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European Journal of Nutrition

, Volume 54, Issue 1, pp 101–107 | Cite as

Effects of a caloric restriction weight loss diet on tryptophan metabolism and inflammatory biomarkers in overweight adults

  • Barbara StrasserEmail author
  • Ken Berger
  • Dietmar Fuchs
Original Contribution

Abstract

Purpose

Recent data suggest that chronic low-grade inflammation, a characteristic of obesity, is associated with altered tryptophan (Trp) and tyrosine (Tyr) metabolism and plays a role in neuropsychiatric symptoms. The present study assessed the effect of an extreme short-term diet on Trp breakdown and inflammatory biomarkers in overweight adults.

Methods

Thirty-eight overweight participants (16 women, 22 men; average body mass index: 29 kg/m2, mean age 52.8 years) were randomized into two diet groups: a very low kcal diet group (VLCD; Ø 600 kcal/day, n = 21) and a low kcal diet group (LCD; Ø 1,200 kcal/day, n = 17). Assays included the measurement of Trp, kynurenine (Kyn), and their ratio, neopterin, phenylalanine (Phe), Tyr, as biologic markers; leptin, plasma insulin, glucose, and homeostatic model assessment-insulin resistance; and interleukin 6, tumor necrosis factor alpha, and C-reactive protein, as biochemical and inflammatory markers at baseline and after 2 weeks of treatment.

Results

Weight loss diet lowered leptin levels in both groups by 46 %, although not reaching significance. Trp and Kyn decreased significantly by 21 and 16 % for VLCD and by 15 and 17 % for the LCD group, respectively. A significant reduction in Phe was only seen after VLCD. Inflammatory biomarkers, neopterin, and Tyr were not significantly altered during the study period. Leptin was significantly correlated with Trp breakdown before and after the intervention (P < 0.02).

Conclusions

Since disturbed metabolism of Trp affects biosynthesis of serotonin and might be associated with increased susceptibility for mood disturbances and carbohydrate craving, strategies to supplement Trp while dieting could be highly useful in treating uncontrolled weight gain or in preventing neuropsychiatric symptoms.

