European Journal of Nutrition

, Volume 56, Issue 6, pp 2181–2191 | Cite as

Chronic pistachio intake modulates circulating microRNAs related to glucose metabolism and insulin resistance in prediabetic subjects

Original Contribution

Abstract

Purpose

To assess the effects of a pistachio-enriched diet on the profile of circulating microRNAs (miRNAs) related to glucose metabolism and insulin resistance (IR).

Methods

Randomized crossover clinical trial in 49 subjects with prediabetes was performed. Subjects consumed a pistachio-supplemented diet (PD, 50 % carbohydrates, 33 % fat, including 57 g/day of pistachios) and an isocaloric control diet (CD, 55 % carbohydrates and 30 % fat) for 4 months each, separated by a 2-week washout period. The plasma profile of a set of seven predefined miRNAs related to glucose and insulin metabolism was analyzed by quantitative RT-PCR.

Results

After the PD period, subjects have shown significant lower circulating levels of miR-192 and miR-375 compared to CD period, whereas miR-21 nonsignificantly increased after PD compared with CD (47 vs. 2 %, P = 0.092). Interestingly, changes in circulating miR-192 and miR-375 were positively correlated with plasma glucose, insulin and HOMA-IR.

Conclusion

Chronic pistachio consumption positively modulates the expression of some miRNA previously implicated on insulin sensitivity.

Keywords

Prediabetes Pistachio microRNA Glucose 

Supplementary material

394_2016_1262_MOESM1_ESM.docx (52 kb)
Supplementary material 1 (DOCX 51 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Pablo Hernández-Alonso
    • 1
    • 2
  • Simona Giardina
    • 1
  • Jordi Salas-Salvadó
    • 1
    • 2
  • Pierre Arcelin
    • 3
  • Mònica Bulló
    • 1
    • 2
  1. 1.Human Nutrition Unit, Biochemistry and Biotechnology Department, Faculty of Medicine and Health Sciences, University Hospital of Sant Joan de Reus, IISPVUniversitat Rovira i VirgiliReusSpain
  2. 2.CIBERobn Physiopathology of Obesity and NutritionInstituto de Salud Carlos IIIMadridSpain
  3. 3.ABS Reus V. Centre d’Assistència Primària Marià FortunySAGESSAReusSpain

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