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Oxidative damage of mitochondrial respiratory chain in different organs of a rat model of diet-induced obesity

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Abstract

Purpose

Mitochondrial dysfunction plays an important role in the development of obesity and obesity-associated metabolic diseases.

Methods

In this study, we dynamically observed the characteristics of mitochondrial damage in a rat model of diet-induced obesity (DIO). From the 2nd to the 10th week, animals were killed every 2 weeks and the heart, liver, kidney, and testicular tissues were harvested. Mitochondria were isolated and the activities of respiratory chain complexes I, II, III, and IV as well as the 8-Hydroxy-2-deoxy Guanosine content were determined. Reactive oxygen species and malondialdehyde were measured.

Results

Mitochondrial damages were observed in the heart and liver of DIO and DR rats, and the damages occurred later in DR group than that in DIO group. The mitochondrial membrane potential of heart and liver decreased in DIO and DR groups. The activity of the heart mitochondria complexes I, III, and IV (composing NADH oxidative respiratory) was higher in the early stage of DIO and lower in the end of week 10. The higher activity of the liver complexes I, III, and IV was found until the end of week 10 in DIO and DR groups, accompanied with enhanced oxidative stress. Besides, mitochondrial DNA damages were observed in all tissues.

Conclusion

In DIO rats, the heart mitochondrial dysfunction occurred first and the liver presented the strongest compensatory ability against oxidative stress.

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Acknowledgements

This work was supported by research Grants from the National Natural Science Foundation of China (81072297 and 81472964). Chang Bai Shan Scholars Program of Jilin Province, China.

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Author notes

  1. Hai-Tao Yu and Xiao-Yi Fu contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Jilin Medical University, 5 Jilin Street, FengMan District, Jilin City, 132013, Jilin, China

    Hai-Tao Yu, Xiao-Yi Fu & Shu-Ran Wang

  2. Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi’an Jiaotong University, 28 W. Xian-ning Road, Xi’an, 710049, Shanxi, China

    Jian-Kang Liu & Zhi-Hui Feng

  3. HaiNan Branch of P.L.A. General Hospital, Sanya, 572014, Hainan, China

    Bing Liang

  4. Center for Disease Control and Prevention, TongZhou, Beijing, 101100, China

    Shuang Wang

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  1. Hai-Tao Yu
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  2. Xiao-Yi Fu
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Correspondence to Shu-Ran Wang or Zhi-Hui Feng.

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We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work; there is no conflict of interest.

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Yu, HT., Fu, XY., Liang, B. et al. Oxidative damage of mitochondrial respiratory chain in different organs of a rat model of diet-induced obesity. Eur J Nutr 57, 1957–1967 (2018). https://doi.org/10.1007/s00394-017-1477-0

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  • DOI: https://doi.org/10.1007/s00394-017-1477-0

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