Obesity, a strong risk factor for metabolic disorder, has become a major impediment for public health globally. The objective of this study was to assess the anti-obesity effect of mung bean, and the relationship between the gut microbiota modulatory effects of mung bean and the prevention of obesity.
Thirty-two four-week-old male C57BL/6 J mice were divided into four groups: normal chow diet (NCD), high-fat diet (HFD), a high-fat diet supplemented with 30% whole mung bean flour (HFD-WMB), and a high-fat diet supplemented with 30% decorticated mung bean flour (HFD-DMB). The ability of a mung bean-based diet to combat obesity-related metabolic disorder was determined by assessing the changes in physiological, histological, biochemical parameters, and gut microbiota composition of mice with HFD-induced obesity at 12 weeks.
Both of WMB and DMB supplementation can effectively alleviate HFD-induced lipid metabolic disorders, which was accompanied by a reduction in hepatic steatosis. However, the only supplementation with WMB significantly reduced HFD-induced body weight gain, fat accumulation, and adipocyte size, and ameliorated the glucose tolerance and insulin resistance by sensitizing insulin action. Furthermore, high-throughput pyrosequencing of 16S rRNA revealed that WMB and DMB supplementation could normalize HFD-induced gut microbiota dysbiosis. Especially, WMB and DMB supplementation significantly promoted the relative abundance of Akkermansia and Bifidobacterium, respectively, and both of them significantly restored the relative abundance of several HFD-dependent taxa back to normal status in this study. Spearman’s correlation analysis revealed that those genera are closely correlated with obesity-related indices.
Although WMB showed better beneficial effects on HFD-induced obesity in comparison with DMB, DMB still retained some health benefits. Moreover, the alleviation of HFD-induced changes by mung bean supplementation was, at least, partially conciliated by structural modulation of gut microbiota.
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Medina-Remón A, Kirwan R, Lamuela-Raventós RM, Estruch R (2018) Dietary patterns and the risk of obesity, type 2 diabetes mellitus, cardiovascular diseases, asthma, and neurodegenerative diseases. Crit Rev Food Sci Nutr 58(2):262–296. https://doi.org/10.1080/10408398.2016.1158690
Heymsfield SB, Wadden TA (2017) Mechanisms, pathophysiology, and management of obesity. N Engl J Med 376(3):254–266. https://doi.org/10.1056/NEJMra1514009
Ley RE, Turnbaugh PJ, Klein S, Gordon JI (2006) Human gut microbes associated with obesity. Nature 444(7122):1022–1023. https://doi.org/10.1038/4441022a
Tremaroli V, Bäckhed F (2012) Functional interactions between the gut microbiota and host metabolism. Nature 489:242. https://doi.org/10.1038/nature11552
Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, Sogin ML, Jones WJ, Roe BA, Affourtit JP, Egholm M, Henrissat B, Heath AC, Knight R, Gordon JI (2008) A core gut microbiome in obese and lean twins. Nature 457:480. https://doi.org/10.1038/nature07540 https://www.nature.com/articles/nature07540#supplementary-information
Gong L, Cao W, Chi H, Wang J, Zhang H, Liu J, Sun B (2018) Whole cereal grains and potential health effects: Involvement of the gut microbiota. Food Res Int 103:84–102. https://doi.org/10.1016/j.foodres.2017.10.025
Rowland I, Gibson G, Heinken A, Scott K, Swann J, Thiele I, Tuohy K (2018) Gut microbiota functions: metabolism of nutrients and other food components. Eur J Nutr 57(1):1–24. https://doi.org/10.1007/s00394-017-1445-8
Marinangeli CPF, Jones PJH (2012) Pulse grain consumption and obesity: effects on energy expenditure, substrate oxidation, body composition, fat deposition and satiety. Br J Nutr 108(S1):S46–S51. https://doi.org/10.1017/S0007114512000773
Rebello CJ, Greenway FL, Finley JW (2014) Whole grains and pulses: a comparison of the nutritional and health benefits. J Agric Food Chem 62(29):7029–7049. https://doi.org/10.1021/jf500932z
