European Journal of Nutrition

, Volume 53, Issue 2, pp 549–555

Validity of food frequency questionnaire-based estimates of long-term long-chain n-3 polyunsaturated fatty acid intake

  • Alice Wallin
  • Daniela Di Giuseppe
  • Ann Burgaz
  • Niclas Håkansson
  • Tommy Cederholm
  • Karl Michaëlsson
  • Alicja Wolk
Original Contribution

DOI: 10.1007/s00394-013-0563-1

Cite this article as:
Wallin, A., Di Giuseppe, D., Burgaz, A. et al. Eur J Nutr (2014) 53: 549. doi:10.1007/s00394-013-0563-1

Abstract

Purpose

To evaluate how long-term dietary intake of long-chain n-3 polyunsaturated fatty acids (LCn-3 PUFAs), estimated by repeated food frequency questionnaires (FFQs) over 15 years, is correlated with LCn-3 PUFAs in adipose tissue (AT).

Methods

Subcutaneous adipose tissue was obtained in 2003–2004 (AT-03) from 239 randomly selected women, aged 55–75 years, after completion of a 96-item FFQ (FFQ-03). All participants had previously returned an identical FFQ in 1997 (FFQ-97) and a 67-item version in 1987–1990 (FFQ-87). Pearson product-moment correlations were used to evaluate associations between intake of total and individual LCn-3 PUFAs as estimated by the three FFQ assessments and AT-03 content (% of total fatty acids).

Results

FFQ-estimated mean relative intake of LCn-3 PUFAs (% of total fat intake) increased between all three assessments (FFQ-87, 0.55 ± 0.34; FFQ-97, 0.74 ± 0.64; FFQ-03, 0.88 ± 0.56). Validity, in terms of Pearson correlations between FFQ-03 estimates and AT-03 content, was 0.41 (95 % CI 0.30–0.51) for total LCn-3 PUFA and ranged from 0.29 to 0.48 for individual fatty acids; lower correlation was observed among participants with higher percentage body fat. With regard to long-term intake estimates, past dietary intake was also correlated with AT-03 content, with correlation coefficients in the range of 0.21–0.33 and 0.21–0.34 for FFQ-97 and FFQ-87, respectively. The correlations were improved by using average estimates from two or more FFQ assessments. Exclusion of fish oil supplement users (14 %) did not alter the correlations.

Conclusion

These data indicate reasonable validity of FFQ-based estimates of long-term (up to 15 years) LCn-3 PUFA intake, justifying their use in studies of diet-disease associations.

Keywords

Adipose tissue Dietary assessment Food frequency questionnaire Long-chain n-3 fatty acids Validation 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Alice Wallin
    • 1
  • Daniela Di Giuseppe
    • 1
  • Ann Burgaz
    • 1
  • Niclas Håkansson
    • 1
  • Tommy Cederholm
    • 2
  • Karl Michaëlsson
    • 3
  • Alicja Wolk
    • 1
  1. 1.Division of Nutritional Epidemiology, Institute of Environmental MedicineKarolinska InstitutetStockholmSweden
  2. 2.Department of Public Health and Caring Sciences, Clinical Nutrition and MetabolismUppsala UniversityUppsalaSweden
  3. 3.Department of Surgical Sciences, Section of OrthopedicsUppsala UniversityUppsalaSweden

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