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European Journal of Nutrition

, Volume 53, Issue 3, pp 813–821 | Cite as

Long-term dietary l-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine

  • Ksenija Velickovic
  • Milica Markelic
  • Igor Golic
  • Vesna Otasevic
  • Ana Stancic
  • Aleksandra Jankovic
  • Milica Vucetic
  • Biljana Buzadzic
  • Bato Korac
  • Aleksandra KoracEmail author
Original Contribution

Abstract

Background and aims

Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are important intestinal neurotransmitters that coexist in the gut enteric nervous system and play an important role in intestinal physiology (e.g., absorption, motility, fluid secretion and smooth muscle relaxation). It is also known that cold exposure alters several aspects of gastrointestinal physiology and induces hyperphagia to meet increased metabolic demands, but there are no data regarding NO and VIP involvement in intestinal response during acclimation to cold. The objective of this study was to determine the influence of long-term l-arginine supplementation on the expression of the three isoforms of nitric oxide synthase (NOS) and VIP in small intestine of rats acclimated to room temperature or cold.

Methods

Animals (six per group) acclimated to room temperature (22 ± 1 °C) and cold (4 ± 1 °C), respectively, were treated with 2.25 % l-arginine, a substrate for NOSs, or with 0.01 % N ω-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45 days. The topographical distribution of VIP and NOSs expression in small intestine was studied by immunohistochemistry, and ImageJ software was used for semiquantitative densitometric analysis of their immunoexpression.

Results

Long-term dietary l-arginine supplementation increases VIP and NOSs immunoexpression at room temperature while at cold increases the endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat small intestine.

Conclusion

Our results demonstrate that long-term dietary l-arginine supplementation modulates NOSs and VIP immunoexpression in rat small intestine with respect to ambient temperature, pointing out the eNOS as a predominant NOS isoform with an immunoexpression pattern similar to VIP.

Keywords

Arginine Small intestine Nitric oxide synthase Vasoactive intestinal peptide 

Abbreviations

NO

Nitric oxide

NOS

Nitric oxide synthase

eNOS

Endothelial NOS

iNOS

Inducible NOS

nNOS

Neuronal NOS

VIP

Vasoactive intestinal peptide

l-NAME

N ω-nitro-l-arginine methyl ester

ICC

Interstitial cells of Cajal

Notes

Acknowledgments

This research was supported by the Grant from Serbian Ministry of Education, Science and Technological Development, #173055.

Conflict of interest

The authors declare that they have no conflict of interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ksenija Velickovic
    • 1
  • Milica Markelic
    • 1
  • Igor Golic
    • 1
  • Vesna Otasevic
    • 2
  • Ana Stancic
    • 2
  • Aleksandra Jankovic
    • 2
  • Milica Vucetic
    • 2
  • Biljana Buzadzic
    • 2
  • Bato Korac
    • 2
  • Aleksandra Korac
    • 1
    Email author
  1. 1.Faculty of BiologyUniversity of BelgradeBelgradeSerbia
  2. 2.Institute for Biological Research “Sinisa Stankovic”University of BelgradeBelgradeSerbia

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