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Microbial insight into dietary protein source affects intestinal function of pigs with intrauterine growth retardation

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Abstract

Purpose

Dietary protein, as important macronutrient, is vital for intestinal function and health status. We aimed to determine the effects of dietary protein source on growth performance and intestinal function of neonates with intrauterine growth retardation (IUGR) in a pig model.

Methods

Eighteen pairs of IUGR and normal birth weight (NBW) weaned pigs were allotted to be fed starter diet containing soybean protein concentrate (SPC) or spray-dried porcine plasma (SDPP) for 2 weeks. Growth performance, antioxidant variables, intestinal morphology and absorption capability, microbiota composition and short-chain fatty acids (SCFA) were assessed.

Results

IUGR led to poor growth performance, absorption capability and changes on antioxidant variables, while SDPP diet improved the growth performance, diarrhea index, intestinal morphology and antioxidant variables of IUGR or NBW pigs relative to that fed SPC diet. Importantly, SDPP diet improved bacterial diversity and increased the abundance of phylum Firmicutes, but decreased the phylum Proteobacteria in colonic digesta, associating with higher genera Lactobacillus and lower genera Escherichia–Shigella, linking to the increased concentration of SCFA.

Conclusions

Our findings indicate that IUGR impairs the growth rate, intestinal function and oxidative status of weaned pigs, which could be partly improved by SDPP diet either for IUGR or NBW pigs, associating with the better antioxidant capability, composition of microbiotas and their metabolites.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31101727); the International Cooperation in Science and Technology Project of Sichuan Province (2014HH0034); and the Natural Science Foundation of Sichuan Province (12ZA110).

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Author notes

  1. Lianqiang Che and Liang Hu contributed equally to this work.

Authors and Affiliations

  1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, Sichuan, China

    Lianqiang Che, Liang Hu, Qiang Zhou, Xie Peng, Yang Liu, Yuheng Luo, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, Jiayong Tang & De Wu

  2. Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ya’an, Sichuan, China

    Lianqiang Che, Liang Hu, Qiang Zhou, Xie Peng, Yang Liu, Yuheng Luo, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, Jiayong Tang & De Wu

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Contributions

The authors’ responsibilities were as follows: LQC, LH, DW and ZFF: designed the research; LH, QZ, XP, YL, JYT and YHL: conducted the research; YL, SYX, BF and JL: analyzed the data; LH: wrote the manuscript; LQC: had primary responsibility for the final contents; and all authors: read and approved the final manuscript.

Corresponding author

Correspondence to Lianqiang Che.

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The authors declare that they have no completing interests.

Ethical statement

The use of animals for this research was approved by the Institutional Animal Care and Use Committee of Sichuan Agricultural University.

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Che, L., Hu, L., Zhou, Q. et al. Microbial insight into dietary protein source affects intestinal function of pigs with intrauterine growth retardation. Eur J Nutr 59, 327–344 (2020). https://doi.org/10.1007/s00394-019-01910-z

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  • DOI: https://doi.org/10.1007/s00394-019-01910-z

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