Wine consumption reduced postprandial platelet sensitivity against platelet activating factor in healthy men
Platelet activating factor (PAF) is a potent inflammatory and thrombotic mediator that participates in the initiation and prolongation of atherosclerosis. The aim of the present study was to evaluate the potential effect of wine consumption on platelet aggregation against PAF.
The study had cross-over design. Ten healthy men participated in four daily trials on separate days: They consumed a standardized meal along with white wine, Robola variety (trial R), or red wine, Cabernet Sauvignon variety (trial CS), or an ethanol solution (trial E), or water (trial W). Blood samples were collected before and after meal consumption and at several time points during the next 6 h. Platelet aggregation against PAF (EC50 values) and several blood biomarkers were measured, and incremental areas under the curve (iAUC) were calculated.
A significant trial effect was found in platelet sensitivity against PAF (p trial = 0.01). Moreover, the iAUC–PAF EC50 of CS trial was higher compared to both iAUC–PAF EC50 of E and W trials (P = 0.04, P = 0.02). Plasminogen activator inhibitor-1 iAUC was higher in all alcoholic beverages compare with the one of W trial (P E = 0.05, P R = 0.01, P CS = 0.01). Triacylglycerol iAUC increased significantly only in E compared to W trial (P = 0.04) and were significantly lower at 60–120 min in wine trials compared to the one of E (P < 0.05).
Wine consumption improved platelet sensitivity independently of alcohol, kept triacylglycerols at lower levels during their postprandial elevation, and did not affect plasminogen activator inhibitor-1 levels more adversely than ethanol per se.
KeywordsThrombosis Inflammation Microconstituents Phenolic compounds Alcohol Postprandial
We would like to thank Antigoni Tsiafitsa for her technical assistance in blood samples collection. The wines used in the study were kindly offered by Domaine Hatzimichalis.
The study was supported through a research funding from the Graduate Program of the Department of Nutrition and Dietetics, Harokopio University.
Compliance with ethical standards
Conflict of interest
The authors declare no competing financial interest.
- 9.Calabriso N, Scoditti E, Massaro M, Pellegrino M, Storelli C, Ingrosso I, Giovinazzo G, Carluccio MA (2015) Multiple anti-inflammatory and anti-atherosclerotic properties of red wine polyphenolic extracts: differential role of hydroxycinnamic acids, flavonols and stilbenes on endothelial inflammatory gene expression. Eur J Nutr. doi: 10.1007/s00394-015-0865-6 Google Scholar
- 19.Karatzi K, Papamichael C, Aznaouridis K, Karatzis E, Lekakis J, Matsouka C, Boskou G, Chiou A, Sitara M, Feliou G, Kontoyiannis D, Zampelas A, Mavrikakis M (2004) Constituents of red wine other than alcohol improve endothelial function in patients with coronary artery disease. Coron Artery Dis 15(8):485–490. doi: 10.1097/00019501-200412000-00005 CrossRefGoogle Scholar
- 20.Blanco-Colio LM, Valderrama M, Alvarez-Sala LA, Bustos C, Ortego M, Hernández-Presa MA, Cancelas P, Gómez-Gerique J, Millán J, Egido J (2000) Red wine intake prevents nuclear factor-kappaB activation in peripheral blood mononuclear cells of healthy volunteers during postprandial lipemia. Circulation 102(9):1020–1026. doi: 10.1161/01.cir.102.9.1020 CrossRefGoogle Scholar
- 21.Tousoulis D, Ntarladimas I, Antoniades C, Vasiliadou C, Tentolouris C, Papageorgiou N, Latsios G, Stefanadis C (2008) Acute effects of different alcoholic beverages on vascular endothelium, inflammatory markers and thrombosis fibrinolysis system. Clin Nutr 27(4):594–600. doi: 10.1016/j.clnu.2008.01.002 CrossRefGoogle Scholar
- 22.Numminen H, Kobayashi M, Uchiyama S, Iwata M, Ikeda Y, Riutta A, Syrjala M, Kekomaki R, Hillbom M (2000) Effects of alcohol and the evening meal on shear-induced platelet aggregation and urinary excretion of prostanoids. Alcohol Alcohol 35:594–600. doi: 10.1093/alcalc/35.6.594 CrossRefGoogle Scholar
- 23.Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am J Enol Vitic 16:144–158Google Scholar
- 25.Antonopoulou S, Fragopoulou E, Karantonis HC, Mitsou E, Sitara M, Rementzis J, Mourelatos A, Ginis A, Phenekos C (2006) Effect of traditional Greek Mediterranean meals on platelet aggregation in normal subjects and in patients with type 2 diabetes mellitus. J Med Food 9(3):356–362. doi: 10.1089/jmf.2006.9.356 CrossRefGoogle Scholar
- 29.Smith A, Patterson C, Yarnell J, Rumley A, Ben-Shlomo Y, Lowe G (2005) Which hemostatic markers add to the predictive value of conventional risk factors for coronary heart disease and ischemic stroke? The Caerphilly Study. Circulation 112:3080–3087. doi: 10.1161/circulationaha.105.557132 CrossRefGoogle Scholar
- 30.Byrne CD, Wareham NJ, Martensz ND, Humphries SE, Metcalfe JC, Grainger DJ (1998) Increased PAI activity and PAI-1 antigen occurring with an oral fat load: associations with PAI-1 genotype and plasma active TGF-beta levels. Atherosclerosis 140:45–53. doi: 10.1016/s0021-9150(98)00108-7 CrossRefGoogle Scholar
- 32.Siler SQ, Neese RA, Hellerstein MK (1999) De novo lipogenesis, lipid kinetics and whole-body lipid balances in humans after acute alcohol consumption. Am J Clin Nutr 70(5):928–936Google Scholar