European Journal of Nutrition

, Volume 58, Issue 1, pp 291–300 | Cite as

The influence of isoflavone for denervation-induced muscle atrophy

  • Shinpei Tabata
  • Miki Aizawa
  • Masakazu Kinoshita
  • Yoshinori Ito
  • Yusuke Kawamura
  • Minoru Takebe
  • Weijun Pan
  • Kunihiro SakumaEmail author
Original Contribution



Decrease in activity stress induces skeletal muscle atrophy. A previous study showed that treatment with a high level (20%) of isoflavone inhibits muscle atrophy after short-term denervation (at 4 days) in mice. The present study was designed to elucidate whether the dietary isoflavone aglycone (AglyMax) at a 0.6% prevents denervation-mediated muscle atrophy, based on the modulation of atrogin-1- or apoptosis-dependent signaling.


Mice were fed either a normal diet or 0.6% AglyMax diet. One week later, the right sciatic nerve was cut. The wet weight, mean fiber area, amount of atrogin-1 and cleaved caspase-3 proteins, and the percentages of apoptotic nuclei were examined in the gastrocnemius muscle at 14 days after denervation.


The 0.6% AglyMax diet significantly attenuated denervation-induced decreases in fiber atrophy but not the muscle wet weight. In addition, dietary isoflavone suppressed the denervation-induced apoptosis in spite of there being no significant changes in the amount of cleaved caspase-3 protein. In contrast, the 0.6% AglyMax diet did not significantly modulate the protein expression of atrogin-1 in the denervated muscle of mice.


The isoflavone aglycone (AglyMax) at a 0.6% significantly would modulate muscle atrophy after denervation in mice, probably due to the decrease in apoptosis-dependent signaling.


Isoflavone Muscle atrophy Supplementation Atrogin-1 Apoptosis Denervation 



This work was supported by a research Grant-in-Aid for Scientific Research C (No. 17K01755) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Compliance with ethical standards

Conflict of interest

Kunihiro Sakuma and all the co-authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Research Center for Physical Fitness, Sports and HealthToyohashi University of TechnologyToyohashiJapan
  2. 2.Institute for Liberal Arts, Environment and SocietyTokyo Institute of TechnologyTokyoJapan
  3. 3.Nichimo Biotics CompanyTokyoJapan

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