Absorption and isomerization of caffeoylquinic acids from different foods using ileostomist volunteers
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Polyphenols are thought to play important roles in human nutrition and health but these health effects are dependent on their bioavailability. This study is one of a series with the aim of determining possible effects of food matrices on caffeoylquinic acid (CQA) bioavailability using ileostomy volunteers.
After a CQA-free diet, ileostomists consumed coffee (746 μmol total CQA), and CQAs in excreted ileal fluid were subsequently identified and quantified with HPLC–diode array detection and HPLC-ESI-MS/MS. In our previous studies, other food sources such as cloudy apple juice (CAJ) (358 μmol CQA) and apple smoothie (AS) (335 μmol CQA) were investigated with the same model.
Interesterification of CQA from both apple matrices was observed during gastrointestinal passage, whereas CQA consumed in coffee was not influenced by interesterification reactions. In total, 74.3, 22.4, and 23.8 % of the CQA from CAJ, AS, and coffee, respectively, were absorbed or degraded.
Our results show that variations in food matrices and variations in phenolic composition have a major influence on intestinal bioavailability and interesterification of the investigated subclass of polyphenols, the CQAs.
KeywordsCaffeoylquinic acid Apple juice Apple smoothie Coffee Bioavailability Ileostomy
- pKa (37 °C)
Ionization constant in water
- diff(log PN−I)
Difference between log P N and log P I
- log PI
Logarithm of the partition coefficient of a given compound in its fully ionized form
- log PN
Logarithm of the partition coefficient of a given compound in its neutral form
- log D6.8
Logarithm of the distribution coefficient at pH 6.8
Multidrug resistance protein 2
Cloudy apple juice
The authors thank all volunteers in the study for their participation; Lionel Philippe (NRC) for supervising the study as clinical project manager and Ines Holub for her widespread help in performing the intervention studies in Wuerzburg. The study was funded by the Nestlé Research Centre, NRC (Lausanne, Switzerland).
Conflict of interest
The authors declare no commercial or financial conflict of interest.
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