Hou D, Yousaf L, Xue Y, Hu J, Wu J, Hu X, Feng N, Shen Q (2019) Mung bean (Vigna radiata L.) bioactive polyphenols, polysaccharides, peptides, and health benefits. Nutrients 11(6):1238. https://doi.org/10.3390/nu11061238
Yao Y, Chen F, Wang M, Wang J, Ren G (2008) Antidiabetic activity of mung bean extracts in diabetic KK-Ay mice. J Agric Food Chem 56(19):8869–8873. https://doi.org/10.1021/jf8009238
Inhae K, Seojin C, Joung HT, Munji C, Hae-Ri W, Won LB, Myoungsook L (2015) Effects of mung bean (Vigna radiata L.) ethanol extracts decrease proinflammatory cytokine-induced lipogenesis in the KK-Ay diabese mouse model. J Med Food 18(8):841–849. https://doi.org/10.1089/jmf.2014.3364
Xie J, Du M, Shen M, Wu T, Lin L (2019) Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from mung bean (Vigna radiate). Food Chem 270:243–250. https://doi.org/10.1016/j.foodchem.2018.07.103
Joghatai M, Barari L, Mousavie Anijdan SH, Elmi MM (2018) The evaluation of radio-sensitivity of mung bean proteins aqueous extract on MCF-7, hela and fibroblast cell line. Int J Radiat Biol 94(5):478–487. https://doi.org/10.1080/09553002.2018.1446226
Lopes LAR, Martins MDCC, Farias LM, Brito AKS, Lima GDM, Carvalho VBL, Pereira CFC, Conde Júnior AM, Saldanha T, Arêas JAG, Silva KJD, Frota KDMG (2018) Cholesterol-lowering and liver-protective effects of cooked and germinated mung beans (Vigna radiata L.). Nutrients 10(7):821. https://doi.org/10.3390/nu10070821
Dai Z, Su D, Zhang Y, Sun Y, Hu B, Ye H, Jabbar S, Zeng X (2014) Immunomodulatory activity in vitro and in vivo of verbascose from mung beans (Phaseolus aureus). J Agric Food Chem 62(44):10727–10735. https://doi.org/10.1021/jf503510h
Mubarak AE (2005) Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem 89(4):489–495. https://doi.org/10.1016/j.foodchem.2004.01.007
Andersson KE, Chawade A, Thuresson N, Rascon A, Öste R, Sterner O, Olsson O, Hellstrand P (2017) Wholegrain oat diet changes the expression of genes associated with intestinal bile acid transport. Mol Nutr Food Res 61(7):1600874. https://doi.org/10.1002/mnfr.201600874
Liyanage R, Kiramage C, Visvanathan R, Jayathilake C, Weththasinghe P, Bangamuwage R, Chaminda Jayawardana B, Vidanarachchi J (2018) Hypolipidemic and hypoglycemic potential of raw, boiled, and sprouted mung beans (Vigna radiata L. Wilczek) in rats. J Food Biochem 42(1):e12457. https://doi.org/10.1111/jfbc.12457
Hou D, Zhao Q, Yousaf L, Khan J, Xue Y, Shen Q (2020) Consumption of mung bean (Vigna radiata L.) attenuates obesity, ameliorates lipid metabolic disorders and modifies the gut microbiota composition in mice fed a high-fat diet. J Funct Foods 64:103687. https://doi.org/10.1016/j.jff.2019.103687
Sarma SM, Khare P, Jagtap S, Singh DP, Baboota RK, Podili K, Boparai RK, Kaur J, Bhutani KK, Bishnoi M, Kondepudi KK (2017) Kodo millet whole grain and bran supplementation prevents high-fat diet induced derangements in a lipid profile, inflammatory status and gut bacteria in mice. Food Funct 8(3):1174–1183. https://doi.org/10.1039/C6FO01467D
Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO (2012) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41(D1):D590–D596. https://doi.org/10.1093/nar/gks1219
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5):335–336. https://doi.org/10.1038/nmeth.f.303
Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C (2011) Metagenomic biomarker discovery and explanation. Genome Biol 12(6):R60. https://doi.org/10.1186/gb-2011-12-6-r60
Langille MGI, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, Beiko RG, Huttenhower C (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31(9):814–821. https://doi.org/10.1038/nbt.2676
Yao Y, Zhu Y, Ren G (2014) Mung bean protein increases plasma cholesterol by up-regulation of hepatic HMG-CoA reductase, and CYP7A1 in mRNA Levels. J Food Nutr Res 2(11):770–775. https://doi.org/10.12691/jfnr-2-11-2
Nakatani A, Li X, Miyamoto J, Igarashi M, Watanabe H, Sutou A, Watanabe K, Motoyama T, Tachibana N, Kohno M, Inoue H, Kimura I (2018) Dietary mung bean protein reduces high-fat diet-induced weight gain by modulating host bile acid metabolism in a gut microbiota-dependent manner. Biochem Biophys Res Commun 501(4):955–961. https://doi.org/10.1016/j.bbrc.2018.05.090
