days after induction of MI or sham operation, but no changes were found. Cardiomyocytes and non-cardiomyocytes were isolated 7 days after induction of MI or sham operation. An ∼2-fold increase of the NKCC mRNA levels was found in isolated cardiomyocytes from heart failure rats compared to that of sham-operated rats (P < 0.001), whereas a trend towards decreased mRNA levels of NKCC in isolated non-cardiomyocytes was observed. In addition, we found a bumetanide sensitive 86Rb+ influx mechanism present in the hearts after induction of MI (P < 0.05). Thus, our data indicate cardiomyocyte specific increase in NKCC mRNA levels and increased NKCC activity in post-infarction heart failure. Our results may indicate a potential role of NKCC during post-infarction remodeling.

Key words

Na–K–2Cl cotransporter bumetanide congestive heart failure 86Rb+ influx cardiomyocytes 

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Copyright information

© Steinkopff-Verlag 2006

Authors and Affiliations

  • Geir Øystein Andersen
    • 1
    • 2
    • 7
    Email author
  • Erik Øie
    • 2
    • 3
    • 5
  • Leif Erik Vinge
    • 2
    • 3
    • 5
  • Arne Yndestad
    • 2
    • 4
  • Håvard Attramadal
    • 2
    • 3
    • 6
  • Tor Skomedal
    • 1
    • 2
  • Jan Bjørn Osnes
    • 1
    • 2
  1. 1.Department of PharmacologyUniversity of OsloOsloNorway
  2. 2.Center for Heart Failure ResearchUniversity of OsloOsloNorway
  3. 3.Institute for Surgical ResearchUniversity of OslaOslaNorway
  4. 4.Research Institute for Internal MedicineUniversity of OsloOsloNorway
  5. 5.Medical DepartmentDiakonhjemmet HospitalOsloNorway
  6. 6.Department of CardiologyRikshospitalet University HospitalOsloNorway
  7. 7.Department of Cardiology, Heart and Lung CenterUllevaal University HospitalOsloNorway

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