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Effects of probiotics, prebiotics or synbiotics on jawbone in obese-insulin resistant rats

  • Sathima Eaimworawuthikul
  • Wannipa Tunapong
  • Titikorn Chunchai
  • Sakawdaurn Yasom
  • Keerati Wanchai
  • Panan Suntornsaratoon
  • Narattaphol Charoenphandhu
  • Parameth Thiennimitr
  • Nipon Chattipakorn
  • Siriporn C. Chattipakorn
Original Contribution

Abstract

Purpose

Chronic high-fat diet (HFD) consumption results in gut dysbiosis, systemic inflammation, obese-insulin resistance, and osteoporosis of the jawbones. The probiotics, prebiotics or synbiotics alleviated gut dysbiosis and the metabolic disturbance in HFD-induced obesity. However, the effects on jawbone properties have not been investigated. This study aimed to investigate the effects of probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on the jawbone properties along with metabolic parameters, gut and systemic inflammation in HFD-fed rats.

Methods

Forty-eight male Wistar rats were fed with either a HFD or normal diet for 12 weeks. Rats in each group were subdivided into four subgroups to be treated with either vehicle, probiotics, prebiotics, or synbiotics for the additional 12 weeks. Blood samples, gut, bone marrows, and jawbones were collected to determine metabolic parameters, inflammation, and bone properties.

Results

The HFD-fed rats developed obese-insulin resistance, as indicated by increased body weight, dyslipidemia and decreased insulin sensitivity. Serum lipopolysaccharide levels and interleukin-6 mRNA expression in the ileum and bone marrows were elevated. Altered bone metabolism and the impaired jawbone properties were evident as indicated by decreased bone mineral density with increased trabecular separation. Reduced ultimate load and stiffness were observed in HFD-fed rats. Treatments with probiotics, prebiotics or synbiotics in HFD-fed rats improved metabolic parameters and reduced inflammation. However, no alterations in jawbone properties were found in all treatments.

Conclusion

The osteoporosis of the jawbone occurred in obese-insulin resistance, and treatments with probiotics, prebiotics and synbiotics were not sufficient to improve the jawbone properties.

Keywords

Lactobacillus paracasei HII01 Xylooligosaccharide Synbiotics Obesity Insulin resistance Jawbone 

Notes

Acknowledgements

This work was supported by Thailand Research Fund (TRF) Grants: TRF-Senior Research Scholar RTA6080003 (to SCC), RTA6080007 (to N. Charoenphandhu), IRN60W0001 (to N. Charoenphandhu) and MRG 6180187 (to PT); a CMU 50th Anniversary grant by Chiang Mai University (PHD/014/2557 SE&SCC); a NSTDA Research Chair Grant from the National Science and Technology Development Agency Thailand (N. Chattipakorn) and a Chiang Mai University Center of Excellence Award (N. Chattipakorn).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sathima Eaimworawuthikul
    • 1
    • 2
    • 3
  • Wannipa Tunapong
    • 3
  • Titikorn Chunchai
    • 3
  • Sakawdaurn Yasom
    • 4
  • Keerati Wanchai
    • 3
  • Panan Suntornsaratoon
    • 5
    • 6
  • Narattaphol Charoenphandhu
    • 5
    • 6
    • 7
  • Parameth Thiennimitr
    • 4
  • Nipon Chattipakorn
    • 3
  • Siriporn C. Chattipakorn
    • 2
    • 3
    • 8
  1. 1.Department of Orthodontics and Pediatric Dentistry, Faculty of DentistryChiang Mai UniversityChiang MaiThailand
  2. 2.Department of Oral Biology and Diagnostic Sciences, Faculty of DentistryChiang Mai UniversityChiang MaiThailand
  3. 3.Center of Excellence in Cardiac Electrophysiology Research, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  4. 4.Department of Microbiology, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  5. 5.Center of Calcium and Bone Research (COCAB), Faculty of ScienceMahidol UniversityBangkokThailand
  6. 6.Department of Physiology, Faculty of ScienceMahidol UniversityBangkokThailand
  7. 7.Institute of Molecular BiosciencesMahidol UniversityNakhon PathomThailand
  8. 8.Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of MedicineChiang Mai UniversityChiang MaiThailand

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