Luo J, Cai W, Wu T, Xu B (2016) Phytochemical distribution in hull and cotyledon of adzuki bean (Vigna angularis L.) and mung bean (Vigna radiate L.), and their contribution to antioxidant, anti-inflammatory and anti-diabetic activities. Food Chem 201:350–360. https://doi.org/10.1016/j.foodchem.2016.01.101
Zhong L, Fang Z, Wahlqvist ML, Wu G, Hodgson JM, Johnson SK (2018) Seed coats of pulses as a food ingredient: characterization, processing, and applications. Trends Food Sci Technol 80:35–42. https://doi.org/10.1016/j.tifs.2018.07.021
Jang Y-H, Kang M-J, Choe E-O, Shin M, Kim J-I (2014) Mung bean coat ameliorates hyperglycemia and the antioxidant status in type 2 diabetic db/db mice. Food Sci Biotechnol 23(1):247–252. https://doi.org/10.1007/s10068-014-0034-3
Kohno M, Motoyama T, Shigihara Y, Sakamoto M, Sugano H (2017) Improvement of glucose metabolism via mung bean protein consumption: a clinical trial of GLUCODIA TM isolated mung bean protein in Japan. Funct Foods Health Dis 7:115–134. https://doi.org/10.1017/jns.2017.68
Carmiel-Haggai M, Cederbaum AI, Nieto N (2005) A high-fat diet leads to the progression of non-alcoholic fatty liver disease in obese rats. FASEB J 19(1):136–138. https://doi.org/10.1096/fj.04-2291fje
Watanabe H, Inaba Y, Inoue H, Kimura K, Kaneko S, Asahara S-i, Kido Y, Matsumoto M, Kohno M, Tachibana N, Motoyama T (2016) Dietary mung bean protein reduces hepatic steatosis, fibrosis, and inflammation in male mice with diet-induced, nonalcoholic fatty liver disease. J Nutr 147(1):52–60. https://doi.org/10.3945/jn.116.231662
Liu T, Yu XH, Gao EZ, Liu XN, Sun LJ, Li HL, Wang P, Zhao YL, Yu ZG (2014) Hepatoprotective effect of active constituents isolated from mung beans (Phaseolus radiates L.) in an alcohol-induced liver injury mouse model. J Food Biochem 38(5):453–459. https://doi.org/10.1111/jfbc.12083
Viuda-Martos M, López-Marcos MC, Fernández-López J, Sendra E, López-Vargas JH, Pérez-Álvarez JA (2010) Role of fiber in cardiovascular diseases: a review. Compr Rev Food Sci Food Safety 9(2):240–258. https://doi.org/10.1111/j.1541-4337.2009.00102.x
Silva FM, Kramer CK, de Almeida JC, Steemburgo T, Gross JL, Azevedo MJ (2013) Fiber intake and glycemic control in patients with type 2 diabetes mellitus: a systematic review with meta-analysis of randomized controlled trials. Nutr Rev 71(12):790–801. https://doi.org/10.1111/nure.12076
Cho SS, Qi L, Fahey GC Jr, Klurfeld DM (2013) Consumption of cereal fiber, mixtures of whole grains and bran, and whole grains and risk reduction in type 2 diabetes, obesity, and cardiovascular disease. Am J Clin Nutr 98(2):594–619. https://doi.org/10.3945/ajcn.113.067629
Weickert MO, Pfeiffer AF (2018) Impact of dietary fiber consumption on insulin resistance and the prevention of type 2 diabetes. J Nutr 148(1):7–12. https://doi.org/10.1093/jn/nxx008
Delzenne NM, Cani PD (2011) Interaction between obesity and the gut microbiota: relevance in nutrition. Annu Rev Nutr 31(1):15–31. https://doi.org/10.1146/annurev-nutr-072610-145146
Delzenne NM, Neyrinck AM, Bäckhed F, Cani PD (2011) Targeting gut microbiota in obesity: effects of prebiotics and probiotics. Nature Rev Endocrinol 7(11):639–646. https://doi.org/10.1038/nrendo.2011.126
Laparra JM, Sanz Y (2010) Interactions of gut microbiota with functional food components and nutraceuticals. Pharmacol Res 61(3):219–225. https://doi.org/10.1016/j.phrs.2009.11.001
Conlon MA, Bird AR (2015) The impact of diet and lifestyle on gut microbiota and human health. Nutrients 7(1):17–44
Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI (2005) Obesity alters gut microbial ecology. Proc Natl Acad Sci USA 102(31):11070–11075. https://doi.org/10.1073/pnas.0504978102
Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI (2006) An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444(7122):1027–1031. https://doi.org/10.1038/nature05414
Nicholson JK, Holmes E, Kinross J, Burcelin R, Gibson G, Jia W, Pettersson S (2012) Host-gut microbiota metabolic interactions. Science 336(6086):1262–1267. https://doi.org/10.1126/science.1223813
