Polyphenol-rich curry made with mixed spices and vegetables benefits glucose homeostasis in Chinese males (Polyspice Study): a dose–response randomized controlled crossover trial
- 132 Downloads
To investigate acute effects of two doses of a polyphenol-rich curry made with seven different spices and four base vegetables, eaten with white rice, on 24 h glucose response, postprandial insulinemia, triglyceridemia and 24 h urinary total polyphenol excretion (TPE).
Randomized, controlled, dose–response crossover trial in healthy, Chinese men [n = 20, mean ± standard deviation (SD) age 23.7 ± 2.30 years, BMI 23.0 ± 2.31 kg/m2] who consumed test meals matched for calories, macronutrients and total vegetables content, consisting either Dose 0 Control (D0C) or Dose 1 Curry (D1C) or Dose 2 Curry (D2C) meal. 24 h glucose concentration was measured using continuous glucose monitoring (CGM), together with postprandial plasma insulin and triglyceride for up to 7 h. Total polyphenol content (TPC) of test meals and urinary TPE were measured using the Folin–Ciocalteu assay.
TPC for D0C, D1C and D2C were 130 ± 18, 556 ± 19.7 and 1113 ± 211.6 mg gallic acid equivalent (GAE) per portion served, respectively (p < 0.0001). Compared with D0C meal, we found significant linear dose–response reductions in the 3-h postprandial incremental AUC (iAUC) for CGM glucose of 19% and 32% during D1C and D2C meals respectively (p < 0.05) and non-significant linear dose response reductions in iAUC of insulin (p = 0.089). Notably, we found significant dose-dependent increases in postprandial triglyceride with increasing curry doses (p < 0.01). Significant increases in TPE with increasing curry doses were also observed (p < 0.01).
Polyphenol-rich curry intake can improve postprandial glucose homeostasis. The longer term effects remain to be established.
KeywordsSpices Curry Polyphenols Continuous glucose monitoring Postprandial insulinemia Postprandial triglyceridemia
The authors thank Professor Zhou Weibiao, from the Food Science and Technology Program, c/o Department of Chemistry, National University of Singapore for the use the blast freezer in his laboratory for the preparation of the test meals for the study. They also thank Susanna Lim for her assistance with phlebotomy and the volunteers for taking part in this study. This project was funded by the Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*STAR), Singapore.
SH and CJH was involved in developing the concept of the study; SH, CJH, MKSL, ECYC, SCC designed the research; SCC, SH, SHL and JL conducted research; SCC, SH, SHL analyzed the data or performed statistical analysis; SH, SCC, CJH wrote the manuscript and all authors had read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- 2.Tresserra-Rimbau A, Guasch-Ferré M, Salas-Salvadó J, Toledo E, Corella D, Castañer O, Guo X, Gómez-Gracia E, Lapetra J, Arós F (2016) Intake of total polyphenols and some classes of polyphenols is inversely associated with diabetes in elderly people at high cardiovascular disease risk. J Nutr 146(4):767–777CrossRefGoogle Scholar
- 3.Hooper L, Kroon PA, Rimm EB, Cohn JS, Harvey I, Le Cornu KA, Ryder JJ, Hall WL, Cassidy A (2008) Flavonoids, flavonoid-rich foods, and cardiovascular risk: a meta-analysis of randomized controlled trials. Am J Clin Nutr 88(1):38–50Google Scholar
- 4.Tresserra-Rimbau A, Rimm EB, Medina-Remón A, Martínez-González M, De la Torre R, Corella D, Salas-Salvadó J, Gómez-Gracia E, Lapetra J, Arós F (2014) Inverse association between habitual polyphenol intake and incidence of cardiovascular events in the PREDIMED study. Nutr Metab Cardiovasc Dis 24(6):639–647CrossRefGoogle Scholar
- 8.Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79(5):727–747Google Scholar
- 16.Liu RH (2003) Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78(3):517S-520SGoogle Scholar
- 18.Skulas-Ray AC, Kris-Etherton PM, Teeter DL, Chen CO, Heuvel JPV, West SG (2011) A high antioxidant spice blend attenuates postprandial insulin and triglyceride responses and increases some plasma measures of antioxidant activity in healthy, overweight men. J Nutr 141(8):1451–1457CrossRefGoogle Scholar
