European Journal of Nutrition

, Volume 53, Issue 1, pp 211–219

Dietary fat quality in regular fat diets has minor effects on biomarkers of inflammation in obese Zucker rats

  • Daniela Graf
  • Stephan W. Barth
  • Achim Bub
  • Judith Narr
  • Corinna E. Rüfer
  • Bernhard Watzl
  • Stephanie Seifert
Original Contribution

Abstract

Purpose

Adipose tissue-associated chronic inflammation is involved in the pathogenesis of obesity-related diseases. Dietary fatty acids are known to influence inflammatory processes. The aim of this study was to investigate, whether diets with regular fat contents but variable fat qualities affect adipose tissue-associated inflammation through the fatty acid composition of mesenteric adipose tissue (MAT).

Methods

Obese Zucker rats were fed diets containing 7 % wt:wt rapeseed oil, corn oil, or lard for 10 weeks. Fatty acid composition and endocrine function regarding adipokines and cytokines of MAT, number of total CD3+ T cells, and cytokine secretion of mesenteric lymph node (MLN)-derived lymphocytes were determined. Local effects in MAT and MLN were compared to systemic effects assessed in serum and peripheral blood mononuclear cells.

Results

Fatty acid composition of MAT reflected dietary fatty acid intake, without affecting endocrine function. Feeding the lard diet for 10 weeks increased the serum adiponectin and TNF-α secretion of blood lymphocytes, whereas CD3+ T cells in blood were decreased. No effects were seen for the secretion of adipokines and cytokines from MAT, the amount of T cells in MLN, and cytokine secretion of MLN lymphocytes.

Conclusions

In conclusion, feeding obese rats a diet with regular fat content but variable fat sources for 10 weeks, changed the fatty acid composition of MAT but not its secretory properties or MLN functions. Although the local immune system was not influenced, lard-feeding induced minor changes in systemic immune function.

Keywords

Inflammation Fat quality Obesity Immune function 

Abbreviations

AA

Arachidonic acid

ALA

α-Linolenic acid

AT

Adipose tissue

RSO

Rapeseed oil

COO

Corn oil

CRP

C-reactive protein

FITC

Fluorescein isothiocyanate

HFD

High-fat diet

LA

Linoleic acid

MAT

Mesenteric adipose tissue

MCP-1

Monocyte chemotactic protein-1

MLN

Mesenteric lymph node

MUFA

Monounsaturated fatty acids

PAI-1

Plasminogen activator inhibitor-1

PBMC

Peripheral blood mononuclear cells

PE

Phycoerythrin

PUFA

Polyunsaturated fatty acids

SFA

Saturated fatty acids

TMSH

Trimethylsulfonium hydroxide

Supplementary material

394_2013_518_MOESM1_ESM.docx (27 kb)
Supplementary material 1 (DOCX 27 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Daniela Graf
    • 1
  • Stephan W. Barth
    • 1
  • Achim Bub
    • 1
  • Judith Narr
    • 1
  • Corinna E. Rüfer
    • 2
  • Bernhard Watzl
    • 1
  • Stephanie Seifert
    • 1
  1. 1.Department of Physiology and Biochemistry of Nutrition, Max Rubner-InstitutFederal Research Institute of Nutrition and FoodKarlsruheGermany
  2. 2.Department of Safety and Quality of Fruits and Vegetables, Max Rubner-InstitutFederal Research Institute of Nutrition and FoodKarlsruheGermany

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