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European Journal of Nutrition

, Volume 58, Issue 7, pp 2625–2638 | Cite as

Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice

  • Yueting Ge
  • Wei LiuEmail author
  • Haiteng Tao
  • Yu Zhang
  • Lina Liu
  • Zhenhua Liu
  • Bin QiuEmail author
  • Tongcheng Xu
Original Contribution

Abstract

Purpose

Previous studies have shown that industrially originated trans-fatty acids (iTFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of iTFA on gut microbiota, help understanding the underling mechanism of iTFA-associated chronic diseases.

Methods

Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non-iTFA) or partially hydrogenated soybean oil (iTFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography.

Results

The intake of iTFA increased the abundance of well-documented ‘harmful’ bacteria, such as Proteobacteria and Desulfovibrionaceae (P < 0.05), whereas it decreased relative abundance of ‘beneficial’ bacteria, such as Bacteroidetes, Lachnospiraceae, Bacteroidales S24-7 (P < 0.05). Surprisingly, the intake of iTFA increased the abundance of the probiotic Lactobacillaceae (P < 0.05). Additionally, the intake of iTFA induced increase of inflammatory parameters, as well as a numerical decrease of fecal butyric acid and valeric acid.

Conclusions

This study, to our knowledge, is the first to demonstrate that the consumption of iTFA resulted in a significant dysbiosis of gut microbiota, which may contribute to the development of chronic diseases associated with iTFA.

Keywords

Trans-fatty acid Gut microbiota Short-chain fatty acid 16S rRNA gene sequencing 

Abbreviations

TFA

Trans-fatty acid

iTFA

Industrial originated trans-fatty acids

SCFA

Short-chain fatty acid

GC

Gas chromatography

IBD

Inflammatory bowel disease

SRB

Sulfate-reducing bacteria

IL6

Interleukin 6

TNF-α

Tumor necrosis factor alpha

NAFLD

Nonalcoholic fatty liver disease

NASH

Nonalcoholic steatohepatitis

Notes

Funding

This project is supported by National Natural Science Foundation of China (31401487).

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

394_2018_1810_MOESM1_ESM.docx (425 kb)
Supplementary material 1 (DOCX 424 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources ProcessingMinistry of AgricultureJinanChina
  2. 2.The Laboratory of Food Nutrition and Functional Factors, School of Food Science and TechnologyJiangnan UniversityWuxiChina
  3. 3.State Key Laboratory of Biobased Material and Green Papermaking, College of Food Science and EngineeringQilu University of Technology (Shandong Academy of Sciences)JinanChina
  4. 4.School of Public Health and Health SciencesUniversity of MassachusettsAmherstUSA

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