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European Journal of Nutrition

, Volume 56, Issue 3, pp 1369–1373 | Cite as

(−)-Epicatechin attenuates high-glucose-induced inflammation by epigenetic modulation in human monocytes

  • Isabel Cordero-Herrera
  • Xinpu Chen
  • Sonia Ramos
  • Sridevi DevarajEmail author
Short Communication

Abstract

Purpose

Diabetes is a pro-inflammatory state associated with increased monocyte activity. NF-κB is the master switch of inflammation and is activated during diabetes. (−)-Epicatechin (EC), the main cocoa flavonol, displays anti-inflammatory and anti-diabetic effects under high glucose conditions. Recently, it has been suggested that dietary polyphenols might modulate chromatin remodelling by epigenetic changes and regulate monocyte NF-κB activation and cytokine expression under diabetic conditions. The aim of the study was to test the potential anti-inflammatory role of EC via inducing posttranslational histone changes in the presence of a high glucose (HG) concentrations.

Methods

Human monocytic cells (THP-1 cells) were pre-treated with EC (5 μM) and 4 h later exposed to 25 mM glucose (HG) for a total of 24 h. Control cells were grown under normoglycemic conditions (NG, 5.5 mM glucose). Acetyl CBP/p300, HDAC4, total histone 3 (HH3), H3K9ac, H3K4me2 and H3K9me2, and phosphorylated and total levels of p65-NF-κB were analysed by Western blot. Histone acetyltransferase (HAT) activity was measured in nuclear lysates, and TNF-α release was evaluated in culture media.

Results

EC incubation restored to control levels (NG) the changes induced by HG in p-p65/p65-NF-ĸB ratio, acetyl CBP/p300 values and HAT activity. Moreover, EC pre-treatment counteracted the increased acetylation of H3K9 and H3K4 dimethylation and attenuated the diminished H3K9 dimethylation triggered by HG. EC also significantly decreased HG-enhanced HDAC4 levels and TNF-α release, respectively.

Conclusions

EC induces epigenetic changes and decreased NF-κB and TNF-α levels in human monocytes cultured in HG conditions such as in diabetes.

Keywords

Diabetes Epicatechin Epigenetics Inflammation Human monocytes THP-1 cells Posttranslational histone modification 

Notes

Acknowledgments

I. Cordero-Herrera is a fellow of the FPI predoctoral program of MICINN.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Isabel Cordero-Herrera
    • 1
  • Xinpu Chen
    • 2
  • Sonia Ramos
    • 1
  • Sridevi Devaraj
    • 2
    Email author
  1. 1.Department of Metabolism and Nutrition, Institute of Food Science and Technology and Nutrition (ICTAN)Consejo Superior de Investigaciones Científicas (CSIC)MadridSpain
  2. 2.Department of Pathology & ImmunologyBaylor College of Medicine and Texas Children’s HospitalHoustonUSA

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