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European Journal of Nutrition

, Volume 55, Issue 3, pp 1261–1274 | Cite as

Green tea polyphenol extract in vivo attenuates inflammatory features of neutrophils from obese rats

  • K. F. F. S. Albuquerque
  • M. P. Marinovic
  • A. C. Morandi
  • A. P. Bolin
  • R. OttonEmail author
Original Contribution

Abstract

Purpose

Our study aimed to evaluate whether obesity induced by cafeteria diet changes the neutrophil effector/inflammatory function and whether treatment with green tea extract (GT) can improve neutrophil function.

Methods

Male Wistar rats were treated with GT by gavage (12 weeks/5 days/week; 500 mg/kg of body weight), and obesity was induced by cafeteria diet (8 weeks). Neutrophils were obtained from the peritoneal cavity (injection of oyster glycogen). The following analyses were performed: phagocytic capacity, chemotaxis, myeloperoxidase activity (MPO), hypochlorous acid (HOCl), superoxide anion (O 2 ·− ), hydrogen peroxide (H2O2), IL-1β, IL-6 and TNFα, mRNA levels of inflammatory genes, calcium mobilisation, activities of antioxidant enzymes, hexokinase and G6PDH.

Results

Neutrophils from obese rats showed a significant decrease in migration capacity, H2O2 and HOCl production, MPO activity and O 2 ·− production. Phagocytosis and CD11b mRNA levels were increased, while inflammatory cytokines release remained unmodified. mRNA levels of TLR4 and IκK were enhanced. Treatment of obese rats with GT increased neutrophil migration, MPO activity, H2O2, HOCl and O 2 ·− production, whereas TNF-α and IL-6 were decreased (versus obese). Similar reductions in TLR4, IκK and CD11b mRNA were observed. Catalase and hexokinase were increased by obesity, while SOD and G6PDH were decreased. Treatment with GT reduced catalase and increased the GSH/GSSG ratio.

Conclusion

In response to a cafeteria diet, we found a decreased chemotaxis, H2O2 release, MPO activity and HOCl production. We also showed a significant immunomodulatory effect of GT on the obese condition recovering some of these factors such H2O2 and HOCl production, also reducing the levels of inflammatory cytokines.

Keywords

Obesity Immune system Polyphenols Reactive oxygen species Flavonoids Catechins 

Notes

Acknowledgments

The authors are indebted to the constant assistance of Macedo, RCS, and Molina, N. This research was supported by the São Paulo Research Foundation (FAPESP, Process No. 2011/19216-8), Cruzeiro do Sul University and the National Council for Scientific and Technological Development (CNPq).

Conflict of interest

None.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • K. F. F. S. Albuquerque
    • 1
  • M. P. Marinovic
    • 1
    • 2
  • A. C. Morandi
    • 1
    • 2
  • A. P. Bolin
    • 1
  • R. Otton
    • 1
    • 2
    Email author
  1. 1.Department of Pharmacology, Biomedical Sciences InstituteUniversity of São PauloSão PauloBrazil
  2. 2.Postgraduate Programme, Health Sciences, CBSUniversidade Cruzeiro do SulSão PauloBrazil

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