European Journal of Nutrition

, Volume 53, Issue 3, pp 973–980 | Cite as

Red grape berry-cultured cells reduce blood pressure in rats with metabolic-like syndrome

  • A. Leibowitz
  • Z. Faltin
  • A. Perl
  • Y. Eshdat
  • Y. Hagay
  • E. Peleg
  • E. Grossman
Original Contribution



Cumulative evidence suggests that moderate red wine consumption protects the cardiovascular system. The effect of cultured cells derived from red grape berry (RGC) on blood pressure (BP) has not been investigated. We therefore studied the antihypertensive effects of oral consumption of RGC in experimental rat model of metabolic-like syndrome and assessed its effect on human umbilical vein endothelial cells (HUVECs).


Forty male Sprague–Dawley rats were fed for 5 weeks with either a high fructose diet (HFD) (n = 10) or HFD supplemented, during the last 2 weeks, with different doses (200, 400 and 800 mg/kg/day) of RGC suspended in their food (n = 30). BP, plasma triglycerides, insulin and adiponectin levels were measured at the beginning and after 3 and 5 weeks of diet. RGC effect on vasodilatation was evaluated by its ability to affect endothelin-1 (ET-1) production and endothelial nitric oxide synthase (eNOS) expression in HUVECs.


BP, plasma triglycerides, insulin and adiponectin increased significantly in rats fed with a HFD. The increase in BP, plasma triglycerides and insulin was attenuated by RGC supplementation. Incubation of HUVECs with RGC demonstrated a concentration-dependent inhibition of ET-1 secretion and increase in the level of eNOS, signaling a positive effect of RGC on vasodilatation.


In rats with metabolic-like syndrome, RGC decreased BP and improved metabolic parameters. These beneficial effects may be mediated by the cell constituents, highly rich with polyphenols and resveratrol, reside in their natural state.


Rats Metabolic syndrome Blood pressure Red grape-cultured cell Endothelin-1 Endothelial NO synthase 


Conflict of interest

Dr. Avshhalom Leibowitz, Dr. Edna Peleg and Dr. Ehud Grossman received a research grant from Fruitura (formly Hi-Nutra) Bioscience Ltd. Rehovot, Israel.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • A. Leibowitz
    • 1
    • 2
  • Z. Faltin
    • 3
  • A. Perl
    • 3
  • Y. Eshdat
    • 3
  • Y. Hagay
    • 4
  • E. Peleg
    • 1
    • 2
  • E. Grossman
    • 1
    • 2
  1. 1.Internal Medicine D and the Hypertension UnitThe Chaim Sheba Medical CenterTel-HashomerIsrael
  2. 2.Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael
  3. 3.Institute of Plant Science, Agricultural Research OrganizationThe Volcani CenterBet DaganIsrael
  4. 4.Fruitura BioscienceRehovotIsrael

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