Iron bioavailability from supplemented formula milk: effect of lactoferrin addition
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In this work, the absorption and/or bioavailability of iron from two chemical species, 57Fe-Lf (apo-lactoferrin) complex and 57FeSO4 at low and high dose, and in Lf excess were investigated in lactating wistar rats.
The methodology used is based on the use of stable isotopes in combination with the approach “isotope pattern deconvolution” and ICP-MS for detection. This approach provides quantitative information about exogenous (57Fe) and endogenous iron (natFe) distribution in fluids and tissues in the iron-supplemented rat groups.
The observed results with supplemented rats were compared with those found in rats receiving maternal feeding. Interestingly, differences were found between groups in iron for transport and storage compartments, but not in the functional one, depending upon the dose of iron administered and the chemical species.
Considering the results obtained, supplementation with iron salts in excess of Lf appears to be the best way of iron supplementation of formula milk.
KeywordsIPD ICP-MS Lf Iron supplementation Enriched stable isotopes Lactating rats Apparent absorption retention Endogenous exogenous iron Body tissues fluids
M. L. F. S. and A. S. M. designed research; S. F. M. conducted research; B. F. C., J. L. S, H. G. I. and S. F. M. analysed data; S. F. M., M. L. F. S. and A. S. M. wrote the paper. All authors have read and approved the final manuscript. On behalf of all authors, the corresponding author states that there is no conflict of interest. Authors are grateful to “Fundación para la Investigación Científica Aplicada y la Tecnología, Principado de Asturias” (FICYT. FC-11-PC10-46) and to “Fondo Europeo de Desarrollo Regional” (FEDER). Also financial support from “Laboratorios Ordesa” (Barcelona, Spain) and “Fundación Grupo Castrillo” (Spain) is gratefully acknowledged.
- 2.Gisbert JP, Gomollón F (2009) An update on iron physiology. World J Gastroenterol 15:4617–4626Google Scholar
- 3.Mertz W, Underwood EJ (1986) Trace elements in human and animal nutrition. Academic Press Inc., Nueva York (EEUU). ISBN 0-1249-1252-4Google Scholar
- 6.Fernández-Sánchez ML, de la Flor St. Remy R, González-Iglesias H, López-Sastre JB, Fernández-Colomer B, Pérez-Solís D, Sanz-Medel A (2012) Iron content and its speciation in human milk from mothers of preterm and full-term infants at early stages of lactation: a comparison with commercial infant milk formulas. Microchem J 105:108–114. doi: 10.1016/j.microc.2012.03.016 Google Scholar
- 10.García-Alonso JI, Rodriguez-González P (2013) Isotope dilution mass spectrometry. The Royal Society of Chemistry Publishing, London. ISBN 978-1-84973-333-5Google Scholar
- 13.Messerschmidt A, Huber R, Poulos T, Wieghardt K (2011) Handbook of metalloproteins, vol 2. Wiley, New York. ISBN 9780000000298Google Scholar
- 15.Codex Stand, Standard for Infant Formula and Formulas for Special Medical Purposes Intended for Infants (1981; Amendment 2011) Ref. 72-1981. Food and Agriculture Organization of the United Nations, World Health Organization. http://www.codexalimentarius.org/standards/list-of-standards/en/?provide=standards&orderField=fullReference&sort=asc&num1=CODEX (visited on 14th December 2015)
- 16.Frazer DM, Wilkins SJ, Anderson GJ (2007) Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract. Am J Physiol Gastrointest Liver Physiol 293:525–531Google Scholar
- 20.Gürsel FE, Ates A, Bilal T, Altiner A (2012) Effect of dietary garcinia cambogia extract on serum essential minerals (calcium, phosphorus, magnesium and trace elements (iron, copper, zinc) in rats fed with high-lipid diet. Biol Trace Elem Res 148:378–382. doi: 10.1007/s12011-012-9385-x PubMedGoogle Scholar