Effect of white tea (Camellia sinensis (L.)) extract in the glycolytic profile of Sertoli cell
- 464 Downloads
Many health benefits have been attributed to tea (Camellia sinensis (L.)), and tea infusions are used as dietary agent and included in food supplements. Herein, we report the effect of a white tea (WTEA) extract in Sertoli cell (SC) metabolism. The SC is responsible for the nutritional support of the developing germ cells.
An aqueous WTEA extract was prepared and analyzed by 1H-NMR. Rat SCs were cultured with or without the WTEA extract. mRNA and protein levels of glucose transporters (GLUT1 and GLUT3), phosphofructokinase, lactate dehydrogenase (LDH) and monocarboxylate transporter 4 were determined by qPCR and western blot. LDH activity was assessed and metabolite production/consumption determined by 1H-NMR.
WTEA-exposed SCs presented decreased protein and mRNA levels of GLUT1 and decreased glucose uptake. However, intracellular LDH activity was increased and SC lactate production was stimulated by the presence of the WTEA extract. Interestingly, alanine production was also found to be stimulated in WTEA extract-exposed SCs.
WTEA extract altered the glycolytic profile of cultured SCs, stimulating lactate production. Since lactate is used as metabolic substrate and has an anti-apoptotic effect in the developing germ cells, the supplementation with WTEA extract may be advantageous to improve male reproductive health.
KeywordsWhite tea Camellia sinensis (L.) Sertoli Metabolism Lactate
This work was supported by the “Fundação para a Ciência e a Tecnologia”—FCT (PTDC/QUI-BIQ/121446/2010 and PEst-C/SAU/UI0709/2011) co-funded by Fundo Europeu de Desenvolvimento Regional—FEDER via Programa Operacional Factores de Competitividade—COMPETE/QREN. MG Alves (SFRH/BPD/80451/2011) was funded by FCT. PF Oliveira was funded by FCT through FSE and POPH funds (Programa Ciência 2008).
Conflict of interest
The authors declare that they have no conflict of interest.
- 4.Hininger-Favier I, Benaraba R, Coves S, Anderson RA, Roussel AM (2009) Green tea extract decreases oxidative stress and improves insulin sensitivity in an animal model of insulin resistance, the fructose-fed rat. J Am Coll Nutr 28(4):355–361. doi: 10.1080/07315724.2009.10718097 CrossRefGoogle Scholar
- 13.Alves MG, Socorro S, Silva J, Barros A, Sousa M, Cavaco JE, Oliveira PF (2012) In vitro cultured human Sertoli cells secrete high amounts of acetate that is stimulated by 17beta-estradiol and suppressed by insulin deprivation. Biochim Biophys Acta 1823(8):1389–1394. doi: 10.1016/j.bbamcr.2012.06.002 CrossRefGoogle Scholar
- 14.Oliveira PF, Alves MG, Rato L, Laurentino S, Silva J, Sa R, Barros A, Sousa M, Carvalho RA, Cavaco JE, Socorro S (2012) Effect of insulin deprivation on metabolism and metabolism-associated gene transcript levels of in vitro cultured human Sertoli cells. Biochim Biophys Acta 1820(2):84–89. doi: 10.1016/j.bbagen.2011.11.006 CrossRefGoogle Scholar
- 24.Galardo MN, Riera MF, Pellizzari EH, Chemes HE, Venara MC, Cigorraga SB, Meroni SB (2008) Regulation of expression of Sertoli cell glucose transporters 1 and 3 by FSH, IL1 beta, and bFGF at two different time-points in pubertal development. Cell Tissue Res 334(2):295–304. doi: 10.1007/s00441-008-0656-y CrossRefGoogle Scholar
- 25.Dias TR, Tomás G, Teixeira N, Alves MG, Oliveira PF, Silva BM (2013) White tea (Camellia Sinensis (L.)): antioxidant properties and beneficial health effects. Int J Food Sci Nutr Diet 2:1–15Google Scholar
- 27.Williams SN, Shih H, Guenette DK, Brackney W, Denison MS, Pickwell GV, Quattrochi LC (2000) Comparative studies on the effects of green tea extracts and individual tea catechins on human CYP1A gene expression. Chem Biol Interact 128(3):211–229. doi: 10.1016/S0009-2797(00)00204-0 CrossRefGoogle Scholar
- 38.Martins AD, Alves MG, Simoes VL, Dias TR, Rato L, Moreira PI, Socorro S, Cavaco JE, Oliveira PF (2013) Control of Sertoli cell metabolism by sex steroid hormones is mediated through modulation in glycolysis-related transporters and enzymes. Cell Tissue Res 354(3):861–868. doi: 10.1007/s00441-013-1722-7 CrossRefGoogle Scholar
- 46.Sukhthankar M, Alberti S, Baek SJ (2010) (–)-Epigallocatechin-3-gallate (EGCG) post-transcriptionally and post-translationally suppresses the cell proliferative protein TROP2 in human colorectal cancer cells. Anticancer Res 30(7):2497–2503Google Scholar