Keywords

Diet Leptin Tryptophan Inflammation Mood 

Notes

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

References

  1. 1.
    Engström G, Hedblad B, Stavenow L, Jonsson S, Lind P, Janzon L, Lindgärde F (2004) Incidence of obesity-associated cardiovascular disease is related to inflammation-sensitive plasma proteins: a population-based cohort study. Arterioscler Thromb Vasc Biol 24:1498–1502CrossRefGoogle Scholar
  2. 2.
    Dyck DJ (2009) Adipokines as regulators of muscle metabolism and insulin sensitivity. Appl Physiol Nutr Metab 34:396–402CrossRefGoogle Scholar
  3. 3.
    Wang P, Mariman E, Renes J, Keijer J (2008) The secretory function of adipocytes in the physiology of white adipose tissue. J Cell Physiol 216:3–13CrossRefGoogle Scholar
  4. 4.
    Brandacher G, Hoeller E, Fuchs D, Weiss HG (2007) Chronic immune activation underlies morbid obesity: is IDO a key player? Curr Drug Metab 8:289–295CrossRefGoogle Scholar
  5. 5.
    Nduhirabandi F, du Toit EF, Lochner A (2012) Melatonin and the metabolic syndrome: a tool for effective therapy in obesity-associated abnormalities? Acta Physiol 205:209–223CrossRefGoogle Scholar
  6. 6.
    Mangge H, Summers KL, Meinitzer A, Zelzer S, Almer G, Prassl R, Schnedl WJ, Reininghaus E, Paulmichl K, Weghuber D, Fuchs D (2013) Obesity-related dysregulation of the Tryptophan-Kynurenine metabolism: Role of age and parameters of the metabolic syndrome. Obesity. doi: 10.1002/oby.20491
  7. 7.
    Capuron L, Schroecksnadel S, Féart C, Aubert A, Higueret D, Barberger-Gateau P, Layé S, Fuchs D (2011) Chronic low-grade inflammation in elderly persons is associated with altered tryptophan and tyrosine metabolism: role in neuropsychiatric symptoms. Biol Psychiatry 70:175–182CrossRefGoogle Scholar
  8. 8.
    Capuron L, Ravaud A, Neveu PJ, Miller AH, Maes M, Dantzer R (2007) Association between decreased serum tryptophan concentrations and depressive symptoms in cancer patients undergoing cytokine therapy. Mol Psychiatry 7:468–473CrossRefGoogle Scholar
  9. 9.
    Fontana L, Klein S (2007) Aging, adiposity, and calorie restriction. JAMA 297:986–994CrossRefGoogle Scholar
  10. 10.
    Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28:412–419CrossRefGoogle Scholar
  11. 11.
    Neurauter G, Scholl-Bürgi S, Haara A, Geisler S, Mayersbach P, Schennach H, Fuchs D (2013) Simultaneous measurement of phenylalanine and tyrosine by high performance liquid chromatography (HPLC) with fluorescence detection. Clin Biochem 46:1848–1851CrossRefGoogle Scholar
  12. 12.
    Blüher M, Rudich A, Klöting N, Golan R, Henkin Y, Rubin E, Schwarzfuchs D, Gepner Y, Stampfer MJ, Fiedler M, Thiery J, Stumvoll M, Shai I (2012) Two patterns of adipokine and other biomarker dynamics in a long-term weight loss intervention. Diabetes Care 35:342–349CrossRefGoogle Scholar
  13. 13.
    Itoh K, Imai K, Masuda T, Abe S, Tanaka M, Koga R, Itoh H, Matsuyama T, Nakamura M (2002) Relationship between changes in serum leptin levels and blood pressure after weight loss. Hypertens Res 25:881–886CrossRefGoogle Scholar
  14. 14.
    Oberhauser F, Schulte DM, Faust M, Güdelhöfer H, Hahn M, Müller N, Neumann K, Krone W, Laudes M (2012) Weight loss due to a very low calorie diet nondiabetic obese human subjects. Horm Metab Res 44:465–470CrossRefGoogle Scholar
  15. 15.
    Ruge T, Lockton JA, Renstrom F, Lystig T, Sukonina V, Svensson MK, Eriksson JW (2009) Acute hyperinsulinemia raises plasma interleukin-6 in both nondiabetic and type 2 diabetes mellitus subjects, and this effect is inversely associated with body mass index. Metabolism 58:860–866CrossRefGoogle Scholar
  16. 16.
    Häfner S, Zierer A, Emeny RT, Thorand B, Herder C, Koenig W, Rupprecht R, Ladwig KH, KORA Study Investigators (2011) Social isolation and depressed mood are associated with elevated serum leptin levels in men but not in women. Psychoneuroendocrinology 36:200–209CrossRefGoogle Scholar
  17. 17.
    Widner B, Laich A, Sperner-Unterweger B, Ledochowski M, Fuchs D (2002) Neopterin production tryptophan degradation and mental depression: what is the link? Brain Behav Immun 16:590–595CrossRefGoogle Scholar
  18. 18.
    Reibnegger G, Vrecko K, Mlekusch W, Lamprecht M (1997) Influence of three different diet modes combined with exercise loads on urinary and plasma neopterin concentrations: a longitudinal study. Pteridines 8:211–215Google Scholar
  19. 19.
    Anderson IM, Parry-Billings M, Newsholme EA, Fairburn CH, Cowen PJ (1990) Dieting reduces plasma tryptophan and alters brain 5-HT function in women. Psychol Med 20:785–791CrossRefGoogle Scholar
  20. 20.