Lee W-J, Hase K (2014) Gut microbiota–generated metabolites in animal health and disease. Nat Chem Biol 10(6):416–424. https://doi.org/10.1038/nchembio.1535
Jayachandran M, Chung SSM, Xu B (2019) A critical review of the relationship between dietary components, the gut microbe Akkermansia muciniphila, and human health. Crit Rev Food Sci Nutr. https://doi.org/10.1080/10408398.2019.1632789
Everard A, Belzer C, Geurts L, Ouwerkerk JP, Druart C, Bindels LB, Guiot Y, Derrien M, Muccioli GG, Delzenne NM, de Vos WM, Cani PD (2013) Cross-talk between %3cem%3eAkkermansia muciniphila%3c/em%3e and intestinal epithelium controls diet-induced obesity. Proc Natl Acad Sci 110(22):9066–9071. https://doi.org/10.1073/pnas.1219451110
Shin N-R, Lee J-C, Lee H-Y, Kim M-S, Whon TW, Lee M-S, Bae J-W (2014) An increase in the %3cem%3eAkkermansia%3c/em%3e spp. population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice. Gut 63(5):727–735. https://doi.org/10.1136/gutjnl-2012-303839
Liu S, Li F, Zhang X (2019) Structural modulation of gut microbiota reveals coix seed contributes to weight loss in mice. Appl Microbiol Biotechnol 103(13):5311–5321. https://doi.org/10.1007/s00253-019-09786-z
Li Y, Cui Y, Lu F, Wang X, Liao X, Hu X, Zhang Y (2019) Beneficial effects of a chlorophyll-rich spinach extract supplementation on prevention of obesity and modulation of gut microbiota in high-fat diet-fed mice. J Funct Foods 60:103436. https://doi.org/10.1016/j.jff.2019.103436
Million M, Maraninchi M, Henry M, Armougom F, Richet H, Carrieri P, Valero R, Raccah D, Vialettes B, Raoult D (2012) Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii. Int J Obes 36(6):817–825. https://doi.org/10.1038/ijo.2011.153
Chen J, Wang R, Li X-F, Wang R-L (2011) Bifidobacterium adolescentis supplementation ameliorates visceral fat accumulation and insulin sensitivity in an experimental model of the metabolic syndrome. Br J Nutr 107(10):1429–1434. https://doi.org/10.1017/S0007114511004491
Wang P, Li D, Ke W, Liang D, Hu X, Chen F (2019) Resveratrol-induced gut microbiota reduces obesity in high-fat diet-fed mice. Int J Obes. https://doi.org/10.1038/s41366-019-0332-1
Gan R-Y, Deng Z-Q, Yan A-X, Shah NP, Lui W-Y, Chan C-L, Corke H (2016) Pigmented edible bean coats as natural sources of polyphenols with antioxidant and antibacterial effects. LWT 73:168–177. https://doi.org/10.1016/j.lwt.2016.06.012
Duda-Chodak A, Tarko T, Satora P, Sroka P (2015) Interaction of dietary compounds, especially polyphenols, with the intestinal microbiota: a review. Eur J Nutr 54(3):325–341. https://doi.org/10.1007/s00394-015-0852-y
Ozdal T, Sela DA, Xiao JB, Boyacioglu D, Chen F, Capanoglu E (2016) The reciprocal interactions between polyphenols and gut microbiota and effects on bioaccessibility. Nutrients 8(2):36. https://doi.org/10.3390/nu8020078
Myint H, Kishi H, Iwahashi Y, Saburi W, Koike S, Kobayashi Y (2018) Functional modulation of caecal fermentation and microbiota in rat by feeding bean husk as a dietary fibre supplement. Benef Mirbobes 9(6):963–974. https://doi.org/10.3920/bm2017.0174
Yang L, Zhao Y, Huang J, Zhang H, Lin Q, Han L, Liu J, Wang J, Liu H (2019) Insoluble dietary fiber from soy hulls regulates the gut microbiota in vitro and increases the abundance of bifidobacteriales and lactobacillales. J Food Sci Technol. https://doi.org/10.1007/s13197-019-04041-9
Forgie AJ, Gao Y, Ju T, Pepin DM, Yang K, Gänzle MG, Ozga JA, Chan CB, Willing BP (2019) Pea polyphenolics and hydrolysis processing alter microbial community structure and early pathogen colonization in mice. J Nutr Biochem 67:101–110. https://doi.org/10.1016/j.jnutbio.2019.01.012
This work was financially supported by the National Key Research and Development Program of China (2017YFD0401202).
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Hou, D., Zhao, Q., Yousaf, L. et al. Whole mung bean (Vigna radiata L.) supplementation prevents high-fat diet-induced obesity and disorders in a lipid profile and modulates gut microbiota in mice. Eur J Nutr (2020). https://doi.org/10.1007/s00394-020-02196-2
- Whole mung bean
- Decorticated mung bean
- Lipid disorders
- Gut microbiota