Probiotics modulate gut microbiota and health status in Japanese cedar pollinosis patients during the pollen season
- 933 Downloads
Japanese cedar pollinosis (JCP) is a challenging public health problem in Japan. Altered gut microbiota is associated with several diseases, including allergic diseases. However, only a few studies have focused on JCP and the underlying mechanisms for probiotic effects remain unclear. In addition, this study is the first observation of the correlation between the gut microbiota and blood lipid in JCP.
Faecal samples from JCP subjects were collected before and after treatment with (n = 14) and without (n = 11) LGG–TMC0356-fermented milk for 10 weeks. Gut microbiota composition was characterized from faecal DNA using sequencing of 16S rRNA genes.
16S rRNA-based operational taxonomic unit clustering of the microbiota revealed that LGG–TMC0356-fermented milk significantly altered gut microbiota after 10 weeks of milk consumption, and eight dominant genera of microbes were detected. During the JCP season, the Bacteroidetes/Firmicutes ratio, when compared to baseline, was significantly decreased in subjects at end of the study. Bacteroidetes showed positive correlation with LDL- and HDL-cholesterol levels, whereas Firmicutes showed negative correlation with total cholesterol, LDL- and HDL- cholesterol.
The altered gut microbiota through supplementation of fermented milk containing the study probiotics may be a prospective target for protection against JCP, with beneficial effects on blood lipid levels.
KeywordsJCP Gut microbiota Blood lipid Lactobacillus rhamnosus GG Lactobacillus gasseri TMC0356 Probiotic Allergy
This work supported by Takanashi milk Company Limited. We thank Dr. Juha-Pekka Pursiheimo (Turku Clinical Sequencing Laboratory, Institute of Biomedicine, University of Turku) for his assistance and technique of next-generation sequencing. Thanks also to Enago (www.enago.jp) for the English language review.
This work was supported by Takanashi Milk Products Co., Ltd.
Compliance with ethical standards
Conflict of interest
G. Harata, F. He, K. Miyazawa, K. Yoda, M. Kawase, A. Kubota and M. Hiramatsu are employees of Takanashi Milk Products Co., Ltd.; H. Kumar, S. Rautava and S. Salminen declare no conflicts of interest.
- 1.Fiocchi A, Pawankar R, Cuello-Garcia C, Ahn K, Al-Hammadi S, Agarwal A, Beyer K, Burks W, Canonica GW, Ebisawa M et al (2015) World Allergy Organization-McMaster University Guidelines for Allergic Disease Prevention (GLAD-P): Probiotics. World Allergy Organ J 8:4. doi: 10.1186/s40413-016-0102-7 CrossRefGoogle Scholar
- 2.Okuda M, Ohkubo K, Gotoh M, Hiroshima K, Ishida Y, Hori K (2005) Dynamics of airborne pollen particles from inhalation to allergic reaction in the nose. Rhinology 43:29–33Google Scholar
- 6.Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S et al (2014) Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11:506–514. doi: 10.1038/nrgastro.2014.66 CrossRefGoogle Scholar
- 8.Kawase M, He F, Kubota A, Hiramatsu M, Saito H, Ishii T, Yasueda H, Akiyama KE (2009) Effect of fermented milk prepared with two probiotic strains on Japanese cedar pollinosis in a double-blind placebo-controlled clinical study. Int J Food Microbiol 128:429–434. doi: 10.1016/j.ijfoodmicro.2008.09.017 CrossRefGoogle Scholar
- 16.Tamura M, Shikina T, Morihana T, Hayama M, Kajimoto O, Sakamoto A, Kajimoto Y, Watanabe O, Nonaka C, Shida K et al (2007) Effects of probiotics on allergic rhinitis induced by Japanese cedar pollen: randomized double-blind, placebo-controlled clinical trial. Int Arch Allergy Immunol 143:75–82CrossRefGoogle Scholar
- 21.Nylund L, Satokari R, Nikkilä J, Rajilić-Stojanović M, Kalliomäki M, Isolauri E, Salminen S, de Vos WM (2013) Microarray analysis reveals marked intestinal microbiota aberrancy in infants having eczema compared to healthy children in at-risk for atopic disease. BMC Microbiol 23(13):12. doi: 10.1186/1471-2180-13-12 CrossRefGoogle Scholar
- 23.Burger-van Paassen N, Vincent A, Puiman PJ, van der Sluis M, Bouma J, Boehm G, van Goudoever JB, van Seuningen I, Renes IB (2009) The regulation of intestinal mucin MUC2 expression by short-chain fatty acids: implications for epithelial protection. Biochem J 420:211–219. doi: 10.1042/BJ20082222 CrossRefGoogle Scholar
- 30.Berni Canani R, Sangwan N, Stefka AT, Nocerino R, Paparo L, Aitoro R, Calignano A, Khan AA, Gilbert JA, Nagler CR (2016) Lactobacillus rhamnosus GG-supplemented formula expands butyrate-producing bacterial strains in food allergic infants. ISME J 10:742–750. doi: 10.1038/ismej.2015.151 CrossRefGoogle Scholar
- 31.Feleszko W, Jaworska J, Rha RD, Steinhausen S, Avagyan A, Jaudszus A, Ahrens B, Groneberg DA, Wahn U, Hamelmann E (2007) Probiotic-induced suppression of allergic sensitization and airway inflammation is associated with an increase of T regulatory-dependent mechanisms in a murine model of asthma. Clin Exp Allergy 37:498–505CrossRefGoogle Scholar
- 35.Maksimoval OV, Zaitseva EV, Mazurina SA, Revyakina VA, Gervazieva VB (2015) Intestine microbiota in children with obesity and allergic diseases. Zh Mikrobiol Epidemiol Immunobiol 3(53–8):32Google Scholar