    Raison CL, Dantzer R, Kelley KW, Lawson MA, Woolwine BJ, Vogt G, Spivey JR, Saito K, Miller AH (2010) CSF concentrations of brain tryptophan and kynurenines during immune stimulation with IFN-alpha: relationship to CNS immune responses and depression. Mol Psychiatry 15:393–403CrossRefGoogle Scholar
  21. 21.
    Dantzer R, O’Connor JC, Lawson MA, Kelley KW (2011) Inflammation-associated depression: from serotonin to kynurenine. Psychoneuroendocrinology 36:426–436CrossRefGoogle Scholar
  22. 22.
    Young SN (2013) The effect of raising and lowering tryptophan levels on human mood and social behaviour. Philos Trans R Soc Lond B Biol Sci 368:20110375CrossRefGoogle Scholar
  23. 23.
    Gatti E, Porrini M, Noe D, Crovetti R, Testolin G (1994) Plasma amino acids changes in obese patients on very low-calorie diets. Int J Vitam Nutr Res 64:81–85Google Scholar
  24. 24.
    Wurtman RJ, Wurtman JJ (1995) Brain serotonin, carbohydrate-craving, obesity and depression. Obes Res 3:477S–480SCrossRefGoogle Scholar
  25. 25.
    Amigo I, Fernández C (2007) Effects of diets and their role in weight control. Psychol Health Med 12:321–327CrossRefGoogle Scholar
  26. 26.
    Kajioka T, Tsuzuku S, Shimokata H, Sato Y (2002) Effects of intentional weight cycling on non-obese young women. Metabolism 51:149–154CrossRefGoogle Scholar
  27. 27.
    Banasik JL, Walker MK, Randall JM, Netjes RB, Foutz MS (2013) Low-calorie diet induced weight loss may alter regulatory hormones and contribute to rebound visceral adiposity in obese persons with a family history of type-2 diabetes. J Am Assoc Nurse Pract 25:440–448Google Scholar
  28. 28.
    Mason C, Foster-Schubert KE, Imayama I, Xiao L, Kong A, Campbell KL, Duggan CR, Wang CY, Alfano CM, Ulrich CM, Blackburn GL, McTiernan A (2013) History of weight cycling does not impede future weight loss or metabolic improvements in postmenopausal women. Metabolism 62:127–136CrossRefGoogle Scholar
  29. 29.
    Parker G, Brotchie H (2011) Mood effects of the amino acids tryptophan and tyrosine: ‘Food for Thought’ III. Acta Psychiatr Scand 124:417–426CrossRefGoogle Scholar
  30. 30.
    Chen Y, Guillemin GJ (2009) Kynurenine pathway metabolites in humans: disease and healthy states. Int J Tryptophan Res 2:1–19Google Scholar
  31. 31.
    Dekker MJ, Lee S, Hudson R, Kilpatrick K, Graham TE, Ross R, Robinson LE (2007) An exercise intervention without weight loss decreases circulating interleukin-6 in lean and obese men with and without type 2 diabetes mellitus. Metabolism 56:332–338CrossRefGoogle Scholar
  32. 32.
    Balducci S, Zanuso S, Nicolucci A, Fernando F, Cavallo S, Cardelli P, Fallucca S, Alessi E, Letizia C, Jimenez A, Fallucca F, Pugliese G (2010) Anti-inflammatory effect of exercise training in subjects with type 2 diabetes and the metabolic syndrome is dependent on exercise modalities and independent of weight loss. Nutr Metab Cardiovasc Dis 20:608–617CrossRefGoogle Scholar
  33. 33.
    Strasser B, Arvandi M, Siebert U (2012) Resistance training, visceral obesity and inflammatory response: a review of the evidence. Obes Rev 13:578–591CrossRefGoogle Scholar
  34. 34.
    Dey S, Singh RH, Dey PK (1992) Exercise training: significance of regional alterations in serotonin metabolism of rat brain in relation to antidepressant effect of exercise. Physiol Behav 52:1095–1099CrossRefGoogle Scholar
  35. 35.
    Meeusen R, Thorré K, Chaouloff F, Sarre S, De Meirleir K, Ebinger G, Michotte Y (1996) Effects of tryptophan and/or acute running on extracellular 5-HT and 5-HIAA levels in the hippocampus of food-deprived rats. Brain Res 740:245–252CrossRefGoogle Scholar
  36. 36.
    Dunn AL, Trivedi MH, Kampert JB, Clark CG, Chambliss HO (2005) Exercise treatment for depression: efficacy and dose response. Am J Prev Med 28:1–8CrossRefGoogle Scholar
  37. 37.
    Babyak M, Blumenthal JA, Herman S, Khatri P, Doraiswamy M, Moore K, Craighead WE, Baldewicz TT, Krishnan KR (2000) Exercise treatment for major depression: maintenance of therapeutic benefit at 10 months. Psychosom Med 62:633–638CrossRefGoogle Scholar
  38. 38.
    Blake CE, Hébert JR, Lee DC, Adams SA, Steck SE, Sui X, Kuk JL, Baruth M, Blair SN (2013) Adults with greater weight satisfaction report more positive health behaviors and have better health status regardless of BMI. J Obes 2013:291371CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institute for Nutritional Sciences and PhysiologyUniversity for Health Sciences, Medical Informatics and TechnologyHall in TirolAustria
  2. 2.Division of Biological Chemistry, BiocenterMedical University InnsbruckInnsbruckAustria

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