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European Journal of Nutrition

, Volume 57, Issue 2, pp 751–760 | Cite as

Determinants of folate and vitamin B12 plasma levels in the French E3N-EPIC cohort

  • Jordi de BatlleEmail author
  • Marco Matejcic
  • Veronique Chajes
  • Hortensia Moreno-Macias
  • Amina Amadou
  • Nadia Slimani
  • David G. Cox
  • Françoise Clavel-Chapelon
  • Guy Fagherazzi
  • Isabelle Romieu
Original Contribution

Abstract

Purpose

Impaired B vitamin status has been identified as a risk factor for major chronic diseases. This study aims at examining the determinants of plasma folate and vitamin B12 concentrations, considering lifestyle factors and MTHFR polymorphisms.

Methods

A total of 988 women aged 40–65 years from the French E3N cohort were investigated. Intakes of folate and vitamin B12 were assessed using food frequency questionnaires, and plasma concentrations were measured by microbiological assay. Dietary scores were computed to summarize folate and vitamin B12 dietary sources. MTHFR-C677T and MTHFR-A1298C were determined by Kaspar assay. Pearson’s partial correlation coefficients and multivariable linear regression models were used to assess correlations between main determinants and plasma folate and vitamin B12 levels.

Results

The partial correlation coefficient between dietary intakes and plasma folate was 0.19 (p value <0.001) and 0.08 (p value = 0.008) for vitamin B12. Dietary scores were the main determinant of B vitamin plasma concentrations with a percent change per unit increase of 12.64% (p value <0.001) for folate and 7.6% (p value <0.001) for vitamin B12. Homozygous (T/T) or heterozygous (C/T) women for MTHFR-C677T had lower plasma folate concentrations [C/T: −6.48% (p value = 0.038) and T/T: −15.89% (p value <0.001)] compared to women carrying the C/C genotype. Other determinants of B vitamin plasma concentration include: smoking status for folate, and age and hormone replacement therapy for vitamin B12.

Conclusions

We confirmed previous findings on the role of diet as main determinant of folate and vitamin B12 plasma concentrations. However, the impact of genetic polymorphisms and lifestyle factors on plasma B vitamin concentrations should not be neglected.

Keywords

Folate Vitamin B12 Dietary intake Biomarkers MTHFR polymorphisms 

Notes

Acknowledgements

This work was supported by Fondation de France, World Cancer Research Funds (WCRF), Institut National du Cancer (INCA), and La Ligue Nationale contre le Cancer. Jordi de Batlle acknowledges the support of the European Commission FP7 Marie Curie Actions-People-Cofunding of regional, national and international programs (COFUND). The authors thank Deborah Postoly, laboratory technician, for sample processing and genotyping of study samples.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

All participants signed an informed consent prior to their inclusion in the study. The study has been approved by the ethics committees of all participating centers and has therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

Supplementary material

394_2016_1365_MOESM1_ESM.docx (436 kb)
Supplementary material 1 (DOCX 435 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jordi de Batlle
    • 1
    Email author
  • Marco Matejcic
    • 1
  • Veronique Chajes
    • 1
  • Hortensia Moreno-Macias
    • 2
  • Amina Amadou
    • 1
  • Nadia Slimani
    • 1
  • David G. Cox
    • 3
  • Françoise Clavel-Chapelon
    • 4
    • 5
    • 6
  • Guy Fagherazzi
    • 4
    • 5
    • 6
  • Isabelle Romieu
    • 1
  1. 1.International Agency for Research on Cancer (IARC)LyonFrance
  2. 2.Universidad Autónoma MetropolitanaMexico CityMexico
  3. 3.INSERM U1052Cancer Research Center of Lyon, Centre Léon BérardLyonFrance
  4. 4.Team 9: Lifestyle, Genes and Health: Integrative Trans-generational Epidemiology, Center for Research in Epidemiology and Population HealthInstitut National de la Santé et de la Recherche Médicale (INSERM), U1018VillejuifFrance
  5. 5.Université Paris-SudVillejuifFrance
  6. 6.Gustave Roussy InstituteVillejuifFrance

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