- 21.Tantipopipat S, Boonpraderm A, Charoenkiatkul S, Wasantwisut E, Winichagoon P (2010) Dietary intake of spices and herbs in habitual Northeast Thai diets. Mal J Nutr 16(1):137–148Google Scholar
- 23.Medina-Remón A, Barrionuevo-González A, Zamora-Ros R, Andres-Lacueva C, Estruch R, Martínez-González M-Á, Diez-Espino J, Lamuela-Raventos RM (2009) Rapid Folin–Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake. Anal Chim Acta 634(1):54–60CrossRefGoogle Scholar
- 29.Lampe JW (2009) Interindividual differences in response to plant-based diets: implications for cancer risk. Am J Clin Nutr 89(5):1553S-1557SGoogle Scholar
- 30.Manach C, Milenkovic D, de Wiele T, Rodriguez-Mateos A, Roos B, Garcia-Conesa MT, Landberg R, Gibney ER, Heinonen M, Tomás-Barberán F (2017) Addressing the inter-individual variation in response to consumption of plant food bioactives—towards a better understanding of their role in healthy ageing and cardiometabolic risk reduction. Mol Nutr Food Res 61(6):1600557CrossRefGoogle Scholar
- 31.Scalbert A, Williamson G (2000) Dietary intake and bioavailability of polyphenols. J Nutr 130(8):2073S–2085SGoogle Scholar
- 41.Milenkovic D, Morand C, Cassidy A, Konic-Ristic A, Tomás-Barberán F, Ordovas JM, Kroon P, De Caterina R, Rodriguez-Mateos A (2017) Interindividual variability in biomarkers of cardiometabolic health after consumption of major plant-food bioactive compounds and the determinants involved. Adv Nutr 8(4):558–570Google Scholar
- 42.González-Sarrías A, Combet E, Pinto P, Mena P, Dall’Asta M, Garcia-Aloy M, Rodríguez-Mateos A, Gibney ER, Dumont J, Massaro M (2017) A systematic review and meta-analysis of the effects of flavanol-containing tea, cocoa and apple products on body composition and blood lipids: exploring the factors responsible for variability in their efficacy. Nutrients 9(7):746CrossRefGoogle Scholar
- 44.Hlebowicz J, Hlebowicz A, Lindstedt S, Björgell O, Höglund P, Holst JJ, Darwiche G, Almer L-O (2009) Effects of 1 and 3 g cinnamon on gastric emptying, satiety, and postprandial blood glucose, insulin, glucose-dependent insulinotropic polypeptide, glucagon-like peptide 1, and ghrelin concentrations in healthy subjects. Am J Clin Nutr 89(3):815–821CrossRefGoogle Scholar
- 52.Hlebowicz J, Darwiche G, Björgell O, Almér L-O (2007) Effect of cinnamon on postprandial blood glucose, gastric emptying, and satiety in healthy subjects. Am J Clin Nutr 85(6):1552–1556Google Scholar
- 55.Ahuja KD, Robertson IK, Geraghty DP, Ball MJ (2006) Effects of chili consumption on postprandial glucose, insulin, and energy metabolism. Am J Clin Nutr 84(1):63–69Google Scholar
- 58.Mozaffari-Khosravi H, Talaei B, Jalali B-A, Najarzadeh A, Mozayan MR (2014) The effect of ginger powder supplementation on insulin resistance and glycemic indices in patients with type 2 diabetes: a randomized, double-blind, placebo-controlled trial. Complement Ther Med 22(1):9–16CrossRefGoogle Scholar
- 60.McCrea CE, West SG, Kris-Etherton PM, Lambert JD, Gaugler TL, Teeter DL, Sauder KA, Gu Y, Glisan SL, Skulas-Ray AC (2015) Effects of culinary spices and psychological stress on postprandial lipemia and lipase activity: results of a randomized crossover study and in vitro experiments. J Transl Med 13(1):7CrossRefGoogle Scholar
- 61.Botelho FV, Enéas LR, Cesar GC, Bizzotto CS, Tavares É, Oliveira FcA, Gloria MBA, Silvestre MP, Arantes RM, Alvarez-Leite JI (2004) Effects of eggplant (Solanum melongena) on the atherogenesis and oxidative stress in LDL receptor knock out mice (LDLR–/–). Food Chem Toxicol 42(8):1259–1267CrossRefGoogle Scholar
- 64.Jenkins DJ, Kendall CW, Marchie A, Faulkner DA, Wong JM, de Souza R, Emam A, Parker TL, Vidgen E, Trautwein EA (2005) Direct comparison of a dietary portfolio of cholesterol-lowering foods with a statin in hypercholesterolemic participants. Am J Clin Nutr 81(2):380–387Google Scholar