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European Journal of Nutrition

, Volume 56, Issue 3, pp 1077–1084 | Cite as

Effect of oleic acid on store-operated calcium entry in immune-competent cells

  • Celia CarrilloEmail author
  • María Giraldo
  • M. Mar Cavia
  • Sara R. Alonso-Torre
Original Contribution

Abstract

Purpose

To study the mechanism by which oleic acid (OA) (C18:1) exerts its beneficial effects on immune-competent cells. Since store-operated Ca2+ entry (SOCE) is a Ca2+ influx pathway involved in the control of multiple physiological processes including cell proliferation, we studied the effect of OA in Ca2+ signals of Jurkat T cells and THP-1 monocytes, paying particular attention to SOCE.

Methods

Changes in [Ca2+]i were measured using the Fura-2 fluorescence dye. Mn2+ uptake was monitored as a rate of quenching of Fura-2 fluorescence measured at the Ca2+-insensitive wavelengths. Thapsigargin was used to induce SOCE in Fura-2-loaded cells.

Results

We showed a clear dose-dependent SOCE-inhibitory effect of OA in both cell lines. Such an inhibitory effect was PKC independent and totally restored by albumin, suggesting that OA exerts its effect somewhere in the membrane. We also demonstrated that OA induces increases in [Ca2+]i partly mediated by an extracellular Ca2+ influx through econazole-insensitive channels. Finally, we compared the effect of OA with stearic acid (C18:0), assuming the emerged evidence concerning the link between saturated fats and inflammation disorders. Stearic acid failed to inhibit SOCE, independently on the concentration tested, thus intensifying the physiological relevance of our findings.

Conclusion

We suggest a physiological pathway for the beneficial effects of OA in inflammation.

Keywords

Oleic acid Ca2+ signals SOCE Immune-competent cells 

Notes

Acknowledgments

We thank Gonzalo Moreno for his technical support and M.L. Nieto for providing us the cells.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Celia Carrillo
    • 1
    Email author
  • María Giraldo
    • 1
  • M. Mar Cavia
    • 1
  • Sara R. Alonso-Torre
    • 1
  1. 1.Nutrition and Food Science, Faculty of SciencesUniversity of BurgosBurgosSpain

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