Multiplatform metabolomic fingerprinting as a tool for understanding hypercholesterolemia in Wistar rats
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The aim was to investigate the impact of hypercholesterolemic diet on the metabolome of male Wistar rats by a multiplatform metabolomic fingerprinting.
Male Wistar rats were fed with two different diets [control (C) and high-cholesterol diet (HC)—containing 2 % cholesterol and 0.5 % cholic acid]. After 7 weeks of experimental feeding, the rats were euthanized for blood collection and plasma recovery. The metabolite fingerprint was then achieved by applying a multiplatform comprising LC–MS, GC–MS and CE–MS.
Multivariate statistical analysis showed a clear separation between the C and HC groups. Individual differences in metabolites were evaluated using univariate statistical analysis, and multiple metabolites were identified and confirmed in the plasma. A global profiling integrates for the first time pathways affected by high-cholesterol diet intake and allowed us to elucidate some of the associated alterations underlying the hypercholesterolemia event in Wistar rats.
HC feeding stimulated the alteration of multiple pathways in Wistar rats, warning of the risk of developing important diseases, which can be modulated by the diet. Further studies are required to investigate the possibilities to revert or ameliorate the negative effects triggered by HC intake.
KeywordsAtherosclerosis Global profiling High-cholesterol diet Metabolomics Untargeted analysis
The study was supported by the Spanish Ministry of Science and Innovation [AGL2010-15910 (subprogram ALI)] and the Spanish Ministry of Economy and Competitiveness (CTQ2014-55279-R). The following projects are also acknowledged: Program Consolider-Ingenio 2010, FUN-C-FOOD, CSD2007-00063 (Spanish Ministry of Science and Innovation), and ALIBIRD, S2009/AGR-1469 (Comunidad de Madrid).
Conflict of interest
The authors declare no conflict of interest.
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